A Gly65Val substitution in an actin, GhACT_LI1, disrupts cell polarity and F‐actin organization resulting in dwarf, lintless cotton plants

Thyssen, Gregory N.; Fang, David D.; Turley, Rickie B.; Florane, Christopher B.; Li, Ping; Mattison, Christopher P.; Naoumkina, Marina

doi:10.1111/tpj.13477

Cited by 48 publications

(45 citation statements)

References 64 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, several Mendelian genes related to growth, disease resistance, and fiber development have been successfully identified in cotton. For instance, GhACT_LI1 [43], GoPGF [44], GhLMI1-D1b [45], GoSP [46], and GhLMMD [47] for fiber development, glandless phenotype, leaf shape, branching, and necrotic leaf damage have been cloned using the corresponding mutants, respectively.…”

Section: Map-based Cloningmentioning

confidence: 99%

Gossypium Genomics: Trends, Scope, and Utilization for Cotton Improvement

Yang

Qanmber

Wang

et al. 2020

Trends in Plant Science

111

View full text Add to dashboard Cite

Cotton (Gossypium spp.) is the most important natural fiber crop worldwide. The diversity of Gossypium species also provides an ideal model for investigating evolution and domestication of polyploids. However, the huge and complex cotton genome hinders genomic research. Technical advances in high-throughput sequencing and bioinformatics analysis have now largely overcome these obstacles, bringing about a new era of cotton genomics. Here, we review recent progress in Gossypium genomics based on whole genome sequencing, resequencing, and comparative genomics, which have provided insights about the genomic basis of fiber biogenesis and the landscape of cotton functional genomics. We address current challenges and present multidisciplinary genomics-enabled breeding strategies covering the breadth of high fiber yield, quality, and environmental resilience for future cotton breeding programs. Gossypium Genomics at a Glance As the world's most important fiber crop and a major source of seed oil and protein, cotton is cultivated in more than 75 countries around the globe [1]. Cotton fibers are seed trichomes, up to 65 mm long, composed of almost pure cellulose, and provide a unique single-celled model system for studying cell elongation and cell wall biogenesis [2]. The Gossypium genus is extraordinarily diverse, with eight diploid genome groups (A-G, and K) and one allopolyploid group (AD) [3,4]. Hybridization and polyploidization of two parental diploids, an A genome-like species with a D genome-like species, has resulted in at least seven allotetraploid species. Two allotetraploid species, Gossypium hirsutum and Gossypium barbadense, evolved independently; these two account for over 90% of annual commercial fiber production. Gossypium is ideal for investigating the origin, evolution, and domestication of polyploids [5-7]. Highlights Cotton is an important natural fiber crop cultivated worldwide that also provides an ideal model for investigating evolution and domestication of polyploids Combinations of the latest technologies, such as optical mapping, high-throughput chromosome conformation capture (Hi-C), and Pacific Biosciences (PacBio) long-reads, have been used to generate multiple high-quality reference genomes of diploid and allotetraploid cotton. Comparative population genomics illuminated the genetic history of cotton domestication and identified the genomic variation determining fiber yield, quality, and stress resistance.

show abstract

Section: Map-based Cloningmentioning

confidence: 99%

Gossypium Genomics: Trends, Scope, and Utilization for Cotton Improvement

Yang

Qanmber

Wang

et al. 2020

Trends in Plant Science

111

View full text Add to dashboard Cite

show abstract

“…Fuzz fibers are usually <5 mm in length and remain attached to the seeds after ginning (Zhang et al, 2007;Mauney, 2015). Currently, numerous naturally occurring cotton fiber mutants have been identified globally and characterized at the genetic, gene expression, and, more recently, genetic mapping (map-based cloning) levels (Rong et al, 2005;Hinchliffe et al, 2011;Kim et al, 2013a;Wang et al, 2014a;Naoumkina et al, 2015;Hu et al, 2016;Wan et al, 2016;Thyssen et al, 2017;Zhu et al, 2018). It is worth noting that it is difficult and often unpractical to differentiate fuzz fibers from extremely short (<6 mm) lint fibers before or after ginning.…”

Section: Unraveling Cotton Fiber Development Using Fiber Mutants In Tmentioning

confidence: 99%

“…Complementary DNA microarray and candidate gene analysis based on the genome sequences of the diploid G. raimondii identified a handful of candidates including an actin near the Li 1 locus (Gilbert et al, 2014;Jiang et al, 2015). Using high-resolution genetic mapping, Thyssen et al (2017) reported that the causative gene of the Li 1 mutation is a substitution of glycine to valine at position 65 in the protein sequence of an actin gene, GhACT_LI1 (Gh_D04G0865). This alteration disrupts progressive elongation of F-actin, resulting in a disorganized cytoskeleton and reduced cell polarity, and consequently dwarf cotton plants with very short fibers.…”

