A Genetic Map Based on RAPD, RFLP, Isozyme, Morphological Markers and QTL Analysis for Clubroot Resistance in Brassica oleracea.

Moriguchi, Katsumi; Kimizuka-Takagi, Chiaki; Ishii, Kenji; Nomura, Kazunari

doi:10.1270/jsbbs.49.257

Cited by 58 publications

(39 citation statements)

References 36 publications

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“…Similarly, a QTL found by Rocherieux et al (2004) at the end of a linkage group explained 20 to 87% of the variation depending on the pathogen isolates used. In addition, Moriguchi et al (1999) found an effective QTL at the end of a linkage group. These studies suggest that a CR gene at the end of a linkage group has a major effect in B. oleracea.…”

Section: B Oleraceamentioning

confidence: 97%

“…Mapping studies using molecular markers also revealed polygenic nature of the CR trait in B. oleracea (Landry et al 1992, Figdore et al 1993, Grandclément and Thomas 1996, Voorrips et al 1997, Moriguchi et al 1999, Rocherieux et al 2004, Nomura et al 2005. However, these studies showed the presence of a CR gene having large effect in this species.…”

Section: B Oleraceamentioning

confidence: 98%

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Genetic Analysis of Clubroot Resistance in Brassica Crops

Hirai

2006

Breed. Sci.

108

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Clubroot disease is caused by an obligate parasite, Plasmodiophora brassicae, and is one of the most serious diseases of Brassica crops worldwide. The pathogen is a eukaryote, and is a member of Plasmodiophorales. Recent phylogenic studies did not reveal its close relationship to fungi, but suggest a classification in Protozoa or Protoctista. The infection is thought to occur through two phases. The pathogen induces massive clubs on root, and prevents host growth. A number of clubroot resistance (CR) Chinese cabbage (Brassica rapa) cultivars have been bred using European turnips as sources of CR genes. Field populations of the pathogen have a wide variation of virulence, which causes the infection of CR Chinese cabbage cultivars. The CR gene of B. rapa is extensively studied with the aid of molecular markers. At least four independent loci are identified and mapped. They are all derived from European turnips. Crr1, and Crr2 are derived from Siloga and mapped in linkage groups R8 and R6, respectively. Crr3 from Milan White, and CRb from Gelria R are both mapped in R3. The molecular markers linked to Crr1, Crr2 and CRb showed homology to the central part of the long arm of chromosome 4 of Arabidopsis thaliana. These findings suggest that these CR genes are derived from the same region of the ancestral genome. In contrast, Crr3 is thought to have different origin, since its linkage markers showed homology to chromosome 3 of A. thaliana. Although another CR gene CRa is reported, the relationship between above mentioned four CR loci and CRa is not yet clarified. Genetic studies on CR genes in B. oleracea revealed a polygenic nature of the trait in this species and also suggest the presence of one CR gene having large effect. However, correspondence among the CR genes reported in these studies is not clarified because of the lack of information on base sequence of the linkage markers in this species. Strategies for breeding of more resistant Chinese cabbage cultivars to overcome the variation on virulence of field populations of the pathogen are presented.

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Section: B Oleraceamentioning

confidence: 97%

Section: B Oleraceamentioning

confidence: 98%

Genetic Analysis of Clubroot Resistance in Brassica Crops

Hirai

2006

Breed. Sci.

108

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“…Moreover, synthetic amphidiploid Raphanobrassica, which was developed by the hybridization of Raphanus and Brassica, also have high levels of clubroot resistance (McNaughton 1973, Yoshikawa 1981. However, the CR gene(s) of radish has not been investigated in comparison with that of Brassica crops such as B. rapa (2n = 20, AA), B. oleracea (2n = 18, CC), and B. napus (Kuginuki et al 1997, Moriguchi et al 1999, Manzanares-Dauleux et al 2000, Suwabe et al 2003, 2006, Hirai et al 2004, Nomura et al 2005. Although QTL analysis was conducted previously by Kamei et al (2007), the clubroot resistance of radish has not been investigated at the chromosomal level.…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line

et al. 2009

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Clubroot resistance (CR) against the Ano-01 isolate of the clubroot pathogen Plasmodiophora brassicae for individual radish chromosomes was evaluated using 9 types (a-i) of Brassica napus-Raphanus sativus monosomic addition lines (MALs) produced from the progenies of the hybridization of B. napus (2n = 38, AACC) and Raphanobrassica 'Rb-63' (2n = 36, RRCC). Among the 9 types of MAL, the c-type showed high resistance, although other types and reverted plants with somatic chromosomes like B. napus (2n = 38) were susceptible. This result suggested that a major CR gene against the Ano-01 isolate may be located on the radish c-chromosome. However, it was also assumed that there might be other CR gene(s) in radish because many c-type MAL plants showed slight disease symptoms.

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“…(Crute , 1980;Hansen, 1989;Voorrips Visser, 1993), (Laurence Thomas, 1993). (Figdore , 1993;Grandclement Thomas, 1996;Landry , 1992;Moriguchi , 1999;Nomura , 2005;Rocherieux , 2004;Voorrips , 1997). (Voorrips, 1995).…”

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confidence: 99%

Evaluation of Cabbage- and Broccoli-genetic Resources for Resistance to Clubroot and Fusarium Wilt

Lee¹,

Jo²,

Jang³

et al. 2014

Research in Plant Disease

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Clubroot and Fusarium wilt of cole crops (Brassica oleracea L.) are destructive diseases which for many years has brought a decline in quality and large losses in yields all over the world. The breeding of resistant cultivars is an effective approach to reduce the use of chemical fungicides and minimize crop losses. This study was conducted to evaluate the resistance of 60 cabbage (B. oleracea var. capitata) and 6 broccoli (B. oleracea var. italica) lines provided by The RDA-Genebank Information Center to clubroot and Fusarium wilt. To investigate resistance to clubroot, seedlings of the genetic resources were inoculated with Plasmodiophora brassicae by drenching the roots with a mixed spore suspension (1 : 1) of two isolates. Of the tested genetic resources, four cabbage lines were moderately resistant and 'K166220' represented the highest resistance to P. brassicae. The others were susceptible to clubroot. On the other hand, to select resistant plants to Fusarium wilt, the genetic resources were inoculated with Fusarium oxysporum f. sp. conglutinans by dipping the roots in spore suspension of the fungus. Among them, 17 cabbage and 5 broccoli lines were resistant, 16 cabbage lines were moderately resistant, and the others were susceptible to Fusarium wilt. Especially, three cabbage ('IT227115', 'K161791', 'K173350') and two broccoli ('IT227100', 'IT227099') lines were highly resistant to the fungus. We suggest that the resistant genetic resources can be used as a basic material for resistant B. oleracea breeding system against clubroot and Fusarium wilt.

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A Genetic Map Based on RAPD, RFLP, Isozyme, Morphological Markers and QTL Analysis for Clubroot Resistance in Brassica oleracea.

Cited by 58 publications

References 36 publications

Genetic Analysis of Clubroot Resistance in Brassica Crops

Genetic Analysis of Clubroot Resistance in Brassica Crops

Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line

Evaluation of Cabbage- and Broccoli-genetic Resources for Resistance to Clubroot and Fusarium Wilt

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