A genome-wide landscape of mRNAs, miRNAs, lncRNAs, and circRNAs of skeletal muscles during dietary restriction in Mongolian horses

Bou, Tugeqin; Ding, Wenqi; Liu, Huiying; Gon, Wendian; Jia, Zijie; Dugarjaviin, Manglai; Bai, Ding

doi:10.1016/j.cbd.2023.101084

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…Finally, DAB (Diaminobenzidine) (Solarbio Science & Technology Co., Ltd., Beijing, China) was used for color development, followed by counterstaining of the nuclei, dehydration, and mounting. A bright-field microscope (Olympus Sales & Service Co., Ltd., Tokyo, Japan) was used for section observation [37,38].…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Single-Cell Transcriptome Sequencing Reveals Molecular Expression Differences and Marker Genes in Testes during the Sexual Maturation of Mongolian Horses

Liu,

Du,

et al. 2024

Animals

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This study aimed to investigate differences in testicular tissue morphology, gene expression, and marker genes between sexually immature (1-year-old) and sexually mature (10-year-old) Mongolian horses. The purposes of our research were to provide insights into the reproductive physiology of male Mongolian horses and to identify potential markers for sexual maturity. The methods we applied included the transcriptomic profiling of testicular cells using single-cell sequencing techniques. Our results revealed significant differences in tissue morphology and gene expression patterns between the two age groups. Specifically, 25 cell clusters and 10 cell types were identified, including spermatogonial and somatic cells. Differential gene expression analysis highlighted distinct patterns related to cellular infrastructure in sexually immature horses and spermatogenesis in sexually mature horses. Marker genes specific to each stage were also identified, including APOA1, AMH, TAC3, INHA, SPARC, and SOX9 for the sexually immature stage, and PRM1, PRM2, LOC100051500, PRSS37, HMGB4, and H1-9 for the sexually mature stage. These findings contribute to a deeper understanding of testicular development and spermatogenesis in Mongolian horses and have potential applications in equine reproductive biology and breeding programs. In conclusion, this study provides valuable insights into the molecular mechanisms underlying sexual maturity in Mongolian horses.

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Section: Immunohistochemical Stainingmentioning

confidence: 99%