A genetic system to detect mitotic recombination between repeated chromosomal sequences in Drosophila Schneider line 2 cells

Bärtsch, Stephan; Würgler, Friedrich E.; Sengstag, Christian

doi:10.1016/s1383-5718(97)00138-1

Cited by 6 publications

(5 citation statements)

References 58 publications

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“…Of those, some down-regulated proteins are prominent members of a family of microtubule-associated proteins linked to abnormal brain development and cortical lamination defects in humans (i.e. Tubb2b, Dcx) 34,35 . However, as Cul3 targets proteins for degradation, we were most interested in the identified up-regulated proteins, as their up-regulation likely represents a direct consequence of Cul3 loss.…”

Section: Resultsmentioning

confidence: 99%

Cul3regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development

Morandell

Schwarz

Basilico

et al. 2020

Preprint

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11De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 (CUL3) lead to 12 autism spectrum disorder (ASD). Here, we used Cul3 mouse models to evaluate the 13 consequences of Cul3 mutations in vivo. Our results show that Cul3 haploinsufficient mice 14 exhibit deficits in motor coordination as well as ASD-relevant social and cognitive impairments. 15Cul3 mutant brain displays cortical lamination abnormalities due to defective neuronal migration 16 and reduced numbers of excitatory and inhibitory neurons. In line with the observed abnormal 17 columnar organization, Cul3 haploinsufficiency is associated with decreased spontaneous 18 excitatory and inhibitory activity in the cortex. At the molecular level, employing a quantitative 19 proteomic approach, we show that Cul3 regulates cytoskeletal and adhesion protein abundance 20 in mouse embryos. Abnormal regulation of cytoskeletal proteins in Cul3 mutant neuronal cells 21 results in atypical organization of the actin mesh at the cell leading edge, likely causing the 22 observed migration deficits. In contrast to these important functions early in development, Cul3 23 deficiency appears less relevant at adult stages. In fact, induction of Cul3 haploinsufficiency in 24 adult mice does not result in the behavioral defects observed in constitutive Cul3 25

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Section: Resultsmentioning

confidence: 99%

Cul3regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development

Morandell

Schwarz

Basilico

et al. 2020

Preprint

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“…High-level expression in D. melanogaster and Aedes spp. cell lines is usually associated with the integration of multi-copy head-to-tail transgene arrays [ 17 , 18 , 39 , 40 ]. We also observed such arrays in our monoclonal line producing high levels of EGFP, increasing the fluorescence signal by tenfold compared to the polyclonal line.…”

Section: Discussionmentioning

confidence: 99%

“… Method Source of growth factors Cell lines Ref. Limiting dilution Conditioned medium Drosophila melanogaster S2, Spodoptera frugiperda Sf 9 [ 18 ] (a) [ 19 ] (a) Feeder cells - mitomycin C D. melanogaster S2 [ 20 ] (a) Feeder cells - irradiated D. melanogaster S2 [ 21 , 17 , 22 ] (a,b) Feeder cells - spatially separated D. melanogaster imaginal disc [ 23 ] (a) Feeder cells - untreated, living D. melanogaster S2 [ 8 , 9 , 10 , 24 , 25 , 26 ] (a,b) Soft agar Conditioned medium D. melanogaster S2 [ 18 , 27 ] (a) Feeder cells - irradiated D. melanogaster S2 [ 17 , 28 ] (a) …”

Section: Introductionmentioning

confidence: 99%

Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression

Zitzmann

Schreiber

Eichmann

et al. 2018

Biotechnology Reports

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“…Recombination in mitotic cells is reported for several species, ranging from placental mammals (Cornforth & Eberle, 2001;Svetlova et al, 2001) to insects (Stern, 1936;Bartsch et al, 1997) and yeast (Huang & Keil, 1995). For example, in the yeast Saccharomyces cerevisiae, the recombination hotspot HOT1 initiates mitotic recombination when inserted into novel locations throughout the genome (Huang & Keil, 1995).…”

Section: Discussionmentioning

confidence: 99%

Distribution of heterochromatin and rDNA on the holocentric chromosomes of the aphids Dysaphis plantaginea and Melanaphis pyraria (Hemiptera: Aphididae)

Criniti¹,

Simonazzi²,

Cassanelli³

et al. 2009

Eur. J. Entomol.

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Abstract. The structure of the holocentric chromosomes of the rosy apple aphid, Dysaphis plantaginea (2n = 12), and pear-grass aphid, Melanaphis pyraria (2n = 8), was studied using C-banding, NOR, Giemsa and fluorochrome staining, and fluorescent in situ hybridization (FISH). Contrary to the equilocal distribution of heterochromatin typical of monocentric chromosomes, in both species C-banding evidenced a tendency of highly repetitive DNAs to be restricted to the X chromosomes. Silver staining and FISH, using a 28S rDNA probe, located rDNA genes on one telomere of each X chromosome, the only brightly fluorescent C-positive sites revealed by CMA3 staining, whereas all other heterochromatic C-bands were DAPI positive. Both species showed a noticeable amount of rDNA heteromorphism. Mitotic recombination is proposed as a possible mechanism responsible for the variation in size of rDNA.

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A genetic system to detect mitotic recombination between repeated chromosomal sequences in Drosophila Schneider line 2 cells

Cited by 6 publications

References 58 publications

Cul3regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development

Cul3regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development

Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression

Distribution of heterochromatin and rDNA on the holocentric chromosomes of the aphids Dysaphis plantaginea and Melanaphis pyraria (Hemiptera: Aphididae)

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