A germination mutant of Bacillus subtilis deficient in response to glucose.

Irie, Ryozaburo; Okamoto, Takashi; Fujita, Yasuhito

doi:10.2323/jgam.28.345

Cited by 40 publications

(50 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transformation of B. subtilis was by the method of Anagnostopoulos & Spizizen (1961) and of E. ioli by the method of Mandel & Higa (1970). Ger phenotypes were scored on plate tests as described by Irie et al (1982).…”

Section: Methodsmentioning

confidence: 99%

The gerB region of the Bacillus subtilis 168 chromosome encodes a homologue of the gerA spore germination operon

Corfe

Sammons²,

Smith³

et al. 1994

Microbiology

View full text Add to dashboard Cite

Spores of gerB spore germination mutants of Bacillus subtilis 168 are defective in response to the germinative mixture of L-asparagine, glucose, fructose and potassium ions (AGFK), but are normal in the L-alanine (ALA) triggered germination response. A 3. clone of 15 kbp carrying the gerB region has been identified. Sequencing of the gerB region of the clone revealed a cluster of three ORFs encoding putative proteins of 53.3,41.3 and 42-4 kDa (GerBA, GerBB and GerBC, respectively). The first two of these proteins have substantial hydrophobic regions and the third i s a possible lipoprotein. A t least two, and probably all three products are required for normal germination in AGFK. The three proteins form a set of homologues of the products of the gerA operon, mutations in which cause a defect in the ALA germination pathway, but cause no defect in AGFK. The GerB proteins show 42%, 31 O/ O and 3 5 % identity a t the amino-acid level to the corresponding GerA proteins, and the homologues occur in the same order in both operons.

show abstract

Section: Methodsmentioning

confidence: 99%

The gerB region of the Bacillus subtilis 168 chromosome encodes a homologue of the gerA spore germination operon

Corfe

Sammons²,

Smith³

et al. 1994

Microbiology

View full text Add to dashboard Cite

show abstract

“…This protein (GerVB) seems to interact with GerUA and GerUC to form a functional receptor for glucose, L-proline, and KBr (Christie and Lowe, 2007). Interestingly, both GerUB and GerVB are orthologues of the GerKB subunit from B. subtilis and all three proteins are involved in glucose-mediated germination (Irie et al, 1982). This suggests that these three B-subunit proteins recognize glucose even though different organisms encode them.…”

Section: Bacillus Megaterium Ger Receptorsmentioning

confidence: 99%

The Ger Receptor Family from Sporulating Bacteria

Roß

Abel-Santos

2010

Current Issues in Molecular Biology

View full text Add to dashboard Cite

Bacterial spores are specialized cells that are exceptionally resistant to environmental stress. Spores convert back to actively growing cells, a process called germination, upon nutrient detection. The most common, initial step in the germination process is the recognition of small molecule germinants by germination (Ger) receptors. Ger receptors are inner-membrane heterocomplexes formed by three distinct protein products, the A-, B-, and C-subunits. In this review, we discuss and contrast published reports on representative Ger receptors from different Bacilli and Clostridia. We also present evidence for unrecognized germination pathways independent of Ger receptors. We further emphasize the function of L-alanine as a universal germinant. We also comment on biochemical aspects of germinant recognition and interaction between Ger receptor proteins. We propose that there are six general strategies used by Bacilli and Clostridia to integrate multiple germination signals. The use of different germinant recognition strategies results in germination response flexibility. Consequently, sporulating bacterial species that use the same biomolecules as germination signals can have different germination profiles. Finally, we discuss future directions for understanding the function of Ger receptors.

show abstract

“…Competent UOT0993 cells were transformed with the integrational plasmids (about 0.05ag/ml) and plated on a sporulation agar plate (6) containing CM (5 ,ag/ml). The transformants were allowed to sporulate and were examined for the Ger phenotype (AGFK) by the paper replica-tetrazolium method (5,6). For each plasmid, at least 50 transformant colonies were tested.…”

Section: Use Of Integrational Plasmids For Determining the End Of Thementioning

confidence: 99%

“…Two tetrazolium-white colonies were picked up and used as inocula for preparing clear spore suspensions. The Ger-phenotype was confirmed by the optical density method (6). No tetrazolium-white colony appeared in the control run in which the plasmid without deletion was used.…”

mentioning

confidence: 99%