A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion

Abstract

Screen identifies genes that affect tumor behavior in a wide variety of ways A functionally validated network of invasionsuppressor genes was generated Loss of cohesin complex function can promote individual or collective cell invasionThe fly pupal notum is an excellent in vivo system to study tumor progression

Generation and live imaging of tumors with specific genotypes in the living fly pupa

“…Tumors lacking lgl generate large, partially multi-layered tumors, and present a low-level invasive phenotype, representing an ideal scenario for screening for enhancers or suppressors of tumor progression. We characterized the cohesin complex subunits as tumor suppressor genes using this model (See expected outcomes and Canales Coutiño et al., 2020 ). For a detailed rationale of our experimental design and for the results of the 497 different genes we studied using this model, see Canales Coutiño et al.…”

“…We characterized the cohesin complex subunits as tumor suppressor genes using this model (See expected outcomes and Canales Coutiño et al., 2020 ). For a detailed rationale of our experimental design and for the results of the 497 different genes we studied using this model, see Canales Coutiño et al. (2020) .…”

“…Error bars represent ± SEM. (B) and (C) reprinted with permission from Canales Coutiño et al. (2020) .…”

“…Thus, this model allows the study of conserved genes that enhance or suppress epithelial tumor progression. For complete details on the use and execution of this protocol, please refer to Canales Coutiño et al. (2020) .…”

“…For complete details on the use and execution of this protocol, please refer to Canales Coutiño et al. (2020) .…”