“…Sampling Samples were collected from 11 freshwater locations in southern British Columbia, Canada (49°49'4"N, 123° 7'46"W; 49°42'5"N, 123° 8'47"W; 49°37'34"N, 123°12'27"W; 49° 6'12"N, 122° 4'38"W; 49° 5'22"N, 122° 7'1"W; 49°18'10"N, 122°42'9"W; 49° 8'27"N; 123° 3'16"W; 49°13'21"N, 123°12'43"W; 49°13'13"N, 123°12'41"W; 49°14'52"N, 123°13'59"W; 49°15'58"N, 123°15'34"W. See Chapter 2). To concentrate pathogens, 20 liter water samples were prefiltered with a GF-A filter (Millipore, Bedford, MA, USA; nominal pore size 1.1 μm) over a 0.8-μm pore-size PES membrane (Sterlitech, Kent, WA, USA) [16]. Filtrates from all locations were pooled and concentrated using a 30kDa MW cut-off tangential flow ultrafiltration cartridge (Millipore, Bedford, MA, USA) [17].…”