A genome‐wide set of 106 microsatellite markers for the blue tit (<i>Cyanistes caeruleus</i>)

Olano-Marin, Juanita; Dawson, Deborah A.; Girg, Alexander; Hansson, Bengt; Ljungqvist, Marcus; Kempenaers, Bart; Mueller, Jakob C.

doi:10.1111/j.1755-0998.2009.02777.x

Cited by 31 publications

(38 citation statements)

References 79 publications

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“…More precisely, loci that were designed based on or showed homology to zebra finch ( Taeniopygia guttata ). Expressed sequence tags (ESTs) were considered to be putatively functional, whereas markers designed using traditional cloning methods and with no homology to avian ESTs were considered to be neutral (for more details on loci development, see Olano‐Marin et al., 2010). It must be emphasized two important questions regarding this approach.…”

Section: Methodsmentioning

confidence: 99%

The strength of the association between heterozygosity and probability of interannual local recruitment increases with environmental harshness in blue tits

Ferrer

García‐Navas

Sanz

et al. 2016

Ecology and Evolution

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The extent of inbreeding depression and the magnitude of heterozygosity–fitness correlations (HFC) have been suggested to depend on the environmental context in which they are assayed, but little evidence is available for wild populations. We combine extensive molecular and capture–mark–recapture data from a blue tit (Cyanistes caeruleus) population to (1) analyze the relationship between heterozygosity and probability of interannual adult local recruitment and (2) test whether environmental stress imposed by physiologically suboptimal temperatures and rainfall influence the magnitude of HFC. To address these questions, we used two different arrays of microsatellite markers: 14 loci classified as neutral and 12 loci classified as putatively functional. We found significant relationships between heterozygosity and probability of interannual local recruitment that were most likely explained by variation in genomewide heterozygosity. The strength of the association between heterozygosity and probability of interannual local recruitment was positively associated with annual accumulated precipitation. Annual mean heterozygosity increased over time, which may have resulted from an overall positive selection on heterozygosity over the course of the study period. Finally, neutral and putatively functional loci showed similar trends, but the former had stronger effect sizes and seemed to better reflect genomewide heterozygosity. Overall, our results show that HFC can be context dependent, emphasizing the need to consider the role of environmental heterogeneity as a key factor when exploring the consequences of individual genetic diversity on fitness in natural populations.

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Section: Methodsmentioning

confidence: 99%

The strength of the association between heterozygosity and probability of interannual local recruitment increases with environmental harshness in blue tits

Ferrer

García‐Navas

Sanz

et al. 2016

Ecology and Evolution

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“…Primer sequences and annealing temperatures for the microsatellites are given in Table S1 (see also Olano-Marin et al [29] and Hansson et al [30]). The PCR-products were separated and visualized using an ABI 3730 capillary sequencer (Applied Biosystems), and the genotypes were scored with Genemapper 4.0 (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…In blue tits, an extensive number of nuclear microsatellites have been developed [29] and a first generation linkage map has been constructed [30]. This enabled us chose a set of markers with known linkage map position for our analyses.…”

Section: Introductionmentioning

confidence: 99%

Pronounced Fixation, Strong Population Differentiation and Complex Population History in the Canary Islands Blue Tit Subspecies Complex

et al. 2014

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Evolutionary molecular studies of island radiations may lead to insights in the role of vicariance, founder events, population size and drift in the processes of population differentiation. We evaluate the degree of population genetic differentiation and fixation of the Canary Islands blue tit subspecies complex using microsatellite markers and aim to get insights in the population history using coalescence based methods. The Canary Island populations were strongly genetically differentiated and had reduced diversity with pronounced fixation including many private alleles. In population structure models, the relationship between the central island populations (La Gomera, Tenerife and Gran Canaria) and El Hierro was difficult to disentangle whereas the two European populations showed consistent clustering, the two eastern islands (Fuerteventura and Lanzarote) and Morocco weak clustering, and La Palma a consistent unique lineage. Coalescence based models suggested that the European mainland forms an outgroup to the Afrocanarian population, a split between the western island group (La Palma and El Hierro) and the central island group, and recent splits between the three central islands, and between the two eastern islands and Morocco, respectively. It is clear that strong genetic drift and low level of concurrent gene flow among populations have shaped complex allelic patterns of fixation and skewed frequencies over the archipelago. However, understanding the population history remains challenging; in particular, the pattern of extreme divergence with low genetic diversity and yet unique genetic material in the Canary Island system requires an explanation. A potential scenario is population contractions of a historically large and genetically variable Afrocanarian population, with vicariance and drift following in the wake. The suggestion from sequence-based analyses of a Pleistocene extinction of a substantial part of North Africa and a Pleistocene/Holocene eastward re-colonisation of western North Africa from the Canaries remains possible.

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“…nlm.nih.gov/mapview/) genome assemblies, respectively (cf. Dawson et al, 2007;Backströ m et al, 2008a;Olano-Marin et al, 2009). This genotyping strategy enables comparisons of synteny, gene order and pattern of recombination in homologous chromosomal regions of Passeriformes and Galliformes, two phylogenetically highly diverged avian lineages (cf.…”

Section: Introductionmentioning

confidence: 99%

Avian genome evolution: insights from a linkage map of the blue tit (Cyanistes caeruleus)

et al. 2009

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We provide a first-generation linkage map of the blue tit (Cyanistes caeruleus), a passerine within the previously genetically uncharacterized family Paridae, which includes 91 orthologous loci with a single anchored position in the chicken (Gallus gallus) sequence assembly. The map consists of 18 linkage groups and covers 935 cM. There was highly conserved synteny between blue tit and chicken with the exception of a split on chromosome 1, potential splits on chromosome 4 and the translocation of two markers from chromosome 2 and 3, respectively, to chromosome 5. Gene order was very well conserved for the majority of chromosomes, an exception being chromosome 1 where multiple rearrangements were detected. Similar results were obtained in a comparison to the zebra finch (Taeniopygia guttata) genome assembly. The recombination rate in females was slightly higher than in males, implying a moderate degree of heterochiasmy in the blue tit. The map distance of the blue tit was B78% of that of the Wageningen chicken broiler population, and very similar to the Uppsala chicken mapping population, over homologous genome regions. Apart from providing insights into avian recombination and genome evolution, our blue tit linkage map forms a valuable genetic resource for ecological and evolutionary research in Paridae.

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A genome‐wide set of 106 microsatellite markers for the blue tit (Cyanistes caeruleus)

Cited by 31 publications

References 79 publications

The strength of the association between heterozygosity and probability of interannual local recruitment increases with environmental harshness in blue tits

The strength of the association between heterozygosity and probability of interannual local recruitment increases with environmental harshness in blue tits

Pronounced Fixation, Strong Population Differentiation and Complex Population History in the Canary Islands Blue Tit Subspecies Complex

Avian genome evolution: insights from a linkage map of the blue tit (Cyanistes caeruleus)

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