A Gold Nanoparticle and Aflatoxin B1-BSA Conjugates Based Lateral Flow Assay Method for the Analysis of Aflatoxin B1

Abstract

A rapid and simple immuno-chromatographic assay was developed to detect aflatoxin B1 (AFB1). The assay was based on a modified competitive binding format using colloidal gold and polyclonal antibody (Pab) conjugates. The anti-AFB1 Pab was immobilized to a defined detection zone on a porous nitrocellulose membrane and colloidal gold particles were conjugated to AFB1-BSA which served as a detection reagent. The AFB1-containing sample was added to the membrane and allowed to move with AFB1-BSA-coated particles dried on the conjugation pad. The mixture was then passed along the porous membrane by capillary action past the Pab in the detection zone, which captured AFB1 or AFB1-BSA. AFB1 in the sample inhibits binding of AFB1-BSA conjugated gold particles to the Pab and prevents formation of a red color dot. In the absence of AFB1, AFB1-BSA conjugated gold particles bound to the Pab, give a red color within this detection zone. With this method, 10 μg/mL of AFB1 was detected in less than 10 min. The developed AFB1 assay also showed no cross reaction to Ochratoxin A (OTA).

Performance Improvement of the One-Dot Lateral Flow Immunoassay for Aflatoxin B1 by Using a Smartphone-Based Reading System

“…[17]. The colloidal gold-AFB1-BSA and antibody concentrations were modified to achieve better sensitivity and detection limits.…”

“…The sample pad, nitrocellulose membrane, and conjugation pad were prepared, as previously described [17]. The sample pad was treated with 50 mM borate buffer, pH 7.4, containing 1% BSA and 0.05% Tween-20, and then dried overnight at 37 °C.…”

Sensitive detection of aflatoxin B1 molecules on gold SPR chip surface using functionalized gold nanoparticles

“…This direct method thus is suitable for determination of AFB1 in important alimentary target compounds by functionalized AuNPs. An additional advantage is that no additional reagents are needed for the analysis as compared to previous studies (Daly et al 2000;Dunne et al 2005;Moon et al 2012). …”

A Review on Lateral Flow Test Strip for Food Safety

“…Either way the analytes in the sample compete with the standard analytes. Moon et al (2012) utilized the competitive assay to detect aflatoxin B1 (AFB1). In this study, colloidal gold particles were conjugated to the AFB1-bovine serum albumin (BSA) and dried on the conjugation pad to serve as labeled probes.…”