A General Classification of New World Leishmania Using Numerical Zymotaxonomy

Epidemiology of leishmaniasis in Ecuador: current status of knowledge - A review

“…L. major-like parasite had also been reported from Brazil, Paraguay, Venezuela, and Mexico (Cupolillo et al 1994, Yamasaki et al 1994 suggesting that some of these populations may have been imported into the Americas (Momen et al 1993). The nucleotide sequences of cytochrome b of L. major-like isolated from the Ecuadorian Andes and the WHO reference strain L. major had a 99.9% homology (Luyo-Acero et al 2004).…”

Efeito de uma formulação hidrofílica de paromomicina tópica na leishmaniose cutânea em pacientes com contra-indicações de tratamento com antimonial pentavalente

“…Foram realizadas a intradermorreação de Montenegro e a cultura do aspirado de lesões cutâneas e de linfonodos infartados, segundo os métodos previamente descritos 19 . Os parasitas isolados foram identificados utilizando-se a eletroforese de enzimas conforme metodologia previamente descrita 6 . Também foram realizadas biópsias da borda da lesão para extrair DNA para realizar a reação em cadeia pela polimerase (PCR), tendo como alvo de amplificação a região conservada de 120 pares de bases do k-DNA do parasito.…”

Characterization of Leishmania infantum species in dogs from the urban area of Cuiabá, State of Mato Grosso, Brazil

“…Studies conducted by Cupolillo et al 5 classified strains of Leishmania according to species as belonging to the New World and the Old World. Species characterization is fundamental for understanding the biology of the parasite and, although in Brazil the main species is Leishmania infantum 5 , Tolezano et al 6 proved that infection in two dogs with clinical VL, from Araçatuba, SP, had been caused by Leishmania amazonensis.…”

“…The tests followed a previously described protocol by Cupolillo et al 5 and the enzymes tested were: 6PGDH (6-phosphogluco-dehydrogenase, E.C. 1.1.1.43), G6PDH (glucose-6-phosphate dehydrogenase, E.C.1.1.…”