A Genome-Wide Screen in Macrophages Defines Host Genes Regulating the Uptake of Mycobacterium abscessus

Gilliland, Haleigh N.; Beckman, Olivia K.; Olive, Andrew J.

doi:10.1128/msphere.00663-22

Cited by 2 publications

(1 citation statement)

References 62 publications

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“…Additionally, Mycobacterium abscessus was found to cause increasing community- and hospital-acquired infections in humans ( 197 ). In 2023, a study transduced murine macrophages with a genome-wide knockout library to generally define and characterize important regulators in macrophage- M. abscessus interactions, such as sulfated glycosaminoglycans(sGAG) ( 198 ). They found that macrophage- M. abscessus interactions were mainly regulated by sGAG.…”

Section: Crispr Screening In Immune Cellsmentioning

confidence: 99%

High-throughput CRISPR technology: a novel horizon for solid organ transplantation

Li,

Chen,

et al. 2024

Front. Immunol.

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Organ transplantation is the gold standard therapy for end-stage organ failure. However, the shortage of available grafts and long-term graft dysfunction remain the primary barriers to organ transplantation. Exploring approaches to solve these issues is urgent, and CRISPR/Cas9-based transcriptome editing provides one potential solution. Furthermore, combining CRISPR/Cas9-based gene editing with an ex vivo organ perfusion system would enable pre-implantation transcriptome editing of grafts. How to determine effective intervention targets becomes a new problem. Fortunately, the advent of high-throughput CRISPR screening has dramatically accelerated the effective targets. This review summarizes the current advancements, utilization, and workflow of CRISPR screening in various immune and non-immune cells. It also discusses the ongoing applications of CRISPR/Cas-based gene editing in transplantation and the prospective applications of CRISPR screening in solid organ transplantation.

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Section: Crispr Screening In Immune Cellsmentioning

confidence: 99%