A genomic approach to the understanding of Xylella fastidiosa pathogenicity

Lambais, Márcio Rodrigues; Goldman, Maria Helena S.; Camargo, L. E. A.; Goldman, Gustavo Henrique

doi:10.1016/s1369-5274(00)00121-1

Cited by 53 publications

(71 citation statements)

References 30 publications

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“…The four different but related B. quintana Vomp create the potential for adaptation to these different environments. The closely related XadA adhesin of the plant pathogen X. fastidiosa is hypothesized to be involved in binding to the insect vector foregut but not the plant host (40). One or more B. quintana Vomp may be expressed only in the arthropod vector (e.g., VompD, which is least homologous to the other Vomp, lacks collagen-binding motifs, and is most homologous with XadA).…”

Section: Discussionmentioning

confidence: 99%

A family of variably expressed outer-membrane proteins (Vomp) mediates adhesion and autoaggregation in Bartonella quintana

Zhang

Chomel

Schau

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

138

189

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Section: Discussionmentioning

confidence: 99%

A family of variably expressed outer-membrane proteins (Vomp) mediates adhesion and autoaggregation in Bartonella quintana

Zhang

Chomel

Schau

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

138

189

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“…More than 80% of the country's production comes from a single state, São Paulo, where the citrus industry and associated activities generate 3.4 million jobs and up to 1.5 billion U.S. dollars each year. In recent years, several diseases have affected this economically important crop and a great deal of attention has been focused on citrus variegated chlorosis (CVC), which was first reported in Brazil in 1987 (32) and has spread over at least 90% of the orchards in São Paulo (19). It seems that CVC is solely a disease of sweet-orange (Citrus sinensis L.) cultivars and is caused by Xylella fastidiosa (5,40), a xylem-limited gram-negative bacterial pathogen that is transmitted by xylem-feeding suctorial insects (sharpshooters) and is known to infect several other economically important plants, resulting in extensive economic losses (16).…”

mentioning

confidence: 99%

Diversity of Endophytic Bacterial Populations and Their Interaction with Xylella fastidiosa in Citrus Plants

Araújo

Marcon

Maccheroni

et al. 2002

Appl Environ Microbiol

495

350

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Citrus variegated chlorosis (CVC) is caused by Xylella fastidiosa, a phytopathogenic bacterium that can infect all Citrus sinensis cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. The endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (DGGE). Dominant isolates were characterized by fatty-acid methyl ester analysis as Bacillus pumilus, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium spp. (including Methylobacterium extorquens, M. fujisawaense, M. mesophilicum, M. radiotolerans, and M. zatmanii), Nocardia sp., Pantoea agglomerans, and Xanthomonas campestris. We observed a relationship between CVC symptoms and the frequency of isolation of species of Methylobacterium, the genus that we most frequently isolated from symptomatic plants. In contrast, we isolated C. flaccumfaciens significantly more frequently from asymptomatic plants than from those with symptoms of CVC while P. agglomerans was frequently isolated from tangerine (Citrus reticulata) and sweet-orange (C. sinensis) plants, irrespective of whether the plants were symptomatic or asymptomatic or showed symptoms of CVC. DGGE analysis of 16S rRNA gene fragments amplified from total plant DNA resulted in several bands that matched those from the bacterial isolates, indicating that DGGE profiles can be used to detect some endophytic bacteria of citrus plants. However, some bands had no match with any isolate, suggesting the occurrence of other, nonculturable or as yet uncultured, endophytic bacteria. A specific band with a high G؉C ratio was observed only in asymptomatic plants. The higher frequency of C. flaccumfaciens in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC.

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“…Type I pili, best studied in Escherichia coli, are associated with attachment of cells to substrata and development of biofilms (17,22). Adhesion to substrata by X. fastidiosa as well as by other bacterial species can also occur through the activity of afimbrial adhesins present on the cell surface (6,9,11,19,20); however, the pili of X. fastidiosa appear to often keep the bacterial cell surface from making intimate contact with the substratum, at least initially. Thus, contact with and adhesion of X. fastidiosa to substrata via pili are crucial for establishing spread and colonization.…”

mentioning

confidence: 99%

Assessing Adhesion Forces of Type I and Type IV Pili of Xylella fastidiosa Bacteria by Use of a Microfluidic Flow Chamber

Fuente

Montanes

Meng

et al. 2007

Appl Environ Microbiol

117

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Xylella fastidiosa, a bacterium responsible for Pierce's disease in grapevines, possesses both type I and type IV pili at the same cell pole. Type IV pili facilitate twitching motility, and type I pili are involved in biofilm development. The adhesiveness of the bacteria and the roles of the two pili types in attachment to a glass substratum were evaluated using a microfluidic flow chamber in conjunction with pilus-defective mutants. The average adhesion force necessary to detach wild-type X. fastidiosa cells was 147 ؎ 11 pN. Mutant cells possessing only type I pili required a force of 204 ؎ 22 pN for removal, whereas cells possessing only type IV pili required 119 ؎ 8 pN to dislodge these cells. The experimental results demonstrate that microfluidic flow chambers are useful and convenient tools for assessing the drag forces necessary for detaching bacterial cells and that with specific pilus mutants, the role of the pilus type can be further assessed.

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A genomic approach to the understanding of Xylella fastidiosa pathogenicity

Cited by 53 publications

References 30 publications

A family of variably expressed outer-membrane proteins (Vomp) mediates adhesion and autoaggregation in Bartonella quintana

A family of variably expressed outer-membrane proteins (Vomp) mediates adhesion and autoaggregation in Bartonella quintana

Diversity of Endophytic Bacterial Populations and Their Interaction with Xylella fastidiosa in Citrus Plants

Assessing Adhesion Forces of Type I and Type IV Pili of Xylella fastidiosa Bacteria by Use of a Microfluidic Flow Chamber

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