A genetic assessment of parentage in a natural population of dollar sunfish (<i>Lepomis marginatus</i>) based on microsatellite markers

Mackiewicz, Mark; Fletcher, Dean E.; Wilkins, Selwyn; DeWoody, J. Andrew; Avise, John C.

doi:10.1046/j.1365-294x.2002.01577.x

Cited by 36 publications

(20 citation statements)

References 35 publications

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“…The program PARENT-AGE identiWed 16 new mutations among the 682 young surveyed at four loci (5,456 alleles in total), thus yielding an estimated mutation rate of 2.9 £ 10 ¡3 . This rate is similar to those detected in various other microsatellite studies (Weber and Wong 1993;Hancock 1999;Mackiewicz et al 2002). It should be also noted that this estimate encompasses any mis-scoring of alleles (i.e., we did not distinguish de novo mutation from possible mis-scoring for the aberrant alleles in our analysis).…”

Section: Microsatellite Markers and Genetic Population Summarysupporting

confidence: 90%

Multiple paternity and extra-group fertilizations in a natural population of California grunion (Leuresthes tenuis), a beach-spawning marine fish

Byrne

Avise

2009

Mar Biol

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Although individuals in many Wsh species move to shallow waters to spawn, the California grunion (Leuresthes tenuis) is almost unique in its constitutive display of synchronous full-emergence beach spawning. During a spawning event, Wsh ride large waves onshore to spawn on beach land, where their eggs incubate terrestrially. Here, we employ molecular markers to ascertain how this unusual reproductive behavior impacts genetic parentage. We developed and utilized four highly polymorphic microsatellite markers to assess maternal and paternal contributions in a total of 682 progeny from 17 nests of a natural population of L. tenuis. Alleles deduced to be of paternal origin in progeny were used to determine the minimum number of sires per nest and to estimate the true number of sires per nest via Bayesian analysis. We document the following: (a) no instances of multiple maternity for progeny within a nest; (b) a high frequency of nests (88%) with multiple paternity; and (c) an appreciable fraction of nests (18%) in which the estimated number of genetic sires (as many as nine) proved to be greater than the observed number of male attendants, thus implicating occasional extra-group fertilization events. From these and other observations, we also conclude that spawning behavior in grunions may involve site choice but not explicit mate choice. In addition to providing the Wrst analysis of molecular parentage in a beach-spawning Wsh, we compare our Wndings to those reported previously for a beach-spawning arthropod, and we discuss the forces that may be maintaining this peculiar reproductive behavior.

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Section: Microsatellite Markers and Genetic Population Summarysupporting

confidence: 90%

Multiple paternity and extra-group fertilizations in a natural population of California grunion (Leuresthes tenuis), a beach-spawning marine fish

Byrne

Avise

2009

Mar Biol

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“…We ran ten replicate simulations for three mutation rates (1 9 10 -3 , 1 9 10 -4 , 1 9 10 -5 ) and N e values of 50, 100, 250, 500, and 1,000, 5,000, and 10,000 to fully encompass the empirical longterm estimates of N e . Mutation rate estimates for microsatellites have a large degree of uncertainty (Ellegren 2004) but the typical range observed by most studies to date has been 1 9 10 -3 -1 9 10 -5 (Jones et al 1999;Shimoda et al 1999;MacKiewicz et al 2002;Steinberg et al 2002;Ellegren 2004;Yue et al 2007). Simulations were started with minimal variation and we recorded the final H S after 2,500 generations.…”

Section: Above-barrier Populationsmentioning

confidence: 99%

Genetic variation and effective population size in isolated populations of coastal cutthroat trout

