A glycosylphosphatidylinositol anchor signal sequence enhances the immunogenicity of a DNA vaccine encoding Plasmodium falciparum sexual-stage antigen, Pfs230

Fanning, Sarah; Czesny, Beata; Carucci, Daniel J.; Gemert, Geert Jan van; Eling, W.M.C.

doi:10.1016/s0264-410x(03)00265-2

Cited by 25 publications

(9 citation statements)

References 27 publications

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“…24,2011 P. FALCIPARUM AND P. VIVAX GAMETOCYTES 393 (517). DNA vaccination with the immunogenic region C of Pfs230 with an additional GPI anchor also induced high IgG titers in mice (145). Because of its size and the difficulty in obtaining correctly folded protein, an alternative approach producing parts of the protein in tobacco plants is currently used (501).…”

Section: Current Status Of Transmission-blocking Vaccinesmentioning

confidence: 99%

Epidemiology and Infectivity of Plasmodium falciparum and Plasmodium vivax Gametocytes in Relation to Malaria Control and Elimination

2011

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SUMMARY Malaria remains a major cause of morbidity and mortality in the tropics, with Plasmodium falciparum responsible for the majority of the disease burden and P. vivax being the geographically most widely distributed cause of malaria. Gametocytes are the sexual-stage parasites that infect Anopheles mosquitoes and mediate the onward transmission of the disease. Gametocytes are poorly studied despite this crucial role, but with a recent resurgence of interest in malaria elimination, the study of gametocytes is in vogue. This review highlights the current state of knowledge with regard to the development and longevity of P. falciparum and P. vivax gametocytes in the human host and the factors influencing their distribution within endemic populations. The evidence for immune responses, antimalarial drugs, and drug resistance influencing infectiousness to mosquitoes is reviewed. We discuss how the application of molecular techniques has led to the identification of submicroscopic gametocyte carriage and to a reassessment of the human infectious reservoir. These components are drawn together to show how control measures that aim to reduce malaria transmission, such as mass drug administration and a transmission-blocking vaccine, might better be deployed.

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Section: Current Status Of Transmission-blocking Vaccinesmentioning

confidence: 99%

Epidemiology and Infectivity of Plasmodium falciparum and Plasmodium vivax Gametocytes in Relation to Malaria Control and Elimination

2011

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“…It is likely that it is a discontinuous epitope containing amino acids widely dispersed in the linear sequence. Therefore, a mammalian expression system (Vical plasmid VR1020) was selected to direct production of full‐length Pfs230 region C to the secretory pathway in mammalian cells (49). One construct encoded only region C (V‐230.C), while a second included the Pfs25 GPI attachment signal sequence (V‐230.C‐GPI).…”

Section: Eukaryotic Production Of Pfs230 Recombinant Proteinsmentioning

confidence: 99%

Pfs230: from malaria transmission‐blocking vaccine candidate toward function

Williamson

2003

Parasite Immunology

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“…It encodes a transmission-blocking target antigen s230 precursor (Pfs230) in P. falciparum and one single copy is present in the other five Plasmodium species. Pfs230 is expressed on the plasma membrane of parasite gametocytes in the human host and, after the parasites are taken up in a blood meal by a mosquito vector, it remains on the surface of the emerged gamete [33]. Transmission activity was found to be blocked when anti-Pfs230 antibodies were used, suggesting Pfs230 can be a potential vaccine target.…”

Section: Resultsmentioning

confidence: 99%

Core genome components and lineage specific expansions in malaria parasites Plasmodium

Cai

Wang

2010

BMC Genomics

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BackgroundThe increasing resistance of Plasmodium, the malaria parasites, to multiple commonly used drugs has underscored the urgent need to develop effective antimalarial drugs and vaccines. The new direction of genomics-driven target discovery has become possible with the completion of parasite genome sequencing, which can lead us to a better understanding of how the parasites develop the genetic variability that is associated with their response to environmental challenges and other adaptive phenotypes.ResultsWe present the results of a comprehensive analysis of the genomes of six Plasmodium species, including two species that infect humans, one that infects monkeys, and three that infect rodents. The core genome shared by all six species is composed of 3,351 genes, which make up about 22%-65% of the genome repertoire. These components play important roles in fundamental functions as well as in parasite-specific activities. We further investigated the distribution and features of genes that have been expanded in specific Plasmodium lineage(s). Abundant duplicate genes are present in the six species, with 5%-9% of the whole genomes composed lineage specific radiations. The majority of these gene families are hypothetical proteins with unknown functions; a few may have predicted roles such as antigenic variation.ConclusionsThe core genome components in the malaria parasites have functions ranging from fundamental biological processes to roles in the complex networks that sustain the parasite-specific lifestyles appropriate to different hosts. They represent the minimum requirement to maintain a successful life cycle that spans vertebrate hosts and mosquito vectors. Lineage specific expansions (LSEs) have given rise to abundant gene families in Plasmodium. Although the functions of most families remain unknown, these LSEs could reveal components in parasite networks that, by their enhanced genetic variability, can contribute to pathogenesis, virulence, responses to environmental challenges, or interesting phenotypes.

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A glycosylphosphatidylinositol anchor signal sequence enhances the immunogenicity of a DNA vaccine encoding Plasmodium falciparum sexual-stage antigen, Pfs230

Cited by 25 publications

References 27 publications

Epidemiology and Infectivity of Plasmodium falciparum and Plasmodium vivax Gametocytes in Relation to Malaria Control and Elimination

Epidemiology and Infectivity of Plasmodium falciparum and Plasmodium vivax Gametocytes in Relation to Malaria Control and Elimination

Pfs230: from malaria transmission‐blocking vaccine candidate toward function

Core genome components and lineage specific expansions in malaria parasites Plasmodium

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