A General Sight about Linking PARP-1 and NFKB1 Variations to the Inflammatory Events

MiRNAs and NFKB1 are well-known immune response and inflammation regulators. MiRNA gene polymorphisms may affect miRNA biogenesis and function and, may thus, lead to changes in the expression of hundreds of genes such as NFKB1. The aim of this study was to investigate the association of Behcet's disease (BD) with NFKB1 rs28362491, pre-miRNA-146a rs2910164, and pre-miRNA-499 rs3746444 polymorphisms, as well as the analysis of their single and combined effects on its susceptibility in a Turkish population. These polymorphisms were analyzed by using the polymerase chain reaction-restriction fragment length polymorphism method in 100 BD patients and 145 healthy control subjects. The results were analyzed statistically using Pearson chi-square (χ(2)) test and Fisher's exact test (two sided). According to genotype analysis, the frequencies of ins/ins genotype and ins allele of rs28362491 were considerably higher in BD patients. Also, miRNA-499 rs3746444 homozygous (TT) genotypes exibited a significantly higher risk in patients with BD (odds ratios [OR]=3.0, 95% confidence intervals [95% CI]=1.284-7.007, p=0.017). Moreover, the frequency of T allele of rs3746444 was a risk factor for BD (OR=1.562, 95% CI=1.087-2.24, p=0.015). In addition, significant differences were found between the groups concerning miRNA-146a rs2910164 polymorphism. Homozygous CC genotype and C allele of rs2910164 polymorphism were found to be protective factors against BD. The results of the combined genotype analysis showed no notable differences between the multiple comparisons of rs28362491-rs2910164 and of rs28362491-rs3746444 in patients and control groups. Our data demonstrate that homozygous CC genotype and C allele of rs2910164 polymorphism are protective factors against BD, but rs3746444 and rs28362491 polymorphisms in miRNA-499 and in NFKB1 promoter are involved in the genetic susceptibility of BD. In addition, TT and ins/ins genotypes may influence certain proinflammatory cytokines and, may thus, play a role in the pathogenesis of BD.

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Association of Pre-miRNA-499 rs3746444 and Pre-miRNA-146a rs2910164 Polymorphisms and Susceptibility to Behcet's Disease

Oner

Yenmiş

Tombultürk

et al. 2015

Genetic Testing and Molecular Biomarkers

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Study of the Relationship of Polymorphisms of NF-kB1 miR-34b/c Genes with Risk of Severe Oli Gozoospermia in a Sample of Iraqi Patients

Alhassan,

Abdulhassan,

Al-Thwani

2023

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Infertility is a disease that affects reproduction and is one of the most common marital problems, which is considered a stigma in our society. It is considered a big problem in countries, states and developing countries (third world countries) because of the modern lifestyle. This type of study is looking for the causes of this problem. This study was conducted from January to June 20 in the Infertility Unit in Baghdad, Iraq, on 100 men; 60 of the women were infertile, and 40 were healthy and not infertile. Polymerase chain reaction and restriction fragment length polymorphism analyses were used for genotyping the rs 4938723 and rs28362491 SNPs.This study aims to study the genes NF-KB1 and Mir34b/c, to measure the antibodies to sperm, and to know the immune role of interleukin-6 in infertile and non-sterile men. The genes appeared with desirable and natural results. Where the proportion of genes was (P≤0.05), these results were the lowest compared to previous studies on the same genes. Keywords: DNA, Genotype, PCR, Blood,

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A General Sight about Linking PARP-1 and NFKB1 Variations to the Inflammatory Events

Cited by 2 publications

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Association of Pre-miRNA-499 rs3746444 and Pre-miRNA-146a rs2910164 Polymorphisms and Susceptibility to Behcet's Disease

Association of Pre-miRNA-499 rs3746444 and Pre-miRNA-146a rs2910164 Polymorphisms and Susceptibility to Behcet's Disease

Study of the Relationship of Polymorphisms of NF-kB1 miR-34b/c Genes with Risk of Severe Oli Gozoospermia in a Sample of Iraqi Patients

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