A general primer GP5+/GP6(+)-mediated PCR-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings

Abstract

Two cocktails of digoxigenin-labeled human papillomavirus (HPV) type-specific oligonucleotide probes and an enzyme immunoassay (EIA) were used as a basis to develop a group-specific detection method for 14 high-risk (

Human papillomavirus‐negative ileostomal chronic papillomatous dermatitis

“…Although clinical and morphologic similarities exist between our patient's lesion and that previously reported by Wieland et al, we used the same PCR protocols and yielded no HPV. [9][10][11] In addition, we were unable to detect any viral particles by TEM or immunohistochemistry. Other similar ileostomy verrucous lesions reported have also been unsuccessfully linked to a viral etiology.…”

“…TEM revealed good preservation of the specimen that was devoid of viral particles. A portion of normal ileum and a portion of the papillomatous lesion were studied with PCR to detect a very broad spectrum of cutaneous and genital HPV, [9][10][11] and none of the samples contained HPV-DNA.…”

Anal carcinoma in human immunodeficiency virus-positive men: results of a prospective study from Germany

“…18 For HPV typing, internal, biotinylated A6 ⁄A8 PCR products were hybridized with 36 type-specific digoxigenin-labelled oligonucleotide probes in an enzyme immunoassay. 19 Anal cancer biopsies were additionally HPV typed using a bead-based multiplex genotyping assay as described before. 17 HPV types 16, 18, 26, 31, 33, 35, 39, 45, 51-53, 56, 58, 59, 66, 68, 73 and 82 were considered as high-risk a-HPV types.…”

High level HPV‐16 E7 oncoprotein expression correlates with reduced pRb‐levels in cervical biopsies

“…Formalin-fixed paraffin-embedded tissues were processed with the QIAamp Tissue Kit according to the instructions of the manufacturer (Qiagen). Total cellular DNA was used in the GP5+/GP6+ general primer PCR, and the amplicons were used for HPV typing with the use of an enzyme immunoassay with different HPV type-specific oligonucleotides (19).…”