A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori

Tanaka, Hiromitsu; Ishibashi, Jun Ichiro; Fujita, Kosuke; Nakajima, Yuichi; Sagisaka, Aki; Tomimoto, Kazuya; Suzuki, Noriko; Yoshiyama, Mikio; Kaneko, Yasunari; Iwasaki, Takashi; Sunagawa, Tomoya; Yamaji, Katsuhiko; Asaoka, Ai; Mita, Kazuei; Yamakawa, Minoru

doi:10.1016/j.ibmb.2008.09.001

Cited by 321 publications

(364 citation statements)

References 192 publications

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“…5) We found that the intercellular factors involved in both pathways are well-conserved in the fruit fly and silkworm by silkworm genome analysis. 24) Moreover, Dorsal and Relish orthologs (BmRels and BmRelishs respectively) have been reported to participate in activation of AMP gene expression triggered by Grampositive and Gram-negative bacteria respectively. 27,28) These data suggest that B. mori possesses both Toll and IMD pathways, activated by Lys-type and DAP-type PGNs respectively.…”

Section: Discussionmentioning

confidence: 99%

“…[22][23][24] These AMPs are produced by injection of bacteria, crude lipopolysaccharide, and PGN. 23,25,26) The genes possess conserved B sites in the 5 0 -upstream region.…”

Section: Bombyx Morimentioning

confidence: 99%

“…27,28) Genome analysis has revealed that the single BmRel gene can produce two alternative splicing isoforms, BmRelA and BmRelB. 24) The two Rel proteins BmRelA and BmRelB have almost identical amino acids sequences (BmRelA possesses an additional 52 amino acid sequences at the N-terminus), but they differentially regulate AMP genes. 27) BmRelish also produces two alternative splicing products, BmRelish1 and BmRelish2.…”

Section: Bombyx Morimentioning

confidence: 99%

“…On the other hand, DAP-type PGN activates the IMD pathway and provokes target AMP genes, such as Diptericin, by Relish. A genome-wide analysis by KAIKOBLAST database (http://kaikoblast.dna.affrc.go.jp/) indicated that intercellular factors, including the Dorsal and Relish orthologs involved in these pathways, are well-conserved in B. mori, 24) suggesting that B. mori also possesses the Toll and IMD pathways, which is probably activated by Lys-and DAP-type PGN respectively. These data lead us to speculate that the Cecropin B1 and Attacin genes are regulated by both pathways, and that induction by the IMD pathway is stronger than by the Toll pathway.…”

Section: Ability Of Bmrels and Bmrelish To Activate The Promoters Of mentioning

confidence: 99%

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Correlation of Differential Expression of Silkworm Antimicrobial Peptide Genes with Different Amounts of Rel Family Proteins and Their Gene Transcriptional Activity

Tanaka

Sagisaka

Nakajima

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

“…[22][23][24] These AMPs are produced by injection of bacteria, crude lipopolysaccharide, and PGN. 23,25,26) The genes possess conserved B sites in the 5 0 -upstream region.…”

Section: Bombyx Morimentioning

confidence: 99%

Section: Bombyx Morimentioning

confidence: 99%

Section: Ability Of Bmrels and Bmrelish To Activate The Promoters Of mentioning

confidence: 99%

See 2 more Smart Citations

Correlation of Differential Expression of Silkworm Antimicrobial Peptide Genes with Different Amounts of Rel Family Proteins and Their Gene Transcriptional Activity

Tanaka

Sagisaka

Nakajima

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…In the silkworm B. mori, six major antimicrobial peptides, cecropin, lebocin, moricin, attacin, defensin and gloverin, are produced by injection of bacteria, LPS and peptidoglycan (Kaneko et al, 2007;Kato et al, 1993;Tanaka et al, 2008). Furthermore, attacin gene expression is up-regulated by the injection of Escherichia coli and continued at least for 48 hours mainly in the larvae fat bodies and hemocytes (Sugiyama et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Characterization of Attacin from the Swallowtail Butterfly, Papilio xuthus

Kim¹,

Hwang²,

Park³

et al. 2011

International Journal of Industrial Entomology

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Attacin is an insect antibacterial protein that plays an important role in immune response to injury and infection. In this report, we have isolated and characterized of cDNA encoding for the attacin from the immunized larvae of swallowtail butterfly, Papilio xuthus. A full length cDNA of P. xuthus attacin was obtained by employing annealing control primer (ACP)-based differential display PCR and 5' RACE. The complete P. xuthus attacin cDNA was comprised of 949 bp encoding a 250 amino acid precursor. It contains a putative 18 amino acid signal peptide sequence, a 42 amino acid propeptide sequence, and a 190 amino acid mature protein with a theoretical molecular mass of 19904.01 and a pI of 9.13. The putative mature protein of P. xuthus attacin showed 48-52% and 24-30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. Semiquantitive RT-PCR results revealed that the transcript of P. xuthus attacin gene was up-regulated at significant levels after injection with bacterial lipopolysaccharide (LPS). We sub-cloned cDNA fragment encoding mature P. xuthus attacin into the expression vector, highly expressed in E. coli BL21 cells, and its antibacterial activity was analyzed. Recombinant P. xuthus attacin evidenced considerably antibacterial activity against Gram-negative bacteria, E. coli ML 35 and Klebsiella pneumonia.

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Expression profiling of novel bacteria‐induced genes from the silkworm, Bombyx mori

Tanaka

Suzuki

Nakajima

et al. 2010

Arch Insect Biochem Physiol

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In this study, we have newly identified three bacteria-induced genes from the silkworm Bombyx mori by quantitative reverse transcriptase-polymerase chain reaction. One of these, eukaryotic initiation factor 4E-1 (eIF4E-1), is assumed to encode an eIF4E family, which plays a role in the initiation of translation as a mRNA cap-binding protein. The second gene is BmFOXG1, belonging to a family of forkhead transcription factors, FOXG1. The third gene is MBF2-related (MBF2-R) whose product has high homology to a co-activator protein MBF2 from B. mori. Although BmFOXG1 was up-regulated in the fat body in response to three kinds of bacteria, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, eIF4E-1 and MBF2-R were up-regulated by E. coli and B. subtilis, but not S. aureus, suggesting that bacteria possessing meso-diaminopimelic acid-containing peptidoglycan but not lysine-containing peptidoglycan activate eIF4E-1 and MBF2-R, probably through a conserved immune deficiency pathway. We further profiled the expression of three genes in different tissues and a silkworm cell line, NIAS-Bm-aff3, in response to bacteria, and at different times after bacterial challenge in the fat body.

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A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori

Cited by 321 publications

References 192 publications

Correlation of Differential Expression of Silkworm Antimicrobial Peptide Genes with Different Amounts of Rel Family Proteins and Their Gene Transcriptional Activity

Correlation of Differential Expression of Silkworm Antimicrobial Peptide Genes with Different Amounts of Rel Family Proteins and Their Gene Transcriptional Activity

Molecular Cloning and Characterization of Attacin from the Swallowtail Butterfly, Papilio xuthus

Expression profiling of novel bacteria‐induced genes from the silkworm, Bombyx mori

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