A general excimer signaling approach for aptamer sensors

Wu, Cuichen; Yan, Ling; Wang, Chunming; Lin, Haoxue; Wang, Chi; Chen, Xi; Yang, Chaoyong

doi:10.1016/j.bios.2010.02.030

Cited by 85 publications

(41 citation statements)

References 39 publications

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“…[20][21][22] These advantages make aptamers promising ligands for medical and pharmaceutical research, drug development, diagnosis and therapy. [23][24][25][26][27][28] In this study, using recombinant Shp2, we have identified two classes of single-stranded DNA aptamers that selectively bind to Shp2 with high affinity. Structural studies on one of the aptamer sequences, HJ24, revealed a parallel G-quadruplex as the core binding domain.…”

Section: Introductionmentioning

confidence: 99%

A G‐Quadruplex Aptamer Inhibits the Phosphatase Activity of Oncogenic Protein Shp2 in vitro

Liu

et al. 2011

ChemBioChem

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Shp2 is a member of the protein tyrosine phosphatase (PTP) family, which regulates a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Using a recombinant Shp2-GST protein as the target and GST as a counter target, we have identified two classes of single-stranded DNA aptamers that selectively bind to Shp2 with a K(d) in the nanomolar range. Structural studies of the most abundant sequence in the enriched library, HJ24, revealed a parallel G-quadruplex as the core binding domain. Furthermore, this aptamer was found to be an effective inhibitor of Shp2 phosphatase, an effect which was readily reversed by using the cDNA of HJ24. In view of these characteristics, this aptamer has the potential to be used for further development of Shp2 assays and therapeutics for the treatment of Shp2-dependent cancers and other diseases.

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Section: Introductionmentioning

confidence: 99%

A G‐Quadruplex Aptamer Inhibits the Phosphatase Activity of Oncogenic Protein Shp2 in vitro

Liu

et al. 2011

ChemBioChem

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“…92 Pyrene excimer formation has also been used in this design. 93 A variation of this is to immobilize each fragment on a AuNP to produce analyte-induced assembly ( Figure 2F). 94 In addition, split aptamers have been attached on fluid liposome surfaces.…”

Section: Split Aptamer Sensorsmentioning

confidence: 99%

Aptamer-based biosensors for biomedical diagnostics

Zhou

Huang

Ding

et al. 2014

Analyst

454

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Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any molecule of choice, can be readily modified at arbitrary positions and they possess predictable secondary structures, this platform technology shows great promise in biosensor development. Over the past two decades, more than one thousand papers have been published on aptamer-based biosensors. Given this progress, the application of aptamer technology in biomedical diagnosis is still in a quite preliminary stage. Most previous work involves only a few model aptamers to demonstrate the sensing concept with limited biomedical impact. This Critical Review aims to summarize progresses that might enable practical applications of aptamer for biological samples. First, general sensing strategies based on the unique properties of aptamers are summarized. Each strategy can be coupled to various signaling methods. Among these, a few detection methods including fluorescence lifetime, flow cytometry, upconverting nanoparticles, nanoflare technology, magnetic resonance imaging, electronic aptamer-based sensors, and lateral flow devices have been discussed in more detail since they are more likely to work in a complex sample matrix. The current limitations of this field include the lack of high quality aptamers for clinically important targets. In addition, the aptamer technology has to be extensively tested in a clinical sample matrix to establish reliability and accuracy. Future directions are also speculated to overcome these challenges.3

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“…Table S2 † provides a summarized comparison of the previous reports of aptamer excimer probe based assays for other target molecules. [36][37][38][39][41][42][43] For the aptamer having low binding affinity (e.g. the aptamers for cocaine, ATP, and potassium ions), the obtained detection limits are high.…”

Section: Detection Of Igementioning

confidence: 99%

“…the aptamers for cocaine, ATP, and potassium ions), the obtained detection limits are high. 38,39,41 The aptamer with high binding affinity allowed aptamer excimer based assays to have higher sensitivity (e.g. the assays for PDGF-BB and thrombin).…”

Section: Detection Of Igementioning

confidence: 99%

“…The combination of the aptamer and pyrene excimer provides an aptamer excimer probe for rapid detection of targets based on target-binding induced structure switching of the aptamer. [36][37][38][39][40][41][42][43] Yang et al reported an excimer aptamer probe to detect platelet-derived growth factor BB (PDGF-BB). 36 Pyrene was conjugated on each end of the aptamer against PDGF-BB.…”

Section: Introductionmentioning

confidence: 99%

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Rapid fluorescence detection of immunoglobulin E using an aptamer switch based on a binding-induced pyrene excimer

Bai

Zhao

2017

Anal. Methods

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Immunoglobulin E (IgE) is an important immunoglobulin related to allergic reactions and some diseases such as atopic dermatitis, allergic asthma, etc. Sensitive and rapid detection of IgE is desired for disease diagnosis and therapeutics. Taking advantage of a binding-induced pyrene excimer, we developed a sensitive fluorescent aptamer switch for IgE sensing by using a pyrene-labeled aptamer. The aptamer having four base pairs at the stem was labeled with a single pyrene molecule at the 3 0 end and 5' end and used as an affinity probe. The binding of IgE brought the pyrene moiety at each terminal of the aptamer into proximity, generating an excimer and showing a distinct fluorescence peak. The measurement of the fluorescence peak of the excimer allowed the sensitive detection of IgE. We optimized the experimental conditions such as temperature, incubation time, and metal cations. The detection limit for IgE was about 1.6 nM. This assay displayed good specificity and allowed for the detection of IgE spiked in a diluted human serum sample. This fluorescent aptamer sensor has potential for its application in rapid sensing of IgE.

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A general excimer signaling approach for aptamer sensors

Cited by 85 publications

References 39 publications

A G‐Quadruplex Aptamer Inhibits the Phosphatase Activity of Oncogenic Protein Shp2 in vitro

A G‐Quadruplex Aptamer Inhibits the Phosphatase Activity of Oncogenic Protein Shp2 in vitro

Aptamer-based biosensors for biomedical diagnostics

Rapid fluorescence detection of immunoglobulin E using an aptamer switch based on a binding-induced pyrene excimer

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