A genome-wide association study of lateral root number for Asian cotton (Gossypium arboreum L.)

Abstract

Background The lateral root is one of the most important organs that constitute the root architecture system in plants. It can directly affect the contact area between plants and soil and plays an important role in plant structural support and nutrient absorption. Optimizing root architecture systems can greatly increase crop yields. This study was designed to identify the molecular markers and candidate genes associated with lateral root development in cotton and to evaluate correlations with yield and disease traits. Result The number of lateral roots for 14-day old seedlings was recorded for 215 Gossypium arboreum accessions. A correlation analysis showed that the number of lateral roots positively correlates with the sympodial branch node and seed index traits, but negatively correlates with lint percentage. A Genome-wide association study (GWAS) identified 18 significant SNPs with 19 candidate genes associated with the lateral root number. Expression analysis identified three genes (FLA12, WRKY29, and RBOHA) associated with lateral root development. Conclusion GWAS analysis identified key SNPs and candidate genes for lateral root number, and genes of FLA12, WRKY29, and RBOHA may play a pivotal role in lateral root development in Asian cotton.

Recent progression and future perspectives in cotton genomic breeding

“…An ortholog of the Arabidopsis GSTF9 gene also represents a potential target for both Fusarium and Verticillium wilt disease resistance (Du et al, 2018). Very recently, 83 loci (69 candidate genes), four loci (19 candidate genes), and 15 loci (40 candidate genes) were associated with seedling biomass‐related traits, lateral root number, and leaf traits, respectively (Hu et al, 2022a; 2022b; 2022c).…”