A glycopeptide regulating stem cell fate in Arabidopsis thaliana

Ohyama, Kentaro; Shinohara, Hidefumi; Ogawa-Ohnishi, Mari; Matsubayashi, Yoshikatsu

doi:10.1038/nchembio.182

Cited by 377 publications

(436 citation statements)

References 15 publications

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“…This finding further supports the physiological importance of Hyp-arabinosylation in peptide signalling. Initial studies in Arabidopsis reported that arabinosylation of CLV3 glycopeptide considerably enhances its binding to CLV1 receptor kinase 26 . A recent conformational analysis of [Ara 3 ]CLV3 showed that the arabinose chain of [Ara 3 ]CLV3 extends towards the C-terminal end of the peptide and causes distinct distortion in the C-terminal half of the peptide in a highly directional manner 27 .…”

Section: Discussionmentioning

confidence: 99%

“…The resulting T3 plants (10 days after germination, DAG) were subjected to whole-plant submerged culture in 200 ml medium 24 . Secreted peptides accumulating after 24 days of culture were extracted by o-chlorophenol and fractionated in a gel-filtration column 24,26 .…”

Section: Methodsmentioning

confidence: 99%

“…Modification of Hyp residues by arabinose is a plant-specific post-translational modification of peptides synthesized through secretory pathways 25,26 . A previous analysis of triarabinoside structures on the Arabidopsis CLV3 peptide showed that the Hyp residue was modified by three L-arabinose moieties with a linear b-1,2-linkage 26 . As [Ara 3 ]CLE-RS2 is present in plants at too low a level to obtain sufficient quantities for functional analyses, we decided to chemically synthesize [Ara 3 ]CLE-RS2.…”

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Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

et al. 2013

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Leguminous plants establish a symbiosis with rhizobia to enable nitrogen fixation in root nodules under the control of the presumed root-to-shoot-to-root negative feedback called autoregulation of nodulation. In Lotus japonicus, autoregulation is mediated by CLE-RS genes that are specifically expressed in the root, and the receptor kinase HAR1 that functions in the shoot. However, the mature functional structures of CLE-RS gene products and the molecular nature of CLE-RS/HAR1 signalling governed by these spatially distant components remain elusive. Here we show that CLE-RS2 is a post-translationally arabinosylated glycopeptide derived from the CLE domain. Chemically synthesized CLE-RS glycopeptides cause significant suppression of nodulation and directly bind to HAR1 in an arabinose-chain and sequencedependent manner. In addition, CLE-RS2 glycopeptide specifically produced in the root is found in xylem sap collected from the shoot. We propose that CLE-RS glycopeptides are the long sought mobile signals responsible for the initial step of autoregulation of nodulation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

et al. 2013

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“…5) (Kondo et al 2006;DeYoung and Clark 2008;Miwa et al 2008;Müller et al 2008;Ohyama et al 2009;Guo et al 2010;Kinoshita et al 2010;Betsuyaku et al 2011;Lee et al 2011). Among these receptors, the LRR domains for ligand perception or the PK domains for cellular signaling are more closely related between CLV1 and FLS2 than between CLV1 and CLV2 or RPK2 (Fig.…”

Section: Common Origins Of Receptor Kinases In Immunity and Developmentmentioning

confidence: 99%

Stem Cell Signaling in Immunity and Development

Lee

Chah

Plotnikov

et al. 2012

Cold Spring Harbor Symposia on Quantitative Biology

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Stem cells in the shoot apical meristem (SAM) of plants are the self-renewable reservoir for leaf, stem, and flower organogenesis. Stem-cell fate and population size are subject to regulation by complex intrinsic signals and environmental cues to ensure balanced plant development, survival, and longevity. Peptides secreted from the shoot stem cells have pivotal roles in controlling cell identity, proliferation, and differentiation through multiple receptor kinase complexes. The best-characterized in vivo and in vitro peptide ligands are the 12-amino acid (aa) and the arabinosylated 13-aa CLAVATA3 peptides (CLV3p) that are perceived by multiple receptors with partially overlapping and distinct expression patterns and functions in the SAM. The primary molecular and cellular signaling mechanisms after the occurrence of ligand-receptor interaction remain elusive. Integrated analyses provide novel evidence for differential peptide-receptor signaling in the dynamic regulation of stem-cell homeostasis and fitness. Surprisingly, the 12-aa CLV3p can trigger immune signaling and limit pathogen invasion via the flagellin receptor kinase FLS2, suggesting a previously unrecognized molecular mechanism underlying enhanced immunity in the SAM area. Because pattern recognition receptor signaling in immune responses also profoundly intercepts plant development, peptide-receptor kinase signaling in immunity and development may share a common evolutionary origin.

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“…CLV1 is the major receptor-like kinase for CLV3 signaling and is shown to interact specifically with CLV3 in vitro. [1][2][3] CLV2, a receptor-like protein, associates with CORYNE (CRN)/SUPPRESSOR OF LLP1 2 (SOL2), a membrane associated protein pseudo-kinase with a short extracellular domain. [4][5][6] RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) is another receptor-like kinase that is essential for CLV3 signaling.…”

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confidence: 99%