A Genetic Linkage Map including Loci for Male Sterility, Sugars, and Ascorbic Acid in Melon

Park, Soon O.; Hwang, Hye Y.; Crosby, Kevin

doi:10.21273/jashs.134.1.67

Cited by 12 publications

(6 citation statements)

References 38 publications

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“…Melons have low to medium AA content, ranging from 10 to 35 mg/ 100 g FW depending on morphotypes and varieties (Davey et al, 2000;Fenech et al, 2018;. Despite its importance in global fruit production (over 30 million tons according to FAOSTAT), only a limited number of studies focus on the genetic manipulation of melon AA levels, and only a single AA-related QTL has been mapped on LG 5 in melon (Park et al, 2009). Therefore, the existence of different types of fruit together with the high diversity in AA content between cultivated varieties indicates that there is a great potential for improving AA levels in melon fruit.…”

Section: Discussionmentioning

confidence: 99%

Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit

Chatzopoulou

Sanmartín

Pateraki

et al. 2020

Plant Physiology and Biochemistry

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is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.

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Section: Discussionmentioning

confidence: 99%

Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit

Chatzopoulou

Sanmartín

Pateraki

et al. 2020

Plant Physiology and Biochemistry

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“…As mapping studies in melon have been conducted between elite, high-sugar cultivars, and wild accessions or landraces, favorable QTL alleles increasing SSC or SUC content beyond the already high levels of the former have not been described (Eduardo et al, 2007 ; Paris et al, 2008 ; Obando-Ulloa et al, 2009 ; Park et al, 2009 ; Harel-Beja et al, 2010 ; Castro et al, 2017 ). Our results were similar in that we identified just one QTL increasing SSC in one location ( sscqsc3.5_VAL11 ) in NIL SC3-5.…”

Section: Discussionmentioning

confidence: 99%

“…Considerable natural variation in sucrose content exists among the subspecies and groups, from the non-sweet melons (e.g., flexuosus group that are eaten as a vegetable) to very sweet (e.g., inodorus and cantalupensis groups) (Stepansky et al, 1999 ). Genetic and genomic resources developed in melon over the past several years (Argyris et al, 2015a ) together with different mapping populations have facilitated the development of a consensus genetic map (Diaz et al, 2011 ), and the identification of a number of quantitative trait loci (QTL) related to sugar accumulation (Monforte et al, 2004 ; Paris et al, 2008 ; Park et al, 2009 ; Harel-Beja et al, 2010 ; Perpiña et al, 2016 ; Castro et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

QTL Analyses in Multiple Populations Employed for the Fine Mapping and Identification of Candidate Genes at a Locus Affecting Sugar Accumulation in Melon (Cucumis melo L.)

et al. 2017

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Sugar content is the major determinant of both fruit quality and consumer acceptance in melon (Cucumis melo L), and is a primary target for crop improvement. Near-isogenic lines (NILs) derived from the intraspecific cross between a “Piel de Sapo” (PS) type and the exotic cultivar “Songwhan Charmi” (SC), and several populations generated from the cross of PS × Ames 24294 (“Trigonus”), a wild melon, were used to identify QTL related to sugar and organic acid composition. Seventy-eight QTL were detected across several locations and different years, with three important clusters related to sugar content located on chromosomes 4, 5, and 7. Two PS × SC NILs (SC5-1 and SC5-2) sharing a common genomic interval of 1.7 Mb at the top of chromosome 5 contained QTL reducing soluble solids content (SSC) and sucrose content by an average of 29 and 68%, respectively. This cluster collocated with QTL affecting sugar content identified in other studies in lines developed from the PS × SC cross and supported the presence of a stable consensus locus involved in sugar accumulation that we named SUCQSC5.1. QTL reducing soluble solids and sucrose content identified in the “Trigonus” mapping populations, as well as QTL identified in previous studies from other ssp. agrestis sources, collocated with SUCQSC5.1, suggesting that they may be allelic and implying a role in domestication. In subNILs derived from the PS × SC5-1 cross, SUCQSC5.1 reduced SSC and sucrose content by an average of 18 and 34%, respectively, and was fine-mapped to a 56.1 kb interval containing four genes. Expression analysis of the candidate genes in mature fruit showed differences between the subNILs with PS alleles that were “high” sugar and SC alleles of “low” sugar phenotypes for MELO3C014519, encoding a putative BEL1-like homeodomain protein. Sequence differences in the gene predicted to affect protein function were restricted to SC and other ssp. agrestis cultivar groups. These results provide the basis for further investigation of genes affecting sugar accumulation in melon.

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“…This crop has a high global production (∼31 million tons; FAOSTAT) and it is also amongst the highest productions in the European Union (∼3 million tons; Eurostat, 2017). A single QTL for ascorbate has been mapped on LG 5 using different populations (Sinclair et al, 2006; Park et al, 2009). However, low reproducible RAPD markers were used in these studies, hampering their application in breeding programs.…”

Section: Approaches To Increase Ascorbate In Fruitsmentioning

confidence: 99%

Vitamin C Content in Fruits: Biosynthesis and Regulation

et al. 2019

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Throughout evolution, a number of animals including humans have lost the ability to synthesize ascorbic acid (ascorbate, vitamin C), an essential molecule in the physiology of animals and plants. In addition to its main role as an antioxidant and cofactor in redox reactions, recent reports have shown an important role of ascorbate in the activation of epigenetic mechanisms controlling cell differentiation, dysregulation of which can lead to the development of certain types of cancer. Although fruits and vegetables constitute the main source of ascorbate in the human diet, rising its content has not been a major breeding goal, despite the large inter- and intraspecific variation in ascorbate content in fruit crops. Nowadays, there is an increasing interest to boost ascorbate content, not only to improve fruit quality but also to generate crops with elevated stress tolerance. Several attempts to increase ascorbate in fruits have achieved fairly good results but, in some cases, detrimental effects in fruit development also occur, likely due to the interaction between the biosynthesis of ascorbate and components of the cell wall. Plants synthesize ascorbate de novo mainly through the Smirnoff-Wheeler pathway, the dominant pathway in photosynthetic tissues. Two intermediates of the Smirnoff-Wheeler pathway, GDP-D-mannose and GDP-L-galactose, are also precursors of the non-cellulosic components of the plant cell wall. Therefore, a better understanding of ascorbate biosynthesis and regulation is essential for generation of improved fruits without developmental side effects. This is likely to involve a yet unknown tight regulation enabling plant growth and development, without impairing the cell redox state modulated by ascorbate pool. In certain fruits and developmental conditions, an alternative pathway from D-galacturonate might be also relevant. We here review the regulation of ascorbate synthesis, its close connection with the cell wall, as well as different strategies to increase its content in plants, with a special focus on fruits.

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A Genetic Linkage Map including Loci for Male Sterility, Sugars, and Ascorbic Acid in Melon

Cited by 12 publications

References 38 publications

Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit

Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit

QTL Analyses in Multiple Populations Employed for the Fine Mapping and Identification of Candidate Genes at a Locus Affecting Sugar Accumulation in Melon (Cucumis melo L.)

Vitamin C Content in Fruits: Biosynthesis and Regulation

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