A genipin crosslinked silk fibroin monolith by compression molding with recovering mechanical properties in physiological conditions

Bucciarelli, Alessio; Janigro, Valentino; Yang, Yuejiao; Fredi, Giulia; Pegoretti, Alessandro; Motta, Antonella; Maniglio, Devid

doi:10.1016/j.xcrp.2021.100605

Cited by 18 publications

(28 citation statements)

References 59 publications

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“…Other silk-related materials were developed with the specific purpose of structural applications [ 11 , 25 ]. The rheological test performed gave results of G′ and G″ higher than those reported in literature [ 39 ]; this is easily understandable, considering that, even though the starting material was the same, the crosslinking degree after UV-exposure depends on a series of other factors, which vary from experiment to experiment (adsorbed energy, wavelength of the UV lamp, concentration of Sil-MA, and amount of LAP).…”

Section: Discussionmentioning

confidence: 99%

“…Chemical crosslinking can be obtained using two approaches. The first consists of promoting the formation of dityrosine and trityrosine crosslinking by the use of different agents with or without an external stimulus (ruthenium and ammonium persulfate and UV irradiation [ 22 ], riboflavin and UVA irradiation [ 23 ], hydrogen peroxide and horseradish peroxidase [ 24 ], genipin in presence of humidity [ 25 , 26 ]). The alternative approach is based on the chemical modification of the main SF chain to add reactive moieties that can be activated by external stimuli, like UV light, able to trigger a condensation reaction.…”

Section: Introductionmentioning

confidence: 99%

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Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli

Petretta

Grigolo

et al. 2022

Gels

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Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light, which makes this material applicable in additive manufacturing techniques and allows the prototyping and development of patient-specific 2D or 3D constructs. In this study, we produced a thin grid structure based on crosslinked Sil-MA that can be withdrawn and ejected and that can recover its shape after rehydration. A complete chemical and physical characterization of Sil-MA was first conducted. Additionally, we tested Sil-MA biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility of using it in future trials. Sil-MA was also tested to verify its ability to support osteogenesis. Overall, Sil-MA was shown to be biocompatible and osteoconductive. Finally, two different additive manufacturing technologies, a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique, were used to develop a Sil-MA grid construct. A proof-of-concept of its shape-memory property was provided. Together, our data support the hypothesis that Sil-MA grid constructs can be injectable and applicable in bone regeneration applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli

Petretta

Grigolo

et al. 2022

Gels

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“…This resin was proved to reach compression modulus in the order of a gigapascal, however when tested after a prolonged period in a pseudo physiological condition their mechanical resistance dramatically dropped. A chemical crosslinking was obtained by the addition of Genipin to fibroin prior to the compression [27]. The crosslinking reaction triggered by the increase in temperature and the presence of water allowed to obtain a chemically crosslinked resin with a compression modulus high enough to be used in structural applications even in wet environments as the biological one [27].…”

Section: Of 16mentioning

confidence: 99%

Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition Of Citric Acid

Bucciarelli¹,

Vighi²,

Bossi³

et al. 2023

Preprint

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Thermoplastic molded regenerated silk was proposed as structural material in tissue engineering applications, mainly for application in bone. The protocol allows to obtain a compact non-porous material with a compression modulus in the order of a Giga Pascal starting from silk fibroin by compressing a silk fibroin lyophilized sponge of powder in mold at temperature higher than the glass transition temperature (Tg). The main purpose of the produced resin was the osteofixation and other structural applications in which the lack of porosity was not an issue. In this work, we introduced the use of citric acid in the thermoplastic molding protocol of SF to obtain a porosity inside the structural material. In addition to the previously developed protocols the addition of citric acid allowed us to obtain a structural material. The CA powder during the compression acted as a template for the pore formation. In addition, the CA was able to effectively crosslink the SF chain improving the mechanical strength. This effect was proved both evaluating the compression modulus and by studying the spectra obtained by Fourier infrared spectroscopy (FTIR). This protocol may be applied in the near future to the production of a structural bone scaffold.

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“…Chemical crosslinking can be obtained by two approaches. The first consists in promoting the formation of dityrosine and trityrosine crosslinking by the use of different agents with or without an external stimuli (ruthenium and ammonium persulphate and UV irradiation [24], riboflavin and UVA irradiation [25], hydrogen peroxide and horseradish peroxidase [26], Genipin in presence of humidity [27,28]). The latter is a modification of the protein to allow a crosslinking reaction triggered by an on/off UV exposure (photocrosslinking).…”

Section: Of 21mentioning

confidence: 99%

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli¹

2022

Preprint

View full text Add to dashboard Cite

Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light. This allows the structuring of this material throught additive manufacturing techniques and then to easily prototype patient specific construct. In this study we used Sil-MA to produce single layer crosslinked structures that can be withdrawal and ejected recovering their shape after rehydration. A complete chemical and physical characterization of the material has been conducted. Additionally, we tested the material biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility to use it in future trials. The material was also tested to verify its ability to support the osteogenesis. Two different additive manufacturing techniques have been tested (a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique) to develop Sil-MA grid. Finally, we provide a proof-of-concept that the printed Sil-MA structures are injectable.

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A genipin crosslinked silk fibroin monolith by compression molding with recovering mechanical properties in physiological conditions

Cited by 18 publications

References 59 publications

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition Of Citric Acid

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

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