A genetic linkage map of the cultivated strawberry (Fragaria × ananassa) and its comparison to the diploid Fragaria reference map

Sargent, Daniel J.; Fernández-Fernández, Felicidad; Ruiz-Roja, J. J.; Sutherland, B. G.; Passey, A. J.; Whitehouse, A. B.; Simpson, D. W.

doi:10.1007/s11032-009-9292-9

Cited by 71 publications

(75 citation statements)

References 25 publications

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“…Recent genetic maps have revealed macrosynteny between the diploid and octoploid genomes (Rousseau-Gueutin et al 2008;Sargent et al 2009;Spigler et al 2010), allowing the autosomal homeolog of the proto-sex chromosome of subdioecious F. virginiana to be identified . Our study takes advantage of this published work to begin to answer the overarching question: Is the sex-determining region of dioecious F. chiloensis syntenous with that of its sibling species, subdioecious F. virginiana?…”

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confidence: 99%

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

2010

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Separate sexes have evolved repeatedly from hermaphroditic ancestors in flowering plants, and thus select taxa can provide unparalleled insight into the evolutionary dynamics of sex chromosomes that are thought to be shared by plants and animals alike. Here we ask whether two octoploid sibling species of wild strawberry-one almost exclusively dioecious (males and females), Fragaria chiloensis, and one subdioecious (males, females, and hermaphrodites), F. virginiana-share the same sex-determining chromosome. We created a genetic map of the sex chromosome and its homeologs in F. chiloensis and assessed macrosynteny between it and published maps of the proto-sex chromosome of F. virginiana and the homeologous autosome of hermaphroditic diploid species. Segregation of male and female function in our F. chiloensis mapping population confirmed that linkage and dominance relations are similar to those in F. virginiana. However, identification of the molecular markers most tightly linked to the sexdetermining locus in the two octoploid species shows that, in both, this region maps to homeologues of chromosome 6 in diploid congeners, but is located at opposite ends of their respective chromosomes.

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Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

2010

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“…Therefore, all F. × ananassa maps were built on F1 progeny obtained from inter/intraspecific cross pollination (CP) of highly heterozygous cultivars (Haymes et al 1997;Lerceteau-Köhler et al 2003, 2012Rousseau-Gueutin et al 2008;Sargent et al 2009Sargent et al , 2012Goldberg et al 2010;Isobe et al 2012;Gaston et al 2013;van Dijk et al 2014;Mohamed 2014).…”

Section: Speciesmentioning

confidence: 99%

“…Since more abundant progeny carries a greater number of meiotic recombination events, thus, it determines the resolution and accuracy of the linkage map more precisely (Paterson 1996), larger segregating populations of over 100 individuals were introduced in the study on F. vesca (Koskela et al 2012), F. virginiana and F. chiloensis (Spigler et al 2008Goldberg et al 2010) as well as F. × ananassa (Lerceteau-Köhler et al 2003, 2012Rousseau-Gueutin et al 2008Weebadde et al 2008;Sargent et al 2009Isobe et al 2012;Gaston et al 2013;van Dijk et al 2014). Nevertheless, as some economic factors may limit the progeny number, the bin (selective) mapping appears to be a strategy of choice (Vision et al 2000).…”

Section: Speciesmentioning

confidence: 99%

“…Scaffolds of the maps were built based on RAPD (Random Amplified Polymorphic DNA) (Davis & Yu 1997;Sugimoto et al 2005), AFLP (Amplified Fragment Length Polymorphism) (Lerceteau-Köhler et al 2003Weebadde et al 2008;Sargent et al 2009), and ISSR (Inter-Simple Sequence Repeat) markers (Cekic et al 2001;Albani et al 2004). However, markers described above have many drawbacks, such as low reliability and insufficient level of polymorphism (RAPD), dominant inheritance (RAPD, AFLP) (Paran & Michelmore 1993), and labourintensive data scoring (ISSR) (Cekic et al 2001).…”

Section: Molecular Markers Used For Generation Of Map Skeletonmentioning

confidence: 99%

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The History Of Genome Mapping In Fragaria Spp.

Mohamed

Jęcz

Korbin

2014

Journal of Horticultural Research

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This overview summarizes the research programs devoted to mapping the genomes within Fragaria genus. A few genetic linkage maps of diploid and octoploid Fragaria species as well as impressive physical map of F. vesca were developed in the last decade and resulted in the collection of data useful for further fundamental and applied studies. The information concerning the rules for proper preparation of mapping population, the choice of markers useful for generating linkage map, the saturation of existing maps with new markers linked to economically important traits, as well as problems faced during mapping process are presented in this paper.

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“…For the purposes of conducting theoretical genetic analyses and efficient breeding in cultivated and wild octoploid strawberries, several linkage maps have recently been constructed using various types of DNA markers (Yamamoto et al 2003, Rousseau-Gueutin et al 2008, Spigler et al 2008, Weebadde et al 2008, Sargent et al 2009, Isobe et al 2013. However, mis-integration appeared to occur during the calculation process because many of the markers were mapped on multiple homologous regions across linkage groups.…”

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The Development of a Primed In Situ Hybridization Technique for Chromosome Labeling in Cultivated Strawberry (Fragaria×ananassa)

et al. 2016

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Summary Few studies have aimed to develop physical chromosome mapping methods for cultivated strawberry (Fragaria ananassa). Recently, linkage maps have been developed in this species using many single sequence repeat (SSR) markers. However, the accuracy of these needs to be evaluated because cultivated strawberries are allo-octoploids. This could be achieved by fluorescently labeling chromosomes that contain SSR markers on the same linkage group and observing whether other fluorescent signals can also be detected on the same chromosomes. The aim of this study was to develop a direct cycling primed in situ (C-PRINS) labeling technique for chromosome mapping of cultivated strawberry. To achieve this, we investigated the effect of the 1) maceration time for softening the root tips, 2) concentration of acetic acid for spreading chromosomes on the glass slide, 3) incubation time after slide preparation and before PRINS labeling, 4) number of PCR cycles for obtaining fluorescent signals, and 5) temperature accuracy for PCR. All of these factors were considered important for developing a chromosome labeling method. We found that a maceration time of 25 min was appropriate for obtaining many somatic cells with 56 chromosomes and that 45% acetic acid was effective for reducing the amount of damage to the chromosomes and obtaining clear chromosome images. A 72-h incubation time was appropriate for detecting chromosomes that had fluorescent signals, and the number of signals on the chromosomes increased with an increase in the number of PCR cycles. Finally, an aluminum tape treatment was effective for maintaining the PCR temperatures.

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A genetic linkage map of the cultivated strawberry (Fragaria × ananassa) and its comparison to the diploid Fragaria reference map

Cited by 71 publications

References 25 publications

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

The History Of Genome Mapping In Fragaria Spp.

The Development of a Primed <i>In Situ</i> Hybridization Technique for Chromosome Labeling in Cultivated Strawberry (<i>Fragaria</i>×<i>ananassa</i>)

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