Whiteley

Hastings²,

Wenburg

et al. 2010

Conserv Genet

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Following glacial recession in southeast Alaska, waterfalls created by isostatic rebound have isolated numerous replicate populations of coastal cutthroat trout (Oncorhynchus clarkii clarkii) in short coastal streams. These replicate isolated populations offer an unusual opportunity to examine factors associated with the maintenance of genetic diversity. We used eight microsatellites to examine genetic variation within and differentiation among 12 population pairs sampled from above and below these natural migration barriers. Geological evidence indicated that the above-barrier populations have been isolated for 8,000-12,500 years. Genetic differentiation among below-barrier populations (F ST = 0.10, 95% C.I. 0.08-0.12) was similar to a previous study of more southern populations of this species. Above-barrier populations were highly differentiated from adjacent below-barrier populations (mean pairwise F ST = 0.28; SD 0.18) and multiple lines of evidence were consistent with asymmetric downstream gene flow that varied among streams. Each above-barrier population had reduced within-population genetic variation when compared to the adjacent belowbarrier population. Within-population genetic diversity was significantly correlated with the amount of available habitat in above-barrier sites. Increased genetic differentiation of above-barrier populations with lower genetic diversity suggests that genetic drift has been the primary cause of genetic divergence. Long-term estimates of N e based on loss of heterozygosity over the time since isolation were large (3,170; range 1,077-7,606) and established an upper limit for N e if drift were the only evolutionary process responsible for loss of genetic diversity. However, it is likely that a combination of mutation, selection, and gene flow have also contributed to the genetic diversity of above-barrier populations. Contemporary above-barrier N e estimates were much smaller than long-term N e estimates, not correlated with withinpopulation genetic diversity, and not consistent with the amount of genetic variation retained, given the approximate 10,000-year period of isolation. The populations isolated by waterfalls in this study that occur in larger stream networks have retained substantial genetic variation, which suggests that the amount of habitat in headwater streams is an important consideration for maintaining the evolutionary potential of isolated populations.

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“…Several recent studies using microsatellite DNA markers for paternity analysis have focused on reproductive behavior and variability in male reproductive success (22,23). The parents of an individual also can be identified based on likelihood statistics using hypervariable microsatellite markers.…”

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confidence: 99%

Larval dispersal connects fish populations in a network of marine protected areas

Planes

Jones

Thorrold

2009

Proc. Natl. Acad. Sci. U.S.A.

421

393

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Networks of no-take marine protected areas (MPAs) have been widely advocated for the conservation of marine biodiversity. But for MPA networks to be successful in protecting marine populations, individual MPAs must be self-sustaining or adequately connected to other MPAs via dispersal. For marine species with a dispersive larval stage, populations within MPAs require either the return of settlement-stage larvae to their natal reserve or connectivity among reserves at the spatial scales at which MPA networks are implemented. To date, larvae have not been tracked when dispersing from one MPA to another, and the relative magnitude of local retention and connectivity among MPAs remains unknown. Here we use DNA parentage analysis to provide the first direct estimates of connectivity of a marine fish, the orange clownfish (Amphiprion percula), in a proposed network of marine reserves in Kimbe Bay, Papua New Guinea. Approximately 40% of A. percula larvae settling into anemones in an island MPA at 2 different times were derived from parents resident in the reserve. We also located juveniles spawned by Kimbe Island residents that had dispersed as far as 35 km to other proposed MPAs, the longest distance that marine larvae have been directly tracked. These dispersers accounted for up to 10% of the recruitment in the adjacent MPAs. Our findings suggest that MPA networks can function to sustain resident populations both by local replenishment and through larval dispersal from other reserves. More generally, DNA parentage analysis provides a direct method for measuring larval dispersal for other marine organisms.Amphiprion percula ͉ connectivity ͉ parentage analyses ͉ self-recruitment

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A genetic assessment of parentage in a natural population of dollar sunfish (Lepomis marginatus) based on microsatellite markers

Cited by 36 publications

References 35 publications

Multiple paternity and extra-group fertilizations in a natural population of California grunion (Leuresthes tenuis), a beach-spawning marine fish

Multiple paternity and extra-group fertilizations in a natural population of California grunion (Leuresthes tenuis), a beach-spawning marine fish

Genetic variation and effective population size in isolated populations of coastal cutthroat trout

Larval dispersal connects fish populations in a network of marine protected areas

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