A genetic linkage map of an inter-specific diploidFragariaBC1mapping population and its comparison with theFragariareference map (FV FN)

Nier, S.; Simpson, D. W.; Tobutt, K. R.

doi:10.1080/14620316.2006.11512118

Cited by 15 publications

(8 citation statements)

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“…For one locus (MdACAULIS4), the heterozygous products generated by sequencing from the FV × FN bin set seedlings were difficult to interpret unambiguously. This locus was mapped in the 815×903 BC diploid Fragaria population of Nier et al (2006) to confirm its map position, following the methods described above for mapping in Malus.…”

Section: Sequence Identification and Primer Designmentioning

confidence: 99%

“…"Totem" (F × T; Fernández-Fernández et al 2008), the diploid Fragaria reference bin mapping population (N=6) Fragaria vesca 815 × Fragaria nubicola 601 F 2 (FV × FN; Sargent et al 2008), and the Prunus reference bin mapping population (N=6) Prunus dulcis "Texas" × P. persica "Earlygold" F 2 (T × E; Howad et al 2005). An additional diploid Fragaria population-the 815×903BC population (N=93) of Nier et al (2006)was used to confirm the map position of one marker where the segregation scores produced using the FV × FN bin set were ambiguous. DNA was extracted from all progenies using the DNeasy Plant minikit (Qiagen) according the manufacturer's protocol and diluted 1:10 for use in PCR.…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

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Development of “universal” gene-specific markers from Malus spp. cDNA sequences, their mapping and use in synteny studies within Rosaceae

Marchese

Simpson

Howad

et al. 2008

Tree Genetics & Genomes

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The Rosaceae contains many economically valuable crop genera, including Malus (apple), Fragaria (strawberry), and Prunus (stone fruit). There has been increasing interest in the development of linkage maps for these species, with a view to marker-assisted selection to assist breeding programs and, recently, in the development of transferable markers to permit syntenic comparisons of maps of different rosaceous genera. In this investigation, a set of Malus cDNA sequences were downloaded from the European Molecular Biology Laboratory database. The sequences were aligned with homologous full-length Arabidopsis genomic DNA sequences to identify putative intron-exon junctions and conserved flanking exon sequences. Primer pairs were designed from the conserved exon sequences flanking predicted intron-exon junctions in the Malus cDNA sequences. These were used to amplify products by polymerase chain reaction from the parents of the Malus mapping progeny "Fiesta" × "Totem." Eleven loci, representing ten genes (39%), were polymorphic in the "Fiesta" × "Totem" population and mapped to seven Malus linkage groups. Transferability to other rosaceous genera was high, with primer pairs representing 85% of genes, amplifying products from Fragaria and primer pairs representing 85% of genes, amplifying products from Prunus genomic DNA. These primers were screened in the Fragaria and Prunus mapping bin sets and 38% of the genes were successfully located on both maps. Analysis of the markers mapped in more than one rosaceous genus revealed patterns of synteny between genera, while a comparison with the physical positions of homologous genes on the Arabidopsis genome revealed high sequence conservation but only fragmentary patterns of macrosynteny.

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Section: Sequence Identification and Primer Designmentioning

confidence: 99%

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Development of “universal” gene-specific markers from Malus spp. cDNA sequences, their mapping and use in synteny studies within Rosaceae

Marchese

Simpson

Howad

et al. 2008

Tree Genetics & Genomes

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“…Furthermore, this study showed high levels of colinearity between the homoeologous linkage groups in the cultivated strawberry and their corresponding diploid linkage groups, indicating that there were no major chromosomal rearrangements during the polyploidization events that gave rise to the octoploid progenitor species and ultimately to F.×ananassa. A parallel mapping study of a diploid wide cross between F. vesca and F. viridis also showed no major translocations or other rearrangements disrupting marker order between the species [54]. Indeed, it appears that there is much promise for comparative mapping within and across ploidy levels in Fragaria.…”

