A glance on the role of fibronectin in controlling cell response at biomaterial interface

Abstract

SummaryThe bioactivity of biomaterials is closely related to cell response in contact with them. However, shortly after their insertion, materials are soon covered with proteins that constitute the biological fluids, and which render the direct surface recognition by cells almost impossible. The control of protein adsorption at the interface is therefore desirable. Extracellular matrix proteins are of particular interest in this sense, due to their well-known ability to modulate cell behavior. Particularly, fibronectin plays a leading role, being present in both healthy and injured tissues undergoing healing and regeneration. The aim of the present work is to give an overview on fibronectin and on its involvement in the control of cell behavior providing evidence of its pivotal role in the control of cell adhesion, spreading, migration, proliferation and differentiation. A deep insight into methods to enrich biomaterials surface with fibronectin will be then discussed, as well as new cues on the possibility to design tailored platforms able to specifically retain fibronectin from the surrounding extracellular milieu.

Regulation of cell locomotion by nanosecond-laser-induced hydroxyapatite patterning

“…When an implant material is placed in the body, the serum proteins first contact the material surface [ 39 ], and the amount and type of attached serum proteins are known to affect the adhesion and differentiation of bone-forming cells and bone formation [ 40 , 41 ]. In particular, fibronectin (FN) [ 42 , 43 ] and vitronectin (VN) [ 44 , 45 ] are known to promote the formation of focal adhesions of osteoblasts, resulting in favorable adhesion, proliferation, and differentiation of osteoblasts and ultimately bone formation [ [46] , [47] , [48] , [49] ].…”

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

“…In the case of fibronectin derived sequences, the PHSRN (Pro-His-Ser-Arg-Asn), REDV (Arg-Glu-Asp-Val), LDV (Leu-Asp-Val), and KQAGDV (Lys-Gln-Ala-Gly-Asp-Val) are usually used in TE. Similar to collagen-derived sequences, they also possess the ability to bind to integrin receptors resulting in enhanced adhesion and proliferation of many cells, such as fibroblasts, MSCs, and endothelial cells [38,[120][121][122][123].…”

Fibronectin in Fracture Healing: Biological Mechanisms and Regenerative Avenues

“…receptors can, in part, direct cytoskeletal arrangements, which stretch the FN molecules and promote a positive feedback loop of fibril formation (Antia et al, 2008). Integrin α5ß1 expressed on osteogenic cells and fibroblasts, along with αVß3 expressed on osteogenic cells and ECs, seem to play a crucial role in mediating cellular attachment to bone ECM (Parisi et al, 2019b).…”