A gene cluster in Aspergillus nidulans with an internally located cis-acting regulatory region

Arst, Herbert N.; MacDonald, D

doi:10.1038/254026a0

Cited by 116 publications

(56 citation statements)

References 24 publications

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“…Of the creA gene thus identified, a number of mutant alleles have been selected which are recessive to wild-type and which typically display non-hierarchical heterogeneity of mutant phenotypes (Arst & Bailey, 1977 ;van der Veen e t al., 1994). The CREA protein was postulated to be a negatively acting regulatory protein directly involved in regulating gene expression (Arst & Bailey, 1977 ;Arst & MacDonald, 1975). This was confirmed by Dowzer & Kelly (1989 who cloned creA and found it to encode a 'zinc finger' DNA-binding protein of the Cys,His, type.…”

Section: Introductionmentioning

confidence: 62%

An extreme creA mutation in Aspergillus nidulans has severe effects on D-glucose utilization

Veen

Ruijter²,

Visser³

1995

Microbiology

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AspergWus nidulans wild-type and the extreme carbon catabolite derepressed mutant creAd-30 were characterized with respect to enzyme activities, metabolite Concentrations and POlyol p o l s all related to glYCOlYSiS, after growth on mglucose. In the creAd-30 strain the enzymes hexokinase and fructose-6-phosphate reductase showed a two-and threefold increase in activity, respectively, whereas phosphofructokinase and pyruvate kinase activity decreased two-and threefold, respectively, in comparison with the wild-type strain. The most notable changes in metabolite concentrations were that fructose 2,C-bisphosphate and fructose 1,6=bisphosphate showed a 2.5-fold increase, whereas both pyruvate and citrate decreased in the creAd-30. Striking differences were found for the polyol concentrations measured for the two strains tested. lntracellular glycerol and arabitol concentrations were 10-fold higher and erythritol fivefold higher in creAd-30, whereas intracellular trehalose and mannitol were both decreased. The total internal polyol concentration appears to be constant at -700 pmol (g dry wt)? All polyols were also detected in high amounts in the culture filtrate of the creAd-30 mutant strain but no extracellular trehalose was found. The overall production of polyols in this strain was therefore much higher than in the wild-type. The high level of polyols produced and the changes in metabolite concentrations in the creAd-30 strain suggest that the differences in enzyme activities result in an altered flow through glycolysis leading to a more rapid formation of polyols which are subsequently secreted.

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Section: Introductionmentioning

confidence: 62%

An extreme creA mutation in Aspergillus nidulans has severe effects on D-glucose utilization

Veen

Ruijter²,

Visser³

1995

Microbiology

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“…Mycelia were transferred (see the Experimental procedures) to minimal media lacking proline and RNA samples were obtained from aliquots harvested after 30 min (lane 30Ј), 1 h (lane 1 h) and 2 h (lane 2 h) of incubation. the prnA gene (Arst and MacDonald, 1975;Jones et al, 1981;Sharma and Arst, 1985), prnB expression can be regulated by three different mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…The prnA gene codes for a proline-responsive transcriptional activator, prnX is a proline-inducible gene of unknown function, prnD codes for proline oxidase, prnB for the proline transporter and prnC for ⌬ 1 -pyrroline-5-carboxylate dehydrogenase. prnA, prnX and prnD are transcribed divergently from prnB and prnC and thus there is a region between the prnD and prnB genes which may involve cisacting elements affecting the transcription of both genes (Arst and MacDonald, 1975;Arst et al, 1980;Sharma and Arst, 1985;Sophianopoulou and Scazzocchio, 1989;Gavrias et al, 1994;Sophianopoulou et al, 1993;Cubero and Scazzocchio, 1994).…”

Section: Introductionmentioning

confidence: 99%

The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation

Tazebay

Sophianopoulou

Scazzocchio

et al. 1997

Molecular Microbiology

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SummaryIn Aspergillus nidulans a highly specific L-proline transporter is encoded by the prnB gene which is tightly linked to all other genes involved in proline catabolism. In mycelia, the expression of the prn structural genes is finely co-regulated in response to proline induction and nitrogen/carbon catabolite repression. In this study we establish that prnB expression is also activated during germination of conidiospores. This activation persists until the development of 6 h-old mycelia and it is independent of proline induction mediated by the pathway-specific prnA gene product. We then show that, in mycelia, prnB transcription is activated in response to proline or histidine starvation. This process has two components: a prnA-dependent and a prnA-independent component. A cis-acting element that conforms to the consensus target of the GCN4/CPC1 transcriptional activators mediating amino acid biosynthesis activation in other fungi is involved in the activation of prnB transcription in response to amino acid starvation. We also show that the stimulation of prnB expression in germinating conidiospores is not due exclusively to transient internal amino acid starvation occurring during the transition from conidiospore to mycelium. This is the first report that an amino acid transporter gene is upregulated during development and in response to amino acid starvation and specific amino acid induction.

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“…3B and 4B). The catabolism of proline requires activities encoded by the prn gene cluster (5). A deletion of the prn cluster resulted in a loss of proline but not acetate induction, showing that proline catabolism is required for induction (Fig.…”

Section: Resultsmentioning

confidence: 99%

Regulation of theacuFGene, Encoding Phosphoenolpyruvate Carboxykinase in the Filamentous FungusAspergillus nidulans

2002

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Phosphoenolpyruvate carboxykinase (PEPCK) is a key enzyme required for gluconeogenesis when microorganisms grow on carbon sources metabolized via the tricarboxylic acid (TCA) cycle. Aspergillus nidulans acuF mutants isolated by their inability to use acetate as a carbon source specifically lack PEPCK. The acuF gene has been cloned and shown to encode a protein with high similarity to PEPCK from bacteria, plants, and fungi. The regulation of acuF expression has been studied by Northern blotting and by the construction of lacZ fusion reporters. Induction by acetate is abolished in mutants unable to metabolize acetate via the TCA cycle, and induction by amino acids metabolized via 2-oxoglutarate is lost in mutants unable to form 2-oxoglutarate. Induction by acetate and proline is not additive, consistent with a single mechanism of induction. Malate and succinate result in induction, and it is proposed that PEPCK is controlled by a novel mechanism of induction by a TCA cycle intermediate or derivative, thereby allowing gluconeogenesis to occur during growth on any carbon source metabolized via the TCA cycle. It has been shown that the facB gene, which mediates acetate induction of enzymes specifically required for acetate utilization, is not directly involved in PEPCK induction. This is in contrast to Saccharomyces cerevisiae, where Cat8p and Sip4p, homologs of FacB, regulate PEPCK as well as the expression of other genes necessary for growth on nonfermentable carbon sources in response to the carbon source present. This difference in the control of gluconeogenesis reflects the ability of A. nidulans and other filamentous fungi to use a wide variety of carbon sources in comparison with S. cerevisiae. The acuF gene was also found to be subject to activation by the CCAAT binding protein AnCF, a protein homologous to the S. cerevisiae Hap complex and the mammalian NFY complex.

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A gene cluster in Aspergillus nidulans with an internally located cis-acting regulatory region

Cited by 116 publications

References 24 publications

An extreme creA mutation in Aspergillus nidulans has severe effects on D-glucose utilization

An extreme creA mutation in Aspergillus nidulans has severe effects on D-glucose utilization

The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation

Regulation of theacuFGene, Encoding Phosphoenolpyruvate Carboxykinase in the Filamentous FungusAspergillus nidulans

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