A glycolipid-specific monoclonal antibody modulates Fcϵ receptor stimulation of mast cells

Ortega, Enrique; Licht, Arieh; Biener, Yael; Pecht, Israel

doi:10.1016/0161-5890(90)90031-t

Cited by 16 publications

(10 citation statements)

References 18 publications

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“…The protocol employed for selection of mouse mAb specific for RBL-2H3 cell membrane components was based on the capacity of the mAb to modulate the Fc 4 RIinduced secretory response [5,7,25]. From four different hybridizations 156 clones were obtained producing mAb capable of inducing secretory response.…”

Section: Production Of Mab 2b5mentioning

confidence: 99%

An IgE-dependent secretory response of mast cells can be induced by a glycosphingolipid-specific monoclonal antibody

et al. 2000

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Section: Production Of Mab 2b5mentioning

confidence: 99%

An IgE-dependent secretory response of mast cells can be induced by a glycosphingolipid-specific monoclonal antibody

et al. 2000

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“…The following MAbs were used in this study: A2B5 (Boehringer-Mannheim; Mannheim, Germany), which reacts with a variety of polysialo-gangliosides (see Schwarz and Futerman 1996); B17 (IgM subclass) (provided by Dr. Israel Pecht, Department of Immunology, Weizmann Institute of Science), which reacts with ganglioside GD1b (Harel and Futerman 1993;Ortega et al 1990); JONES (IgM subclass) (provided by Dr. C.J. Barnstable, Yale University Medical School), which reacts with 9-O -acetylated-GD3 (Blum and Barnstable 1987;Constantine-Paton et al 1986); BMA190 (Behring Werke; Marburg, Germany), which reacts with GD2; R24 (IgG subclass) (Matreya), which reacts with GD3 (Pukel et al 1982;Dippold et al 1980).…”

Section: Antibodiesmentioning

confidence: 99%

Determination of the Localization of Gangliosides Using Anti-ganglioside Antibodies: Comparison of Fixation Methods

Schwarz

Futerman

1997

J Histochem Cytochem.

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SUMMARYMany studies have examined the localization of gangliosides using anti-ganglioside antibodies, although widely differing conclusions have been reached. We now demonstrate that the apparent localization of gangliosides can be greatly influenced by the fixation method. Using monoclonal antibody (MAb) A2B5 (which reacts with a variety of gangliosides), hippocampal neurons were labeled at the cell surface when incubated with the antibody before fixation, but when incubated after fixation the cells displayed a variety of labeling patterns, depending on the fixation method. Biochemical analysis demonstrated that some of the fixatives (particularly acetone and methanol) significantly reduced or completely depleted cellular gangliosides, implying that the immunoreactivity observed with A2B5, and with other antibodies, was not due to gangliosides. When neurons were incubated with an anti-GD1b antibody prefixation, uniform labeling of the plasma membrane was observed, but after ganglioside depletion using biochemical inhibitors of ganglioside synthesis no cell surface labeling was detected. However, even in cells depleted of gangliosides, labeling of both the cell surface and intracellular compartments was observed when the anti-GD1b antibody was applied after fixation. Moreover, after fixation, antibodies to GM4 and GD2 reacted with hippocampal neurons, although these gangliosides are absent from these neurons. In contrast, the JONES antibody (which reacts with 9-O -acetylated GD3) labeled neurons with a similar pattern, essentially irrespective of the fixation method. These observations demonstrate that great care must be taken in assigning gangliosides to specific cell populations or to intracellular locations solely on the basis of use of anti-ganglioside antibodies, and suggest that optimal fixation conditions must be established for each anti-ganglioside antibody. (J Histochem Cytochem 45:611-618, 1997)

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“…Finally, the distribution of a 'b-series' ganglioside, GD1b, was examined in 10 day old neurons using an anti-GD1b antibody [15]. Similar to younger neurons [8], GD1b was detected on both axons and dendrites (Fig.…”

Section: Resultsmentioning

confidence: 99%

Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons

et al. 1999

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Analysis of the binding of cholera toxin to ganglioside GM1 in both living and fixed neurons, and comparison with the distribution of defined axonal and dendritic proteins, demonstrates that ganglioside GM1 is distributed in a non-polarized manner over the axonal and dendritic plasma membranes of mature, cultured hippocampal neurons. Likewise, ganglioside GD1b is also distributed in a non-polarized manner. These results suggest that a recent report [Ledesma, M.D. et al. EMBO J. 18 (1999 proposing that ganglioside GM1 is highly enriched on the axonal versus dendritic membrane of hippocampal neurons may need to be re-evaluated.z 1999 Federation of European Biochemical Societies.

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A glycolipid-specific monoclonal antibody modulates Fcϵ receptor stimulation of mast cells

Cited by 16 publications

References 18 publications

An IgE-dependent secretory response of mast cells can be induced by a glycosphingolipid-specific monoclonal antibody

An IgE-dependent secretory response of mast cells can be induced by a glycosphingolipid-specific monoclonal antibody

Determination of the Localization of Gangliosides Using Anti-ganglioside Antibodies: Comparison of Fixation Methods

Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons

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