A ginseng PgTIP1 gene whose protein biological activity related to Ser128 residue confers faster growth and enhanced salt stress tolerance in Arabidopsis

Li, Jia; Cai, Weiming

doi:10.1016/j.plantsci.2015.02.001

Cited by 12 publications

(19 citation statements)

References 71 publications

(86 reference statements)

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“…Transgenic Arabidopsis plants expressing SlTIP2;2 from Solanum lycopersicum exhibited enhanced salt stress tolerance by regulating ion (including Na + and K + ) homeostasis and antioxidant enzyme activities under salt treatment (Xin et al, 2014). A high water channel activity was displayed by PgTIP1- heterologous expression in Xenopus laevis oocytes and in yeast, and a significant role in growth and development under favorable conditions and enhanced salt and drought tolerance, together with upregulated expression of the stress-related genes, was demonstrated in PgTIP1- transgenic Arabidopsis plants (Lin et al, 2007; Peng et al, 2007; Li and Cai, 2015). In our work, we successfully obtained PgTIP1 -transformed soybean cotyledon hairy roots and composite plants mediated by A. rhizogenes , and PgTIP1 -transgenic plants (lines N and J11) through the pollen tube pathway without a marker gene ( Figures 1A , 2A , 4B ).…”

Section: Discussionmentioning

confidence: 99%

“…It was also shown to have a significant role in the growth and development of PgTIP1- transgenic Arabidopsis plants under favorable conditions, including enhanced seed size and mass, and higher fatty acid content than the wild-type control (Lin et al, 2007; Li and Cai, 2015). Another related study by Peng et al (2007) reported that the overexpression of PgTIP1 in transgenic Arabidopsis plants led to enhanced tolerance against salt and drought stress, but lowered cold acclimation ability.…”

Section: Introductionmentioning

confidence: 99%

“…Another related study by Peng et al (2007) reported that the overexpression of PgTIP1 in transgenic Arabidopsis plants led to enhanced tolerance against salt and drought stress, but lowered cold acclimation ability. Further work by Li and Cai (2015) suggested that the conferred faster growth and enhanced salt tolerance in transgenic Arabidopsis plants was related to its water channel activity of the Ser 128 residue of PgTIP1 . Functional analyses using the plant PgTIP1- transgenic technique have predominantly focused on Xenopus laevis oocytes, yeast, or non-crop Arabidopsis , whereas related studies on crops have rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

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Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants

Che

et al. 2017

Front. Plant Sci.

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The Panax ginseng TIP gene PgTIP1 was previously demonstrated to have high water channel activity by its heterologous expression in Xenopus laevis oocytes and in yeast; it also plays a significant role in growth of PgTIP1-transgenic Arabidopsis plants under favorable conditions and has enhanced tolerance toward salt and drought treatment. In this work, we first investigated the physiological effects of heterologous PgTIP1 expression in soybean cotyledon hairy roots or composite plants mediated by Agrobacterium rhizogenes toward enhanced salt tolerance. The PgTIP1-transgenic soybean plants mediated by the pollen tube pathway, represented by the lines N and J11, were analyzed at the physiological and molecular levels for enhanced salt tolerance. The results showed that in terms of root-specific heterologous expression, the PgTIP1-transformed soybean cotyledon hairy roots or composite plants displayed superior salt tolerance compared to the empty vector-transformed ones according to the mitigatory effects of hairy root growth reduction, drop in leaf RWC, and rise in REL under salt stress. Additionally, declines in K+ content, increases in Na+ content and Na+/K+ ratios in the hairy roots, stems, or leaves were effectively alleviated by PgTIP1-transformation, particularly the stems and leaves of composite soybean plants. At the whole plant level, PgTIP1-trasgenic soybean lines were found to possess stronger root vigor, reduced root and leaf cell membrane damage, increased SOD, POD, CAT, and APX activities, steadily increased leaf Tr, RWC, and Pn values, and smaller declines in chlorophyll and carotenoid content when exposed to salt stress compared to wild type. Moreover, the distribution patterns of Na+, K+, and Cl- in the roots, stems, and leaves of salt-stressed transgenic plants were readjusted, in that the absorbed Na+ and Cl- were mainly restricted to the roots to reduce their transport to the shoots, and the transport of root-absorbed K+ to the shoots was simultaneously promoted. PgTIP1 transformation into soybean plants enhanced the expression of some stress-related genes (GmPOD, GmAPX1, GmSOS1, and GmCLC1) in the roots and leaves under salt treatment. This indicates that the causes of enhanced salt tolerance of heterologous PgTIP1-transformed soybean are associated with the positive regulation on water relations, ion homeostasis, and ROS scavenging under salt stress both at root-specific and whole plant levels.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants

Che

et al. 2017

Front. Plant Sci.

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Section: Introductionmentioning

confidence: 94%

“…Supporting the importance of these proteins in plant stress adaptation, TIPs were shown to improve drought and salt tolerance when overexpressed in transgenic plants (Peng et al, 2007; Khan et al, 2015; Li and Cai, 2015). Intriguingly, TIP overexpression also positively affected plant growth and biomass production (Lin et al, 2007; Peng et al, 2007; Sade et al, 2009; Khan et al, 2015; Li and Cai, 2015). Although some discrepancies exist, since overexpression phenotypes displaying sensitivity to abiotic stress have been observed (Wang et al, 2011), the current consensus is that TIPs are engaged in the regulation of important physiological processes that contributes to plant growth and adaptation to stress (reviewed in Afzal et al, 2016).…”

Section: Introductionmentioning

confidence: 94%

The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles

et al. 2016

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Plant aquaporins are water channels implicated in various physiological processes, including growth, development and adaptation to stress. In this study, the Tonoplast Intrinsic Protein (TIP) gene subfamily of Eucalyptus, an economically important woody species, was investigated and characterized. A genome-wide survey of the Eucalyptus grandis genome revealed the presence of eleven putative TIP genes (referred as EgTIP), which were individually assigned by phylogeny to each of the classical TIP1–5 groups. Homology modeling confirmed the presence of the two highly conserved NPA (Asn-Pro-Ala) motifs in the identified EgTIPs. Residue variations in the corresponding selectivity filters, that might reflect differences in EgTIP substrate specificity, were observed. All EgTIP genes, except EgTIP5.1, were transcribed and the majority of them showed organ/tissue-enriched expression. Inspection of the EgTIP promoters revealed the presence of common cis-regulatory elements implicated in abiotic stress and hormone responses pointing to an involvement of the identified genes in abiotic stress responses. In line with these observations, additional gene expression profiling demonstrated increased expression under polyethylene glycol-imposed osmotic stress. Overall, the results obtained suggest that these novel EgTIPs might be functionally implicated in eucalyptus adaptation to stress.

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Targeting Aquaporins for Conferring Salinity Tolerance in Crops

Kumar

Saddhe

2018

Salinity Responses and Tolerance in Plants, Volume 1

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A ginseng PgTIP1 gene whose protein biological activity related to Ser128 residue confers faster growth and enhanced salt stress tolerance in Arabidopsis

Cited by 12 publications

References 71 publications

Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants

Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants

The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles

Targeting Aquaporins for Conferring Salinity Tolerance in Crops

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