A genetic map of common bean to localize specific resistance genes against anthracnose

Abstract

A bean genetic map was developed to locate resistance genes against anthracnose and genes involved in plant defense mechanisms. One hundred and fifty-seven markers (51 restriction fragment length polymorphism, 100 random amplified polymorphic DNA, 2 sequence characterized amplified regions, and 4 morphological markers) were used to construct a genetic map covering 567.5 cM of the bean genome. Morphological markers consisted in two resistance genes towards anthracnose (Are and RVI), a dominant gene for nuclear male sterility (Ms8) and a pod-shape character (SGou). This map was established by using a backcross population (BC1) of 128 individuals, derived from a cross between two European bean genotypes: Ms8EO2 and Corel. Nine percent of the markers showed segregation distortions and mapped to three regions. Clusters of 2-10 markers were observed in every linkage group. The possible origin of these clusters is discussed. Nineteen markers shared with a previously published bean linkage map allowed us to establish a preliminary correspondence between the two maps. Finally, seven genes involved in plant defense mechanisms were located on this map.

Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea

“…Therefore, with a few exceptions, the different maps included few shared markers. The exceptions included the use of shared RFLP markers between the map developed at the University of California, Davis (Nodari et al, 1993a) and the map developed at the University of Paris XI (Adam-Blondon et al, 1994), along with a few isozyme and seed protein markers also shared with the map developed at the University of Florida Gepts et al (1993), Nodari et al (1993a) and Freyre et al (1998) BAT93  Jalo EEP558 (RI) a BJ Midas  G12873 (RI) MG Domestication syndrome: Ppd, fin, St, y, P; phenology, number of nodes and pods, seed weight; dormancy Koinange et al (1996) XR235-1-1  DIACOL Calima (BC) XD Resistance to: X. axonopodis Vallejos et al (1992) and Yu et al (1998) ( Vallejos et al, 1992). The total number of the shared markers, however, remained quite small.…”

“…For each of the LGs, a minimum of two (or more) markers from the different maps were mapped in the BJ population. The maps involved included the Davis map (Nodari et al, 1993a;Freyre et al, 1998), the Paris map (Adam-Blondon et al, 1994), the Florida map (Vallejos et al, 1992), and the Nebraska-Wisconsin maps (Jung et al, 1996a(Jung et al, , 1997. The former three maps are based on RFLP markers, wholly or in their majority.…”

“…Fr is a male fertility restorer gene (He et al, 1995). SGou and Ms-8 are genes controlling pod cross-section and male sterility (Adam-Blondon et al, 1994). To the right (boxed symbols), are QTL mapped in different populations (Table 1)-ANT: anthracnose; APP: appearance of canned kidney beans; ASB: ashy stem blight; BGMV: bean golden mosaic virus; CBB: common bacterial blight resistance; DF and DM: number of days to flowering and to maturity; DO: seed dormancy; FRR: Fusarium root rot; FW: Fusarium wilt; HB: halo blight; HI: harvest index; HT: height; L5: length of the 5th internode; LDG: lodging; NM: number of nodes on the main stem; NN: Rhizobium nodulation; NP: number of pods; PD: photoperiodinduced delay in flowering; PL: pod length; SW: seed weight; WB: web blight; WM: white mold.…”

Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps

“…This map comprises 51 RFLP marker loci, 11 of which were detected with PstI clones developed at the University of California, Davis (see above). It also includes 100 RAPDs two sequence-characterized amplified regions (SCARs), and four morphological markers (Adam-Blondon et al 1994b). …”

“…In recent years, several restriction fragment length polymorphism (RFLP)-and random amplified polymorphic DNA (RAPD)-based linkage maps have been published for common bean. RFLP-containing maps include those of the University of California, Davis (Nodari et al 1993a;Gepts et al 1993), the University of Florida Boutin et al 1995;Yu et al 1998), and the University of Paris-Orsay (Adam-Blondon et al 1994b) maps (henceforth, Davis, Florida, and Paris maps). The first two are based primarily on RFLPs revealed by probes from different PstI and EcoRI-BamHI genomic libraries, while the latter is based mainly on RAPD markers but also contains RFLP markers.…”