A Gene Probe for the Patulin Metabolic Pathway with Potential for Use in Patulin and Novel Disease Control

Paterson, R. R. M.; Archer, S. A.; Kozakiewicz, Z.; Lea, Arden O.; Locke, T.; O'Grady, E.P

doi:10.1080/09583150050115098

Cited by 46 publications

(17 citation statements)

References 4 publications

(2 reference statements)

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“…A detection system for patulin-producing fungi based on the isoepoxydon dehydrogenase (IDH) gene was described by Paterson et al (43). As expected, all patulin-producing fungi tested showed a PCR product, indicating the presence of the IDH gene of the patulin biosynthetic pathway (Fig.…”

Section: Patulin-producing Penicillium Speciesmentioning

confidence: 99%

“…5) and trichothecens ( Fig. 6) (43,49). Many genes involved in the biosynthesis of these mycotoxins have been identified and their DNA sequences have been published.…”

Section: Development Of a Pcr Assaymentioning

confidence: 99%

“…The trichodiene synthase encoding gene tri5, a yet non-identified protein encoding gene tri7, and the regulatory gene tri6 have been the targets for detecting trichotheceneproducing Fusaria (6,42). The target sequence for the detection of patulin-producing Penicillum strains is within the isoepoxydon dehydrogenase encoding gene IDH (43).…”

Section: Development Of a Pcr Assaymentioning

confidence: 99%

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The application of PCR in the detection of mycotoxigenic fungi in foods

Konietzny¹,

Greiner²

2003

Braz. J. Microbiol.

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It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene-as well as fumonisin-producing Fusaria strains have been described. The usefulness of the PCR methods developed so far to monitor quality and safety in the food an feed industry was already demonstrated. Thus, PCR may be applied to the screening of agricultural commodities for the absence of mycotoxin producers prior to or even after processing. Negative results in this assay indicate that a sample should be virtually free of mycotoxins. Only the positive samples left must be analyzed for the presence of mycotoxins using physicochemical standard methods. This review does not only summarize the so far developed qualitative and quantitative PCR assays for the detection of mycotoxigenic fungi in agricultural commodities, foods and animal feeds, but describes also strategies to develop new specific PCR assays for such a detection.

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Section: Patulin-producing Penicillium Speciesmentioning

confidence: 99%

“…5) and trichothecens ( Fig. 6) (43,49). Many genes involved in the biosynthesis of these mycotoxins have been identified and their DNA sequences have been published.…”

Section: Development Of a Pcr Assaymentioning

confidence: 99%

Section: Development Of a Pcr Assaymentioning

confidence: 99%

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The application of PCR in the detection of mycotoxigenic fungi in foods

Konietzny¹,

Greiner²

2003

Braz. J. Microbiol.

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“…The ability by P. expansum to produce patulin is particularly considered in apples, a few stone fruits and grapes, and the finding of this mycotoxin in symptomless fruits has raised concerns for a possible intake deriving from latent strains [20,21]. To this regard no circumstantial evidence has resulted so far, despite a reported endophytic ability of this species (Table 1).…”

Section: Phytopathological Implicationsmentioning

confidence: 89%

Endophytism of Penicillium Species in Woody Plants

Nicoletti¹,

Fiorentino²,

Scognamiglio³

2014

TOMYCJ

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Abstract:The genus Penicillium is ubiquitous and its species are commonly recovered from every kind of substrate and environmental conditions. Therefore, not surprisingly, Penicillium strains are commonly reported in investigations dealing with fungal endophytic assemblages of plants in both natural and anthropic contexts. As such they are implicated in more or less effective relationships with the host, and involved in applicative issues concerning plant protection and the production of bioactive compounds to be exploited as pharmaceuticals. The available data on occurrence, biocenotic role, production and bioactivities of secondary metabolites of Penicillium strains which have been isolated as endophytes from woody plants are reviewed in the present paper in the aim to introduce the state of the art to researchers involved in this particular field.

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“…Primers IDH2F, IDH1R and IDH2R were designed to amplify an approximately 600 bp region. Primer pair IDH3F and IDH3R had previously been used to compare isolates from apple orchards in the United Kingdom (Paterson et al 2000(Paterson et al , 2003.…”

Section: Nucleotide Sequencingmentioning

confidence: 99%

The isoepoxydon dehydrogenase gene of the patulin metabolic pathway differs for Penicillium griseofulvum and Penicillium expansum

Dombrink-Kurtzman

2005

Antonie Van Leeuwenhoek

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Purified DNA from isolates of Penicillium griseofulvum and P. expansum was used as a template to amplify a 600-bp fragment of the isoepoxydon dehydrogenase (idh) gene of the patulin biosynthetic pathway. Primer pairs designed from the P. griseofulvum gene to amplify specific regions of the idh gene yielded similar-sized bands for all strains. Asymmetrical amplification produced DNA products for sequencing and DNA sequences were translated to produce the corresponding amino acid sequences. After removal of two introns present in the region sequenced, amino acid sequences were compared. There were 12 amino acid differences between P. expansum and P. griseofulvum in the coding region. The differences correlated with the amount of patulin previously produced in culture, with strains of P. griseofulvum producing the greatest amounts of patulin.

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A Gene Probe for the Patulin Metabolic Pathway with Potential for Use in Patulin and Novel Disease Control

Cited by 46 publications

References 4 publications

The application of PCR in the detection of mycotoxigenic fungi in foods

The application of PCR in the detection of mycotoxigenic fungi in foods

Endophytism of Penicillium Species in Woody Plants

The isoepoxydon dehydrogenase gene of the patulin metabolic pathway differs for Penicillium griseofulvum and Penicillium expansum

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