A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length

Liu, Ningning; Han, Tian Xu; Du, Li-Lin; Zhou, Jin-Qiu

doi:10.1038/cr.2010.107

Cited by 23 publications

(23 citation statements)

References 9 publications

(16 reference statements)

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“…Similar to our findings, Dbl2 colocalizes with Rad22 (Rad52) and Fml1 (Mph1), and the ability of Fml1 to form nuclear foci in response to replicative stress requires the presence of Dbl2 (Yu et al 2013). Further evidence for a conserved function of Mte1/Dbl2 comes from a genomewide screen that identified the dbl2Δ deletion mutant as having elongated telomeres (Liu et al 2010). The putative human ortholog of Mte1, ZGRF1, was identified in two large-scale studies for genes required for resistance to cross-linking agents (Smogorzewska et al 2010) and genes required for recombinational repair of an endonucleaseinduced DSB (Adamson et al 2012).…”

Section: Discussionsupporting

confidence: 62%

Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

et al. 2016

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Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.

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Section: Discussionsupporting

confidence: 62%

Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

et al. 2016

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“…If Tls1 regulates the function of these shelterin components, we expect loss of Tls1 to affect telomere length as well. Indeed, a screen for mutants that affect telomere length showed that tls1-1 cells significantly elongated telomeres ( 42 ). Our Southern blot analyses with a probe specific for telomeric repeats also showed that tls1Δ cells have much longer telomeres, to an extent similar to those of taz1Δ , rap1Δ and poz1Δ (Figure 3B ).…”

Section: Resultsmentioning

confidence: 99%

Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length

Wang

Tadeo

Hou

et al. 2014

Nucleic Acids Research

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Heterochromatin preferentially forms at repetitive DNA elements through RNAi-mediated targeting of histone-modifying enzymes. It was proposed that splicing factors interact with the RNAi machinery or regulate the splicing of repeat transcripts to directly participate in heterochromatin assembly. Here, by screening the fission yeast deletion library, we comprehensively identified factors required for telomeric heterochromatin assembly, including a novel gene tls1+. Purification of Tls1 and mass spectrometry analysis of its interacting proteins show that Tls1 associates with the spliceosome subunit Brr2. RNA sequencing analysis shows that the splicing of a subset of mRNAs are affected in tls1Δ cells, including mRNAs of shelterin components rap1+ and poz1+. Importantly, replacing rap1+ and poz1+ with their cDNAs significantly alleviated heterochromatin defects of tls1Δ cells, suggesting that the missplicing of shelterin components is the cause of such defects, and that splicing factors regulate telomeric heterochromatin through the proper splicing of heterochromatin factors. In addition to its role in telomeric heterochromatin assembly, Tls1-mediated splicing of shelterin mRNAs also regulates telomere length. Given that its human homologue C9ORF78 also associates with the spliceosome and is overexpressed in multiple cancer cell lines, our results suggest that C9ORF78 overexpression might alter the proper splicing of genes during cancer progression.

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“…Based on high throughput analysis, nup132⌬ cells share another phenotype of pli1⌬ cells, that is, highly elongated telomeres (40,43). Notably, like centromeres, telomeres are also clustered at the nuclear periphery and are subject to Pli1-dependent regulation (40,41).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the inactivation of STUbL, when combined with higher levels of SUMO (Fig. 1, A and B) (28,42,43), we analyzed Pli1 levels in nup132⌬ cells.…”

Section: Nuclear Pore Mutant Suppresses Phenotypes Of Stublmentioning

confidence: 99%

Pli1PIAS1 SUMO Ligase Protected by the Nuclear Pore-associated SUMO Protease Ulp1SENP1/2

Nie

Boddy

2015

Journal of Biological Chemistry

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Background: SUMO conjugation to the proteome critically controls cell growth, but mechanisms for regulating SUMO ligases are poorly defined. Results: Desumoylation of the major fission yeast SUMO ligase by a nuclear pore-associated protease protects it from proteolysis. Conclusion: Desumoylation of a SUMO ligase antagonizes autoinhibition of the SUMO pathway.Significance: These data demonstrate cooperation between STUbL and Cdc48(p97) in proteasome-mediated degradation of SUMO conjugates.

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A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length

Cited by 23 publications

References 9 publications

Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length

Pli1PIAS1 SUMO Ligase Protected by the Nuclear Pore-associated SUMO Protease Ulp1SENP1/2

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