A genome-wide analysis of SWEET gene family in cotton and their expressions under different stresses

Zhao, Lingxia; Yao, Jinbo; Chen, Wei; Li, Yan; Lu, Youjun; Guo, Yan; Wang, Junyi; Li, Yuan; Liu, Ziyang; Zhang, Yongshan

doi:10.1186/s42397-018-0007-9

Cited by 22 publications

(19 citation statements)

References 61 publications

(98 reference statements)

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“…Transgenic plants overexpressing AtSWEET4 accumulated more glucose and fructose than wild-type plants, and displayed increases in plant size and freezing tolerance [73]. In the present study, almost half (12/25) of MtSWEET genes exhibited extensive responses to cold, salt, and drought stresses (Figure 6; Table S2), and similar results were also observed in banana [6], tea plant [39], and cotton [59,74]. It is worth noting that the transcript levels of seven MtSWEET genes ( MtSWEET1a , MtSWEET2b , MtSWEET3c , MtSWEET9b , MtSWEET13 , MtSWEET15c , and MtSWEET16 ) were significantly altered by at least two of the three treatments (Figure 6 and Figure 7; Table S2).…”

Section: Discussionsupporting

confidence: 81%

SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

Huang

et al. 2019

Plants

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SWEET (Sugars Will Eventually be Exported Transporter) proteins mediate the translocation of sugars across cell membranes and play crucial roles in plant growth and development as well as stress responses. In this study, a total of 25 SWEET genes were identified from the Medicago truncatula genome and were divided into four clades based on the phylogenetic analysis. The MtSWEET genes are distributed unevenly on the M. truncatula chromosomes, and eight and 12 MtSWEET genes are segmentally and tandemly duplicated, respectively. Most MtSWEET genes contain five introns and encode proteins with seven transmembrane helices (TMHs). Besides, nearly all MtSWEET proteins have relatively conserved membrane domains, and contain conserved active sites. Analysis of microarray data showed that some MtSWEET genes are specifically expressed in disparate developmental stages or tissues, such as flowers, developing seeds and nodules. RNA-seq and qRT-PCR expression analysis indicated that many MtSWEET genes are responsive to various abiotic stresses such as cold, drought, and salt treatments. Functional analysis of six selected MtSWEETs in yeast revealed that they possess diverse transport activities for sucrose, fructose, glucose, galactose, and mannose. These results provide new insights into the characteristics of the MtSWEET genes, which lay a solid foundation for further investigating their functional roles in the developmental processes and stress responses of M. truncatula.

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Section: Discussionsupporting

confidence: 81%

SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

Huang

et al. 2019

Plants

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“…Interestingly, there was significant variation among SWEET proteins in length, predicted isoelectric points, and classification into four clades. This indicates that different SWEET proteins may function in different microenvironments, consistent with previous studies of SWEET functions [43]. Due to their functional diversity, we examined expression patterns of JrSWEET genes in different tissues.…”

Section: Discussionsupporting

confidence: 81%

“…Sugar transporters play a key role in growth and development of many plant species [34,43,44]. SWEETs are critically important for sugar efflux, phloem loading, and nectar secretion [11,45].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Profiling and Phylogenetic Analysis of the SWEET Sugar Transporter Gene Family in Walnut and Their Lack of Responsiveness to Xanthomonas arboricola pv. juglandis Infection

Jiang

Balan

Assis

et al. 2020

IJMS

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Following photosynthesis, sucrose is translocated to sink organs, where it provides the primary source of carbon and energy to sustain plant growth and development. Sugar transporters from the SWEET (sugar will eventually be exported transporter) family are rate-limiting factors that mediate sucrose transport across concentration gradients, sustain yields, and participate in reproductive development, plant senescence, stress responses, as well as support plant-pathogen interaction, the focus of this study. We identified 25 SWEET genes in the walnut genome and distinguished each by its individual gene structure and pattern of expression in different walnut tissues. Their chromosomal locations, cis-acting motifs within their 5 regulatory elements, and phylogenetic relationship patterns provided the first comprehensive analysis of the SWEET gene family of sugar transporters in walnut. This family is divided into four clades, the analysis of which suggests duplication and expansion of the SWEET gene family in Juglans regia. In addition, tissue-specific gene expression signatures suggest diverse possible functions for JrSWEET genes. Although these are commonly used by pathogens to harness sugar products from their plant hosts, little was known about their role during Xanthomonas arboricola pv. juglandis (Xaj) infection. We monitored the expression profiles of the JrSWEET genes in different tissues of "Chandler" walnuts when challenged with pathogen Xaj417 and concluded that SWEET-mediated sugar translocation from the host is not a trigger for walnut blight disease development. This may be directly related to the absence of type III secretion system-dependent transcription activator-like effectors (TALEs) in Xaj417, which suggests different strategies are employed by this pathogen to promote susceptibility to this major aboveground disease of walnuts.

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“…Gene duplication events play an important role in the expansion of family genes and the conservation and variation of functions (Paterson et al, 2012;Li and Zhang, 2015). Analysis of gene duplication events in the ELF4s family in cotton revealed that the expansion of ELF4s were mainly due to segment duplication (Zhao et al, 2018;Wu et al, 2019). The ratio of nonsynonymous (Ka) substitution to a synonymous (Letunic et al, 2015) substitute ion was used to assess the selection pressure (Silva et al, 2020) activity assay; **P < 0.01, *P < 0.05.…”

Section: Discussion Expansion and Duplication Of Elf4 Gene Familymentioning

confidence: 99%

Genome-Wide Identification of the Early Flowering 4 (ELF4) Gene Family in Cotton and Silent GhELF4-1 and GhEFL3-6 Decreased Cotton Stress Resistance

Tian

Zhang

et al. 2021

Front. Genet.

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The early flowering 4 (ELF4) family members play multiple roles in the physiological development of plants. ELF4s participated in the plant biological clock’s regulation process, photoperiod, hypocotyl elongation, and flowering time. However, the function in the ELF4s gene is barely known. In this study, 11, 12, 21, and 22 ELF4 genes were identified from the genomes of Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum, and Gossypium barbadense, respectively. There ELF4s genes were classified into four subfamilies, and members from the same subfamily show relatively conservative gene structures. The results of gene chromosome location and gene duplication revealed that segmental duplication promotes gene expansion, and the Ka/Ks indicated that the ELF4 gene family has undergone purification selection during long-term evolution. Spatio-temporal expression patterns and qRT-PCR showed that GhELF4 genes were mainly related to flower, leaf, and fiber development. Cis-acting elements analysis and qRT-PCR showed that GhELF4 genes might be involved in the regulation of abscisic acid (ABA) or light pathways. Silencing of GhELF4-1 and GhEFL3-6 significantly affected the height of cotton seedlings and reduced the resistance of cotton. The identification and functional analysis of ELF4 genes in upland cotton provide more candidate genes for genetic modification.

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A genome-wide analysis of SWEET gene family in cotton and their expressions under different stresses

Cited by 22 publications

References 61 publications

SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

Genome-Wide Profiling and Phylogenetic Analysis of the SWEET Sugar Transporter Gene Family in Walnut and Their Lack of Responsiveness to Xanthomonas arboricola pv. juglandis Infection

Genome-Wide Identification of the Early Flowering 4 (ELF4) Gene Family in Cotton and Silent GhELF4-1 and GhEFL3-6 Decreased Cotton Stress Resistance

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