A Gene Encoding l -Methionine γ-Lyase Is Present in Enterobacteriaceae Family Genomes: Identification and Characterization of Citrobacter freundii l -Methionine γ-Lyase

Methionine gamma‐lyase: The unique reaction mechanism, physiological roles, and therapeutic applications against infectious diseases and cancers

“…Manukhov et al reported that the C. freundii has a MGL gene while its vestigial gene was found in the other bacteria in Enterobacteriaceae family, such as Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and C. rodentium (81). These bacteria appear to have acquired MGL by lateral gene transfer from a common ancestor, and the latter have lost the function of MGL.…”

Pre-steady-state Kinetic and Structural Analysis of Interaction of Methionine γ-Lyase from Citrobacter freundii with Inhibitors

“…The calculated rate constants of the formation of the (E⅐L-AA) 2 complex in the reactions with L-alanine and L-norvaline are much less than the rate constants of the isotopic exchange of C␣-and C␤-protons in the both amino acids (Table 2). Consequently, the external aldimine (E⅐L-AA) 2 cannot be an intermediate in the exchange reactions. It seems likely that in its structure (Fig.…”

“…The absorbance at 500 nm during interaction MGL with L-alanine and L-norvaline is slightly changed (data not shown) indicating that the quinonoid is a temporary intermediate and is not accumulated in the reaction. The simplest mechanism that can account for the kinetic data is presented in Scheme 4, and it includes the conversion of internal aldimine, absorbing at 420 nm, into complex (E⅐L-AA) 1 , which absorbs at 320 nm, and slow conversion of this complex into the external aldimine (E⅐L-AA) 2 , which absorbs at 420 nm. The calculated rate constants of the formation of the (E⅐L-AA) 2 complex in the reactions with L-alanine and L-norvaline are much less than the rate constants of the isotopic exchange of C␣-and C␤-protons in the both amino acids (Table 2).…”

“…Growing the cells and purification of the enzyme were carried out as described previously (2). Protein concentrations were determined by the method of Lowry et al (22), using bovine serum albumin as a standard.…”

“…Methionine ␥-lyase (EC 4.4.1.11, MGL), 3 a pyridoxal 5Ј-phosphate (PLP)-dependent enzyme, is found in many bacteria (1), including the Enterobacteriaceae family (Citrobacter freundii (2)) and in pathogenic bacteria (Bacteroides sp. (3), Aeromonas sp.…”