2015
DOI: 10.1101/028308
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A genome-wide resource for the analysis of protein localisation inDrosophila

Abstract: 30The Drosophila genome contains >13,000 protein coding genes, the majority of 31 which remain poorly investigated. Important reasons include the lack of 32 antibodies or reporter constructs to visualise these proteins. Here we present a 33 genome-wide fosmid library of ≈10,000 GFP-tagged clones, comprising tagged 34 genes and most of their regulatory information. For 880 tagged proteins we have 35 created transgenic lines and for a total of 207 lines we have assessed protein 36 expression and localisation in … Show more

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Cited by 1 publication
(2 citation statements)
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“…Insertion sites of these traps are: between coding exons 1 and 2 for if, exons 1 and 2 for Ilk, exons 10 and 11 for rhea, exons 2 and 3 for Sdc, and at intronic position X 2,499,173 in domain II for trol (many variables exons exist in this gene). In vivo protein-fusion fluorescent reporters LanB1 fTRG.681 .sGFP and Ndg fTRG.638 .sGFP are not protein traps generated from the endogenous loci, but from transgenic fosmids containing an extra copy of the gene to which a C-terminal sGFP exon has been added [50]. LanB1.sGFP and Ndg.sGFP are functional on account of their ability to rescue LanB1 [50] and Ndg mutants (not shown).…”
Section: Contact For Reagent and Resource Sharingmentioning
confidence: 99%
See 1 more Smart Citation
“…Insertion sites of these traps are: between coding exons 1 and 2 for if, exons 1 and 2 for Ilk, exons 10 and 11 for rhea, exons 2 and 3 for Sdc, and at intronic position X 2,499,173 in domain II for trol (many variables exons exist in this gene). In vivo protein-fusion fluorescent reporters LanB1 fTRG.681 .sGFP and Ndg fTRG.638 .sGFP are not protein traps generated from the endogenous loci, but from transgenic fosmids containing an extra copy of the gene to which a C-terminal sGFP exon has been added [50]. LanB1.sGFP and Ndg.sGFP are functional on account of their ability to rescue LanB1 [50] and Ndg mutants (not shown).…”
Section: Contact For Reagent and Resource Sharingmentioning
confidence: 99%
“…In vivo protein-fusion fluorescent reporters LanB1 fTRG.681 .sGFP and Ndg fTRG.638 .sGFP are not protein traps generated from the endogenous loci, but from transgenic fosmids containing an extra copy of the gene to which a C-terminal sGFP exon has been added [50]. LanB1.sGFP and Ndg.sGFP are functional on account of their ability to rescue LanB1 [50] and Ndg mutants (not shown). mys.sGFP fTRG.932 (Integrin b) fosmid reporter was unable to rescue lethality of mys mutants (mys M2 and mys G4 ) and therefore was not used in this study.…”
Section: Contact For Reagent and Resource Sharingmentioning
confidence: 99%