A Glucose Sensor Fabricated by Piezoelectric Inkjet Printing of Conducting Polymers and Bienzymes

Yun, Yeon Hee; Lee, Byung Kook; Choi, Ji Suk; Kim, Sung-Won; Yoo, Bongyoung; Kim, Yong Shin; Park, Kinam; Cho, Yong Woo

doi:10.2116/analsci.27.375

Cited by 57 publications

(23 citation statements)

References 32 publications

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“…This may be favorable with the use of enzyme and dye solutions as it prevents the potential enzyme denaturation that heat could cause. 7 However, it should be noted that several thermal inkjet printers have been found to have minimal effect on protein denaturation. 8,9 The strip has been designed to provide calibration with each test.…”

Section: Methodsmentioning

confidence: 99%

A Low-Cost Inkjet-Printed Glucose Test Strip System for Resource-Poor Settings

Wilson

Ovington

Dean

2015

J Diabetes Sci Technol

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Background: The prevalence of diabetes is increasing in low-resource settings; however, accessing glucose monitoring is extremely difficult and expensive in these regions. Work is being done to address the multitude of issues surrounding diabetes care in low-resource settings, but an affordable glucose monitoring solution has yet to be presented. An inkjetprinted test strip solution is being proposed as a solution to this problem. Methods:The use of a standard inkjet printer is being proposed as a manufacturing method for low-cost glucose monitoring test strips. The printer cartridges are filled with enzyme and dye solutions that are printed onto filter paper. The result is a colorimetric strip that turns a blue/green color in the presence of blood glucose.Results: Using a light-based spectroscopic reading, the strips show a linear color change with an R 2 = .99 using glucose standards and an R 2 = .93 with bovine blood. Initial testing with bovine blood indicates that the strip accuracy is comparable to the International Organization for Standardization (ISO) standard 15197 for glucose testing in the 0-350 mg/dL range. However, further testing with human blood will be required to confirm this. A visible color gradient was observed with both the glucose standard and bovine blood experiment, which could be used as a visual indicator in cases where an electronic glucose meter was unavailable. Conclusions:These results indicate that an inkjet-printed filter paper test strip is a feasible method for monitoring blood glucose levels. The use of inkjet printers would allow for local manufacturing to increase supply in remote regions. This system has the potential to address the dire need for glucose monitoring in low-resource settings.

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Section: Methodsmentioning

confidence: 99%

A Low-Cost Inkjet-Printed Glucose Test Strip System for Resource-Poor Settings

Wilson

Ovington

Dean

2015

J Diabetes Sci Technol

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“…The use of wax for the creation of microfluidic devices has been used to create paper and glass-based devices as a simple and inexpensive method using commercially available materials [23][24][25] . This ability to create biosensors has been investigated to an extent with inkjet printing technology [26][27][28][29] . In this paper we have described for first time inkjet printing paraffin wax on tissue culture plastic and on a plain glass substrate and these formed structures were then used to guide cell attachment, spreading and proliferation, without further processing steps.…”

Section: Introductionmentioning

confidence: 99%

Utilising inkjet printed paraffin wax for cell patterning applications

Tse

Stringer

et al. 2024

IJB

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Abstract:We describe a method to prepare patterned environments for eukaryotic cells by inkjet printing paraffin wax onto a substrate. This technique bypasses the requirement to create a master mould, typically required with the use of polydimethylsiloxane techniques and the printed structure could be immediately used to guide cell proliferation. In a space of 2-3 hours, the desired pattern could be created with computer assisted design, printed and have cells seeded onto the scaffold, which could reduce the cycle time of prototyping micropattern designs. Human dermal fibroblasts and RN22 Schwann cells were seen to proliferate within the fabricated patterns and survive for more than 7 days. Additionally, the wax constructs could be readily removed from the substrate at any stage after cell seeding with the cells continuing to proliferate. Thus, we report on a simple but novel approach for the controlled physical positioning of live cells by wax inkjet printing.

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“…Inkjet printing confers more advantages: in the field of nanotechnologies, it has been exploited to produce a formulation capable of controlling the release of a drug [20], to better functionalize biosensors [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Nanoprobe NAPPA Arrays for the Nanoconductimetric Analysis of Ultra-Low-Volume Protein Samples Using Piezoelectric Liquid Dispensing Technology

Pechkova¹,

Wiktor²,

Bragazzi³

et al. 2015

NanoWorld J

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In the last years, the evolution and the advances of the nanobiotechnologies applied to the systematic study of proteins, namely proteomics, both structural and functional, and specifically the development of more sophisticated and largescale protein arrays, have enabled scientists to investigate protein interactions and functions with an unforeseeable precision and wealth of details. Here, we present a further advancement of our previously introduced and described Nucleic Acid Programmable Protein Arrays (NAPPA)-based nanoconductometric sensor. We coupled Quartz Crystal Microbalance with Dissipation factor Monitoring (QCM_D) with piezoelectric inkjet printing technology (namely, the newly developed ActivePipette), which enables to significantly reduce the volume of probe required for genes/proteins arrays. We performed a negative control (with master mix, or MM) and a positive control (MM_p53 plus MDM2). We performed this experiment both in static and in flow, computing the apparent dissociation constant of p53-MDM2 complex (130 nM, in excellent agreement with the published literature). We compared the results obtained with the ActivePipette printing and dispensing technology vs. pin spotting. Without the ActivePipette, after MDM2 addition the shift in frequency (Δf ) was 7575 Hz and the corresponding adsorbed mass was 32.9 µg. With the ActivePipette technology, after MDM2 addition Δf was 7740 Hz and the corresponding adsorbed mass was 33.6 µg. With this experiment, we confirmed the sensing potential of our device, being able to discriminate each gene and protein as well as their interactions, showing for each one of them a unique conductance curve. Moreover, we obtained a better yield with the ActivePipette technology.

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A Glucose Sensor Fabricated by Piezoelectric Inkjet Printing of Conducting Polymers and Bienzymes

Cited by 57 publications

References 32 publications

A Low-Cost Inkjet-Printed Glucose Test Strip System for Resource-Poor Settings

A Low-Cost Inkjet-Printed Glucose Test Strip System for Resource-Poor Settings

Utilising inkjet printed paraffin wax for cell patterning applications

Nanoprobe NAPPA Arrays for the Nanoconductimetric Analysis of Ultra-Low-Volume Protein Samples Using Piezoelectric Liquid Dispensing Technology

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