Abstract:Urinary and intravascular catheters
are two of the most used invasive
medical devices; however, microbial colonization of catheter surfaces
is responsible for most healthcare-associated infections (HAIs). Several
antimicrobial-coated catheters are available, but recurrent antibiotic
therapy can decrease their potential activity against resistant bacterial
strains. The aim of this Review is to question the actual effectiveness
of currently used (coated) catheters and describe the progress and
promise of alterna… Show more
“…Moreover, for the interaction between BAC molecules and bacteria to occur, the particles need to be in close contact with bacteria. Of course, the BAC molecules inside the pores will not be readily available for bacteria [ 62 ], since the particles are mesoporous (pore diameters between 2 and 50 nm [ 63 ]) and bacteria (due to their bigger size) cannot enter the pores. In the work of He et al [ 56 ], they found that bacteria are dead upon contact with the immobilized QACs.…”
Reverse osmosis (RO) depends on biocidal agents to control the operating costs associated to biofouling, although this implies the discharge of undesired chemicals into the aquatic environment. Therefore, a system providing pre-treated water free of biocides arises as an interesting solution to minimize the discharge of chemicals while enhancing RO filtration performance by inactivating bacteria that could form biofilms on the membrane system. This work proposes a pretreatment approach based on the immobilization of an industrially used antimicrobial agent (benzalkonium chloride—BAC) into millimetric aluminum oxide particles with prior surface activation with DA—dopamine. The antimicrobial efficacy of the functionalized particles was assessed against Escherichia coli planktonic cells through culturability and cell membrane integrity analysis. The results showed total inactivation of bacterial cells within five min for the highest particle concentration and 100% of cell membrane damage after 15 min for all concentrations. When reusing the same particles, a higher contact time was needed to reach the total inactivation, possibly due to partial blocking of immobilized biocide by dead bacteria adhering to the particles and to the residual leaching of biocide. The overall results support the use of Al2O3-DA-BAC particles as antimicrobial agents for sustainable biocidal applications in continuous water treatment systems.
“…Moreover, for the interaction between BAC molecules and bacteria to occur, the particles need to be in close contact with bacteria. Of course, the BAC molecules inside the pores will not be readily available for bacteria [ 62 ], since the particles are mesoporous (pore diameters between 2 and 50 nm [ 63 ]) and bacteria (due to their bigger size) cannot enter the pores. In the work of He et al [ 56 ], they found that bacteria are dead upon contact with the immobilized QACs.…”
Reverse osmosis (RO) depends on biocidal agents to control the operating costs associated to biofouling, although this implies the discharge of undesired chemicals into the aquatic environment. Therefore, a system providing pre-treated water free of biocides arises as an interesting solution to minimize the discharge of chemicals while enhancing RO filtration performance by inactivating bacteria that could form biofilms on the membrane system. This work proposes a pretreatment approach based on the immobilization of an industrially used antimicrobial agent (benzalkonium chloride—BAC) into millimetric aluminum oxide particles with prior surface activation with DA—dopamine. The antimicrobial efficacy of the functionalized particles was assessed against Escherichia coli planktonic cells through culturability and cell membrane integrity analysis. The results showed total inactivation of bacterial cells within five min for the highest particle concentration and 100% of cell membrane damage after 15 min for all concentrations. When reusing the same particles, a higher contact time was needed to reach the total inactivation, possibly due to partial blocking of immobilized biocide by dead bacteria adhering to the particles and to the residual leaching of biocide. The overall results support the use of Al2O3-DA-BAC particles as antimicrobial agents for sustainable biocidal applications in continuous water treatment systems.
“…Extended spectrum β-lactamase (ESBL) and carbapenemase producing Gram negative bacteria have emerged as a recalcitrant therapeutic challenge. These MDR microbes are the most notorious group of bacteria that pose a serious global health threat [ 32 , 33 ]. We found that our antimicrobial biomaterials ( PDMS-PIM1 ) inhibited the growth of all the ESKAPE group of clinical isolates as well as MRSA and Vancomycin-resistant Enterococcus ( VRE ) pathogens (Fig.…”
Background
In addition to the widespread use of antibiotics in healthcare settings, the current COVID-19 pandemic has escalated the emergence of antibiotic resistance. Nosocomial infections among hospitalized patients is a leading site for such resistant microbial colonization due to prolonged use of invasive devices and antibiotics in therapies. Invasive medical devices, especially catheters, are prone to infections that could accelerate the development of resistant microbes. Often, catheters - particularly urinary catheters - are prone to high infection rates. Antibiotic-coated catheters can reduce infection rates and although commercially available, are limited in efficacy and choices.
Methods
Herein, a novel and facile method to fabricate PMDS-based biomaterial for the development of antimicrobial eluting catheters is presented. Silicone based organic polymer polydimethylsiloxane (PDMS) was used to prepare a biomaterial containing novel polymeric imidazolium antimicrobial compound.
Results
It was found that the PDMS-based biomaterials could eradicate microbial colonization even after 60 days in culture with continuous microbial challenge, be recycled over multiple uses, stored at room temperature for long-term usage and importantly is biocompatible.
Conclusion
The PDMS-based biomaterial displayed biocidal functionality on microbes of clinical origin, which form major threats in hospital acquired infections.
Graphical Abstract
“…One of the most clinically relevant aspects of biomaterial-related infection control is that of catheter-associated infections. As reviewed by Ricado et al , despite several decades of intensive research and development involving those strategies discussed in the previous sections, and others, efficacy data obtained from in vitro studies is not properly reflected in the clinical setting [ 133 ].…”
Section: Categories Of Bioactive Materialsmentioning
This essay analyzes the scientific evidence that forms the basis of bioactive materials, covering the fundamental understanding of bioactivity phenomena and correlation with the mechanisms of biocompatibility of biomaterials. This is a detailed assessment of performance in areas such as bone-induction, cell adhesion, immunomodulation, thrombogenicity and antimicrobial behavior. Bioactivity is the modulation of biological activity by characteristics of the interfacial region that incorporates the material surface and the immediate local host tissue. Although the term ‘bioactive material’ is widely used and has a well understood general meaning, it would be useful now to concentrate on this interfacial region, considered as ‘
the bioactivity zone’
. Bioactivity phenomena are either due to topographical/micromechanical characteristics, or to biologically active species that are presented in the bioactivity zone. Examples of topographical/micromechanical effects are the modulation of the osteoblast – osteoclast balance, nanotopographical regulation of cell adhesion, and bactericidal nanostructures. Regulation of bioactivity by biologically active species include their influence, especially of metal ions, on signaling pathways in bone formation, the role of cell adhesion molecules and bioactive peptides in cell attachment, macrophage polarization by immunoregulatory molecules and antimicrobial peptides. While much experimental data exists to demonstrate the potential of such phenomena, there are considerable barriers to their effective clinical translation. This essay shows that there is solid scientific evidence of the existence of bioactivity mechanisms that are associated with some types of biomaterials, especially when the material is modified in a manner designed to specifically induce that activity.
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