a-Glucosidase Activity in the Reproductive Tract of the Ewe

O’Shea, Tim; Murdoch, BE

doi:10.1071/bi9780363

Cited by 4 publications

(2 citation statements)

References 12 publications

(17 reference statements)

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“…In this tissue IX-amylase and glycogen phosphorylase remained constant, but the activity of acid lX-l,4-g1ucosidase was at a maximum at day 0 in the cervical mucosa (O'Shea and Murdoch 1978b). Thus in the oviduct and cervix both synthesis and degradation of glycogen are increased at oestrus.…”

Section: Discussionmentioning

confidence: 75%

Activity of Enzymes in the Mucosal Tissues and Rinsings of the Reproductive Tract of the Naturally Cyclic Ewe

Murdoch¹,

O’Shea²

1978

Aust. Jnl. Of Bio. Sci.

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The activity of several enzymes has been measured in the oviducal mucosa, endometrium, caruncles, cervical mucosa and vaginal mucosa as well as in uterine, cervical and oviducal rinsings of ewes at days 0 (oestrus), 1, 8 and 15 of the oestrous cycle.Acid phosphatase activity was maximal during the luteal phase in the oviduct, endometrium and cervix, but not in the vagina where the highest level was on day 1. Alkaline phosphatase activity showed a significant rise at mid-cycle in the endometrium and caruncles as did glucose-6-phosphate dehydrogenase activity in the endometrium.Glycogen (starch) synthase in the endometrium and cervical mucosa was greater at days 0 and 8 than at days 1 and 15. Vaginal and caruncular total glycogen (starch) synthase activity showed a significant rise at mid-cycle. Active and total glycogen phosphorylase activities were maximal on days 8 and 15 in the vagina. Glycogen concentration was greatest at days 8 and 15 in the cervix, while oviduct values were lower on day 1 than at oestrus or day 15.In the oviduct rinsings alkaline phosphatase activity was greatest at day 1, whereas in cervical rinsings the activities of acid and alkaline phosphatases were less at oestrus than at days 8 and 15. The glycogen phosphorylase and p-glucuronidase activities in uterine rinsings were greater at days 8 and 15 than at oestrus.

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Section: Discussionmentioning

confidence: 75%

Activity of Enzymes in the Mucosal Tissues and Rinsings of the Reproductive Tract of the Naturally Cyclic Ewe

Murdoch¹,

O’Shea²

1978

Aust. Jnl. Of Bio. Sci.

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“…A study in ewes determined that GAA activity did not change through the oestrous cycle (O'Shea & Murdoch, 1978). In agreement, we found that the activity of GAA did not increase in BUTE cells treated with progesterone.…”

Section: Discussionmentioning

confidence: 99%

The glycogenolytic enzyme acid α‐glucosidase is expressed in the bovine uterine endometrium

Berg,

Dean

2024

Reprod Domestic Animals

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Progesterone has been shown to stimulate glycogen catabolism in uterine epithelial cells. Acid α‐glucosidase (GAA) is an enzyme that breaks down glycogen within lysosomes. We hypothesized that progesterone may stimulate glycogenolysis in the uterine epithelium via GAA. We found that GAA was more highly expressed in the stroma on Day 1 than on Day 11. However, GAA did not appear to differ in the epithelium on Days 1 and 11. Progesterone (0‐10 μM) had no effect on the levels of the full‐length inactive protein (110 kDa) or the cleaved (active) peptides present inside the lysosome (70 and 76 kDa) in immortalized bovine uterine epithelial (BUTE) cells. Furthermore, the activity of GAA did not differ between the BUTE cells treated with 10 μM progesterone or control. Overall, we confirmed that GAA is present in the cow endometrium and BUTE cells. However, progesterone did not affect protein levels or enzyme activity.

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Effect of equine chorionic gonadotropin on glycosidase activity in the reproductive tract of ewes, in relation to ovarian response and embryo yield

Samartzi

Theodosiadou

Rekkas

et al. 2020

Small Ruminant Research

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a-Glucosidase Activity in the Reproductive Tract of the Ewe

Cited by 4 publications

References 12 publications

Activity of Enzymes in the Mucosal Tissues and Rinsings of the Reproductive Tract of the Naturally Cyclic Ewe

Activity of Enzymes in the Mucosal Tissues and Rinsings of the Reproductive Tract of the Naturally Cyclic Ewe

The glycogenolytic enzyme acid α‐glucosidase is expressed in the bovine uterine endometrium

Effect of equine chorionic gonadotropin on glycosidase activity in the reproductive tract of ewes, in relation to ovarian response and embryo yield

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