A Genetical Factor Enhancing Plant Regeneration Lined with the V-v Locus in Hordeum vulgare L.

Komatsuda, Takao; Lee, Wenbin; Satô, Hiroshi; Annaka, T.; Enomoto, Sueo; Kang, Min-Jung; Oka, Seibi

doi:10.1270/jsbbs1951.41.661

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…The QTL for green plant regeneration linked to abg019‐abc162 on chromosome 2H (this study) is probably analogous to the previously identified QTLs Qsr1 and Shd1 Komatsuda and colleagues (Komatsuda et al, 1991, 1993, 1995) studied shoot regeneration in the progeny of a cross between a two‐rowed and a six‐rowed cultivar, and described the locus Shd1 and its close linkage to the V‐v locus that controls the fertility of lateral florets (2‐ versus 6‐rowed heads); this region is within 10 cM of the QTL linked to the interval abg019‐abc162 identified in this study. Qsr1 (Mano et al, 1996) was placed between the markers abg316c and abc167b; abc162 (this study) is located within this interval.…”

Section: Resultssupporting

confidence: 83%

“…Komatsuda et al (1991)(1993, 1995) and Mano et al (1996) identified QTLs for green plant regeneration from barley callus. Mano et al studied the same population (Steptoe/Morex‐derived DH lines) as was studied for this report, whereas Komatsuda's group studied Japanese cultivars that are only distantly related to the U.S.‐developed Steptoe and Morex cultivars.…”

Section: Resultsmentioning

confidence: 99%

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Genetic Markers Associated with Green and Albino Plant Regeneration from Embryogenic Barley Callus

Bregitzer

Campbell

2001

Crop Science

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Genetic control of plant regeneration from cultured plant tissues has been documented for a number of species. The characterization and manipulation of loci that influence morphogenic responses may be useful for the development of highly regenerable germplasm or for physiological investigations. Quantitative trait loci (QTLs) for morphogenesis from barley cell cultures were identified on the basis of associations of mapped markers with the regeneration responses of 77 doubled haploid (DH) lines derived from the cross Steptoe/Morex. Two models were developed, one describing green plant regeneration, and one describing albino plant regeneration, measured as the numbers of green and albino plants regenerated per gram fresh weight of embryogenic callus. Approximately 62 and 12% of the observed variability for green and albino plant regeneration, respectively, was explained by the models. An independent data set was developed that consisted of the regeneration responses of 25 additional DH lines which were chosen randomly from the same segregating population. The models were tested for their ability to predict the responses of these independent DH lines. This study identified new QTLs for plant regeneration (one for green plants and at least one for albino plants), and confirmed previously reported associations of three QTLs with green plant regeneration.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Genetic Markers Associated with Green and Albino Plant Regeneration from Embryogenic Barley Callus

Bregitzer

Campbell

2001

Crop Science

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“…Immature embryos are currently used as reliable and efficient target tissues for genetic transformation (Cho et al 2002, Scholz et al 2001, Zhang et al 2001. In barley, the light condition for callus induction in immature embryo cultures differed with the reports, even when calli were cultured under light during shoot regeneration (Cho et al 1998, Komatsuda et al 1991, Mano et al 1996, Rikiishi et al 2003b, Wan and Lemaux 1994. Cho et al (1998) suggested that highly regenerative structures of barley can be cultured under dim light for more than a year without marked loss of regeneration ability.…”

Section: Introductionmentioning

confidence: 99%

Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos

et al. 2008

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Callus growth and shoot regeneration were examined in cultures of immature barley embryos incubated under various combinations of a week under a 16-h photoperiod and a week under continuous darkness. Incubation in darkness for four weeks, during which calli were formed, enhanced shoot regeneration in 'K-3' and 'Kanto Nijo-5', but inhibited it in 'Lenins'. 'K-3' and 'Kanto Nijo-5', incubated in darkness during the first two weeks of callus induction, followed by two weeks of a 16-h photoperiod (D2L2), showed a higher percentage of shoot regeneration than those incubated for two weeks with a 16-h photoperiod followed by two weeks of darkness (L2D2). Nevertheless, when the light conditions, D2L2 and L2D2, were combined into the same periods of a 16-h photoperiod and continuous darkness, except for the order, light conditions affected shoot regeneration differently. The early stage of callus induction seems to be sensitive to light. The expression ratios of the auxin-responsive gene (AUX/IAA) to the cytokinin-responsive gene (WPK4) were increased in continuous darkness in 'K-3' and 'Kanto Nijo-5'. The effects of light qualities (white, red, farred, and blue) on callus growth and shoot regeneration were also examined. Blue light inhibited shoot regeneration, as did white light, in 'K-3' and 'Kanto Nijo-5', and far-red light in 'Kanto Nijo-5'. Light probably controls shoot regeneration from calli by modifying cytokinin levels and/or response; blue light signals act in photo-inhibition of shoot regeneration in immature barley embryo culture.

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Genetic mapping of a quantitative trait locus (QTL) that enhances the shoot differentiation rate in Hordeum vulgare L.

Komatsuda

Annaka²,

Oka

1993

Theoret. Appl. Genetics

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A quantitative trait locus (QTL) controlling shoot differentiation from immature embryo callus was identified by linkage analysis with morphological and isozyme markers in barley, Hordeum vulgare L. Immature embryos were isolated from cvs 'Azumamugi' (difficult to differentiate), 'Kanto Nakate Gold' (easy to differentiate), their hybrids (F1) and a backcross population derived from a cross 'Azumamugi' x F1. The embryos were cultured in vitro for callus initiation and subsequent shoot differentiation. The shoot differentiation rate was closely associated with ear type (v locus), isocitrate dehydrogenase isozyme (Idh-2), and esterase isozyme (Est-11). These markers were found to reside in a chromosome segment of approximately 30cM on chromosome 2. Recombination frequency was 9.9% between v and a proposed QTL named "Shd1" (shoot differentiation), 11.5% between Idh-2 and Shd1, and 21.3% between Est-11 and Shd1. All data showed the Idh-2, v, Shdl and Est-11 loci to be arranged in this order from proximal to distal on the long arm of chromosome 2.

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A Genetical Factor Enhancing Plant Regeneration Lined with the V-v Locus in Hordeum vulgare L.

Cited by 6 publications

References 5 publications

Genetic Markers Associated with Green and Albino Plant Regeneration from Embryogenic Barley Callus

Genetic Markers Associated with Green and Albino Plant Regeneration from Embryogenic Barley Callus

Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos

Genetic mapping of a quantitative trait locus (QTL) that enhances the shoot differentiation rate in Hordeum vulgare L.

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