A glue-based, screw-free method for implantation of intra-cranial electrodes in young mice

Wu, Chenggang; Wais, Marta; Sheppy, Evan; Campo, Martín del; Zhang, Liang

doi:10.1016/j.jneumeth.2008.03.001

Cited by 40 publications

(44 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the glue-based method is suitable for longterm intrahippocampal and cortical EEG recordings (Wu et al, 2008), its utilization in other experimental conditions remains untested. For example, it may be difficult to attach an electrode array to curved skull regions.…”

Section: Resultsmentioning

confidence: 99%

“…Surgical procedures were modified from those previously described (in Wu et al, 2008). Mice 25-35 days (n 7) or 13-19 months (n 5) of age were operated on for electrode implantation.…”

Section: Methodsmentioning

confidence: 99%

“…To minimize the adverse effects that are potentially associated with the use of anchoring screws, we have developed a screw-free, glue-based method for implantation of intracranial electrodes in mice (Wu, Wais, Sheppy, del Campo, & Zhang, 2008). Our approach is to construct an electrode array, which consists of a plastic base and three assembled electrodes that are arranged according to the stereotaxic coordinates of hippocampal and neocortical recording sites.…”

mentioning

confidence: 99%

“…The headstages were placed near the animal-housing cage (approximately 10 cm) and were connected to implanted electrodes using soft wires and female pins (Wu et al, 2008). The signals were recorded in the frequency band of 0.05-1000 Hz and were amplified 1,000 times before digitization (digitization rates of 20 or 60 KHz; Digidata 1300, Molecular Devices, Union City, CA).…”

mentioning

confidence: 99%

See 3 more Smart Citations

An improved screw-free method for electrode implantation and intracranial electroencephalographic recordings in mice

Wais

Zahid

et al. 2009

Behavior Research Methods

Self Cite

View full text Add to dashboard Cite

We recently developed a glue-based method for the implantation of intracranial electrodes in mice. Our approach is to secure a preconstructed electrode array using a cyanoacrylate-based glue (similar to Krazy Glue). This method is applicable to both young and aging mice and is suitable for long-term electroencephalographic recordings. In the present experiment, we explored whether the glue-based method is capable of securing individual electrodes in addition to securing the electrode array. C57 black mice aged 25-35 days or 13-19 months were operated on under isoflurane anesthesia. Monopolar or bipolar electrodes were inserted independently in the ipsilateral hippocampal CA3 and entorhinal cortical areas, and they were fixed onto the skull using the glue together with dental acrylic, but without anchoring screws. We found that the implanted electrodes were stable and allowed repeat intracranial recordings and electrical stimulation in freely moving mice.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Surgical procedures were modified from those previously described (in Wu et al, 2008). Mice 25-35 days (n 7) or 13-19 months (n 5) of age were operated on for electrode implantation.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

An improved screw-free method for electrode implantation and intracranial electroencephalographic recordings in mice

Wais

Zahid

et al. 2009

Behavior Research Methods

Self Cite

View full text Add to dashboard Cite

show abstract

“…Experimental and control animals were implanted with polyimide-insulated stainless steel microelectrodes (125 μm) in somatosensory cortex (Bregma, − 0.8 mm; lateral, 1.8 mm; depth, 1.5 mm) following the procedures outlined previously (Wu et al, 2008;D'Cruz et al, 2010;Lang et al, 2013). The reference electrode was implanted superficially into the frontal brain area (Bregma, +2.8 mm; lateral, 1.8 mm; depth, 0.5 mm).…”

Section: Implantation Surgerymentioning

confidence: 99%

A Role for Diminished GABA Transporter Activity in the Cortical Discharge Phenotype of MeCP2-Deficient Mice

Zhang

Wither

Lang

et al. 2015

Neuropsychopharmacol

Self Cite

View full text Add to dashboard Cite

Cortical network hyper-excitability is a common phenotype in mouse models lacking the transcriptional regulator methyl-CPG-binding protein 2 (MeCP2). Here, we implicate enhanced GABA B receptor activity stemming from diminished cortical expression of the GABA transporter GAT-1 in the genesis of this network hyper-excitability. We found that administering the activity-dependent GABA B receptor allosteric modulator GS-39783 to female Mecp2 +/ − mice at doses producing no effect in wild-type mice strongly potentiated their basal rates of spontaneous cortical discharge activity. Consistently, administering the GABA B receptor antagonist CGP-35348 significantly decreased basal discharge activity in these mice. Expression analysis revealed that while GABA B or extra-synaptic GABA A receptor prevalence is preserved in the MeCP2-deficient cortex, the expression of GAT-1 is significantly reduced from wild-type levels. This decrease in GAT-1 expression is consequential, as low doses of the GAT-1 inhibitor NO-711 that had no effects in wild-type mice strongly exacerbated cortical discharge activity in female Mecp2 +/ − mice. Taken together, these data indicate that the absence of MeCP2 leads to decreased cortical levels of the GAT-1 GABA transporter, which facilitates cortical network hyper-excitability in MeCP2-deficient mice by increasing the activity of cortical GABA B receptors.

show abstract

EEG Recording in Rodents, with a Focus on Epilepsy

2009

View full text Add to dashboard Cite

show abstract

A glue-based, screw-free method for implantation of intra-cranial electrodes in young mice

Cited by 40 publications

References 8 publications

An improved screw-free method for electrode implantation and intracranial electroencephalographic recordings in mice

An improved screw-free method for electrode implantation and intracranial electroencephalographic recordings in mice

A Role for Diminished GABA Transporter Activity in the Cortical Discharge Phenotype of MeCP2-Deficient Mice

EEG Recording in Rodents, with a Focus on Epilepsy

Contact Info

Product

Resources

About