2013
DOI: 10.1016/j.micron.2012.07.006
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A general approach for the microrheology of cancer cells by atomic force microscopy

Abstract: The determination of the viscoelastic properties of cells by atomic force microscopy (AFM) is mainly realized by looking at the relaxation of the force when a constant position of the AFM head is maintained or at the evolution of the indentation when a constant force is maintained. In both cases the analysis rests on the hypothesis that the motion of the probe before the relaxation step is realized in a time which is much smaller than the characteristic relaxation time of the material. In this paper we carry o… Show more

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Cited by 23 publications
(24 citation statements)
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References 31 publications
(35 reference statements)
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“…However, in an actual experiment, the maximum loading speed is limited by hydrodynamic and inertial effects and step-loading conditions are impossible to implement. The fit of the total ramp-hold curve 49 or introduction of the ramp correction factor should be implemented for proper analysis 50, 51 . Difficulties in interpretation may also arise at long relaxation times due to thermal drift in the instrument 52 and from active cell responses to the applied forces 53 .…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, in an actual experiment, the maximum loading speed is limited by hydrodynamic and inertial effects and step-loading conditions are impossible to implement. The fit of the total ramp-hold curve 49 or introduction of the ramp correction factor should be implemented for proper analysis 50, 51 . Difficulties in interpretation may also arise at long relaxation times due to thermal drift in the instrument 52 and from active cell responses to the applied forces 53 .…”
Section: Discussionmentioning
confidence: 99%
“…Generalized Maxwell model with two or more relaxation times increased the quality of the fit relative to the single relaxation time like in SLS model 44, 47, 50 . But it is not completely clear if it is really capturing some physical phenomena or quality of the fit increased just due to the introduction of additional fitting parameters.…”
Section: Discussionmentioning
confidence: 99%
“…It is now widely accepted that G(ω) follows a weak power‐law Gfalse(ωfalse)=Aωα (Fabry et al., ; Desprat et al., ) both at the whole cell scale and at the intracellular local scale, independently of the experimental technique or the cell type (Balland et al., ). Still, one study in a hepatocyte carcinoma cell line (Hep G2) shows that a power‐law cannot describe well the solid behaviour of the cell at low frequencies (Wang et al., ). Several theoretical models of cell rheology predict that G(ω) should follow a power‐law.…”
Section: Why May Cancer Cells Be Softer Than Normal Cells?mentioning
confidence: 99%
“…Consequently, the model leads to more extensive parameter sets to characterize and analyze the biomechanical properties of cell components. There have been only a few studies based on the GM model for characterizing cell viscoelastic properties by AFM nano-indentation [21,22], however the comparison of the biomechanical characterization of the sub-cellular structures among normal and cancerous cells has been left untouched.…”
Section: Introductionmentioning
confidence: 99%