Section: Actins and Annexins And Their Roles In Fiber Elongationmentioning

confidence: 99%

“…A few more mutants were discovered later (Ware et al, 1947;Kohel et al, 1974;Kohel et al, 1992). Currently, numerous naturally occurring cotton fiber mutants have been identified globally and characterized at the genetic, gene expression, and, more recently, genetic mapping (map-based cloning) levels (Rong et al, 2005;Hinchliffe et al, 2011;Kim et al, 2013a;Wang et al, 2014a;Naoumkina et al, 2015;Hu et al, 2016;Wan et al, 2016;Thyssen et al, 2017;Zhu et al, 2018). In addition, man-made fiber mutants were developed through chemical mutagenesis (Bechere et al, 2012;Naoumkina et al, 2017a) and the selection of a tissue culture-induced somatic mutation (Cai et al, 2013).…”

Section: Unraveling Cotton Fiber Development Usingmentioning

confidence: 99%

See 1 more Smart Citation

Unraveling Cotton Fiber Development Using Fiber Mutants in the Post‐Genomic Era

2018

Self Cite

View full text Add to dashboard Cite

Cotton (Gossypium spp.) fibers are unicellular trichomes that differentiate from ovule epidermal cells. Cotton fiber development is divided into four distinct yet overlapping stages: initiation, elongation, secondary cell wall (SCW) biosynthesis, and maturation. There are numerous naturally occurring and man‐made fiber mutants that display aberrant phenotypes ranging from fiberless to extremely short fiber, and to immature fiber. These mutants have provided cotton researchers an excellent model system to study fiber growth and development. During the past two decades, much advancement in the understanding of fiber development has been achieved through comparative analyses of fiber mutants and wild‐type cotton lines using a variety of technologies such as transcriptomic analysis and genetic mapping. The causative genes of five mutations related to fiber initiation, elongation, or SCW assembly have been identified. Lint and fuzz fiber initiations are regulated by MYBMIXTA‐like transcription factors along with many other genes. Cytoskeleton actins play a critical role in fiber elongation. Genes that are involved in biological processes such as transporting osmoticum or loosening cell walls are highly expressed during the elongation stage. Production of large amounts of cellulose at the SCW stage requires a sustainable supply of energy and carbohydrate precursors. Plant hormones affect fiber development. In this paper, we review progress in the elucidation of fiber development mechanisms based on analyzing fiber mutants and summarize genes and mechanisms that are critical to fiber development. We identify research gaps that should be future research priorities and provide a future perspective.

show abstract

“…The plant actin cytoskeleton plays crucial roles in diverse cellular processes, such as cell elongation, cell division, intracellular transportation and cell wall biosynthesis (Liu et al ). Growing evidence demonstrates that cell elongation and secondary wall synthesis are associated with the dynamic reorganization of both actin cytoskeleton and microtubules in developing cotton fibers (Quader et al ; Seagull ; Wang et al ; Han et al ; Thyssen et al ). A number of cytoskeletal genes were found to express preferentially in fiber cells, and some of them were shown to function in the regulation of fiber development (Li et al ; Wang et al ; Wang et al ; Han et al ; Zhang et al ).…”

mentioning

confidence: 99%

Overexpression of GhFIM2 propels cotton fiber development by enhancing actin bundle formation

Zhang

Han

Wang

et al. 2017

JIPB

View full text Add to dashboard Cite

Summary Cell elongation and secondary wall deposition are two consecutive stages during cotton fiber development. The mechanisms controlling the progression of these two developmental phases remain largely unknown. Here, we report the functional characterization of the actin‐bundling protein GhFIM2 in cotton fiber. Overexpression of GhFIM2 increased the abundance of actin bundles, which was accompanied with accelerated fiber growth at the fast‐elongating stage. Meanwhile, overexpression of GhFIM2 could propel the onset of secondary cell wall biogenesis. These results indicate that the dynamic rearrangement of actin higher structures involving GhFIM2 plays an important role in the development of cotton fiber cells.

show abstract

A Gly65Val substitution in an actin, GhACT_LI1, disrupts cell polarity and F‐actin organization resulting in dwarf, lintless cotton plants

Cited by 48 publications

References 64 publications

Gossypium Genomics: Trends, Scope, and Utilization for Cotton Improvement

Gossypium Genomics: Trends, Scope, and Utilization for Cotton Improvement

Unraveling Cotton Fiber Development Using Fiber Mutants in the Post‐Genomic Era

Overexpression of GhFIM2 propels cotton fiber development by enhancing actin bundle formation

Contact Info

Product

Resources

About