Section: Linkage Mapping and Genome Structurementioning

confidence: 97%

Applications of molecular markers in strawberry

Whitaker

2011

Journal of Berry Research

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This review examines current and potential applications of molecular markers in strawberry (Fragaria spp.). The most prevalent uses of markers in strawberry are for analyses of genetic diversity, genetic mapping, and cultivar identification. Cultivar identification is by far the most important use of markers to date for the protection of intellectual property as well as to confirm the identity of nursery stock through multiple cycles of asexual propagation. These applications have been accelerated by continuous discovery of simple sequence repeat markers and the development of multiplexed sets. Mapping studies have confirmed the allopolyploid genome structure of cultivated strawberry and shed light onto genetic events that punctuate its domestication. Though marker-trait associations have been identified for disease resistance, photoperiodic flowering, and sex expression, markerassisted selection in breeding is currently confined to a few entities in the private sector. Pedigree-based analysis holds promise for identifying additional marker-trait associations and simultaneously mining alleles in multiple genetic backgrounds. Meanwhile, the completion of the diploid strawberry genome sequence promises to accelerate candidate-gene approaches for marker discovery.

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“…In genetic mapping of diploid Fragaria plants, F2 population obtained from self-pollination or intercross of F1 has been used most frequently (Williamson et al 1995;Davis & Yu 1997;Deng & Davis 2001;Sargent et al 2004Sargent et al , 2006Sargent et al , 2007Sargent et al , 2008Sargent et al , 2011Ruiz-Rojas et al 2010;Illa et al 2011;Zorrilla-Fontanesi et al 2011b;Koskela et al 2012). However, some outline maps of F. vesca and F. viridis were elaborated on the basis of backcross population (BC), generated by crossing F1 individual(s) with inbred parent used in the initial cross (Nier et al 2006;Cekic et al 2001;Albani et al 2004). Also F3 population, obtained by crossing between F2 individuals, was successfully used for generating diploid Fragaria map .…”

Section: Speciesmentioning

confidence: 99%

“…The majority of segregating populations used for mapping genomes of Fragaria species contained 50 to 100 individuals Davis & Yu 1997;Haymes et al 1997;Sargent et al 2004Sargent et al , 2006Sargent et al , 2007Nier et al 2006;Rousseau-Gueutin et al 2008;Koskela et al 2012). Since more abundant progeny carries a greater number of meiotic recombination events, thus, it determines the resolution and accuracy of the linkage map more precisely (Paterson 1996), larger segregating populations of over 100 individuals were introduced in the study on F. vesca (Koskela et al 2012), F. virginiana and F. chiloensis (Spigler et al 2008Goldberg et al 2010) as well as F. × ananassa (Lerceteau-Köhler et al 2003, 2012Rousseau-Gueutin et al 2008Weebadde et al 2008;Sargent et al 2009Isobe et al 2012;Gaston et al 2013;van Dijk et al 2014).…”

Section: Speciesmentioning

confidence: 99%

The History Of Genome Mapping In Fragaria Spp.

Mohamed

Jęcz

Korbin

2014

Journal of Horticultural Research

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This overview summarizes the research programs devoted to mapping the genomes within Fragaria genus. A few genetic linkage maps of diploid and octoploid Fragaria species as well as impressive physical map of F. vesca were developed in the last decade and resulted in the collection of data useful for further fundamental and applied studies. The information concerning the rules for proper preparation of mapping population, the choice of markers useful for generating linkage map, the saturation of existing maps with new markers linked to economically important traits, as well as problems faced during mapping process are presented in this paper.

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A genetic linkage map of an inter-specific diploidFragariaBC₁mapping population and its comparison with theFragariareference map (FV FN)

Cited by 15 publications

References 0 publications

Development of “universal” gene-specific markers from Malus spp. cDNA sequences, their mapping and use in synteny studies within Rosaceae

Development of “universal” gene-specific markers from Malus spp. cDNA sequences, their mapping and use in synteny studies within Rosaceae

Applications of molecular markers in strawberry

The History Of Genome Mapping In Fragaria Spp.

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