A generalized approach to thermodynamic properties of biomolecules for use in bioseparation process design

Ahamed, Tangir; Ottens, Marcel; Nfor, Beckley K.; Dedem, G.W.K. van; Wielen, Luuk A.M. van der

doi:10.1016/j.fluid.2005.12.011

Cited by 29 publications

(18 citation statements)

References 94 publications

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“…A good example of a hybrid approach to protein purification is that proposed by Ahamed et al 68 The approach makes use of a combination of HTE and modeling tools for rationally synthesizing and designing a protein purification process, as depicted in Fig. 2.…”

Section: Hybrid Methodsmentioning

confidence: 99%

“…69 Thermodynamic generalization was proposed as a way of interrelating the relevant thermodynamic properties of proteins in order to reduce the number model parameters and hence experimentation required for modeling the purification units involved. 68 A rational synthesis methodology is required for rapidly generating alternative process options and for screening them to select the best one. Once the best process has been selected, and prior to its detailed design, HTE based on HTS techniques are used to optimize individual unit operations within the flowsheet.…”

Section: Hybrid Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Design strategies for integrated protein purification processes: challenges, progress and outlook

Nfor

Ahamed

Dedem

et al. 2007

J of Chemical Tech & Biotech

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The key to successful and efficient protein purification is the selection of the most appropriate purification techniques and their combination in a logical way to obtain the desired purification in the minimum number of steps. However, the rationalization of protein purification process development is faced with a number of challenges. In this paper, the challenges in protein purification process development are captured. The state-ofthe-art in protein purification process synthesis and design is reviewed and the strengths and weaknesses of the current strategies highlighted. Finally, views on the future directions of protein purification process development are presented.

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Section: Hybrid Methodsmentioning

confidence: 99%

Section: Hybrid Methodsmentioning

confidence: 99%

Design strategies for integrated protein purification processes: challenges, progress and outlook

Nfor

Ahamed

Dedem

et al. 2007

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ion exchange chromatography (IEC) is often used as the polishing step following Protein A capture step to remove mAb variants, HCP, and leached Protein A . It utilizes the variation in the net charge of the proteins with the change in pH for protein separation . Adjusting the ionic strength and pH in the mobile phase can modulate protein binding and elution.…”

Section: Introductionmentioning

confidence: 99%

“…24 It utilizes the variation in the net charge of the proteins with the change in pH for protein separation. 25 Adjusting the ionic strength and pH in the mobile phase can modulate protein binding and elution. Salt gradients at fixed pH value are often used to elute retained proteins from the IEC.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of differences between dual salt‐pH gradient elution and mono gradient elution using a thermodynamic model: Simultaneous separation of six monoclonal antibody charge and size variants on preparative‐scale ion exchange chromatographic resin

Lee

Jöhnck

Frech

2018

Biotechnology Progress

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The efficiencies of mono gradient elution and dual salt-pH gradient elution for separation of six mAb charge and size variants on a preparative-scale ion exchange chromatographic resin are compared in this study. Results showed that opposite dual salt-pH gradient elution with increasing pH gradient and simultaneously decreasing salt gradient is best suited for the separation of these mAb charge and size variants on Eshmuno CPX. Besides giving high binding capacity, this type of opposite dual salt-pH gradient also provides better resolved mAb variant peaks and lower conductivity in the elution pools compared to single pH or salt gradients. To have a mechanistic understanding of the differences in mAb variants retention behaviors of mono pH gradient, parallel dual salt-pH gradient, and opposite dual salt-pH gradient, a linear gradient elution model was used. After determining the model parameters using the linear gradient elution model, 2D plots were used to show the pH and salt dependencies of the reciprocals of distribution coefficient, equilibrium constant, and effective ionic capacity of the mAb variants in these gradient elution systems. Comparison of the 2D plots indicated that the advantage of opposite dual salt-pH gradient system with increasing pH gradient and simultaneously decreasing salt gradient is the noncontinuous increased acceleration of protein migration. Furthermore, the fitted model parameters can be used for the prediction and optimization of mAb variants separation in dual salt-pH gradient and step elution. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:973-986, 2018.

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“…Therefore, systematic process development based on identification of justified optima is essential for efficient utilization of time and resources. Nfor et al (2009) identified four types of process synthesis strategies applicable to chemical industries: heuristics or knowledge-based strategies (Cziner et al, 2005), optimization-based strategies (Steimel et al, 2013;Grossmann and Daichendt, 1996), high-throughput experimentation strategies (Bhambure et al, 2011;Schuldt and Schembecker, 2013) and a combination of the aforementioned strategies (Ahamed et al, 2006). Each approach has strengths and weaknesses as discussed elsewhere (Nfor et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of hydrometallurgical processes for valorization of secondary raw materials using ant colony optimization and key performance indicators

2015

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An algorithm-based method for synthesis of hydrometallurgical processes using limited amounts of experimental data is presented. The method enables simultaneous selection and sequencing of unit operations and optimization of operating parameters. An ant colony optimization (ACO) based algorithm is used to identify the most economic process alternative in an iterative manner. Key performance indicators are used for comparison of candidate processes: a purification performance index measures purity improvement and a separation cost indicator is used as an objective function in process optimization. Computational times were reduced significantly with the suggested method compared to an algorithm which evaluates all the possible process options. The practical applicability of the method to hydrometallurgy is demonstrated by investigating zinc recovery from argon oxygen decarburization dust with two alternative leaching methods and recovery of lanthanides from nickel metal hydride (NiMH) batteries. In the first zinc recovery process, 150 min normal batch leaching with 0.5 M H 2 SO 4 is used, and in the other one 270 min batch leaching with H 2 SO 4 is done by controlling the pH (N 3.0). In both cases the leachate is extracted with D2EHPA at pH 4.27, and stripped with circulating solution from zinc electrolysis. For lanthanides recovery the algorithm suggested a process in which the raw material is leached with 1.3 M HCl, the leachate is extracted with D2EHPA at pH 2.2, organic phase is stripped with 2.0 M HCl and 99% pure Ln-oxalates are precipitated with oxalic acid at pH 0.6. Compared to previously suggested process for the same raw material, the algorithm suggests operating the leaching step such that higher selectivity is achieved by sacrificing some yield. AbbreviationsACO ant colony optimization AOD argon oxygen decarburization CPU central processing unit EDR energy dissipation rate IRR internal rate of return KPI key performance indicator PPI purification performance index SCI separation cost indicator Notation A flow rate, m 3 /s c i concentration of contaminants, kg/m 3 E concentration of extractant, m 3 /m 3 K cost, item of expenses, €/kg k L specific cost of a leaching step, €/kg k pur,l specific cost of a purification step, €/kg L probability M number of components in a chemical system N number of ants in a colony n number of process steps P number of discrete values of operating parameters SL solvent loss in solvent extraction T concentration of a target metal in the system, kg/m 3 t b batch time in leaching, s U number of unit operations V L volume of leaching vessel, m 3 x purity Y yieldGreek symbols α the degree of importance of the pheromones ξ parameter used to control the scale of the global updating of the pheromone τ l,u,p amount of pheromone in a cell ρ pheromone decay factor Hydrometallurgy 153 (2015) 121-133

show abstract

A generalized approach to thermodynamic properties of biomolecules for use in bioseparation process design

Cited by 29 publications

References 94 publications

Design strategies for integrated protein purification processes: challenges, progress and outlook

Design strategies for integrated protein purification processes: challenges, progress and outlook

Evaluation of differences between dual salt‐pH gradient elution and mono gradient elution using a thermodynamic model: Simultaneous separation of six monoclonal antibody charge and size variants on preparative‐scale ion exchange chromatographic resin

Synthesis of hydrometallurgical processes for valorization of secondary raw materials using ant colony optimization and key performance indicators

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