A gelatinized lipopeptide diet effectively modulates immune response, disease resistance and gut microbiome in Penaeus vannamei challenged with Vibrio parahaemolyticus

Prathiviraj, R.; Rajeev, Riya; Fernandes, Henrietta; Kumarasamy, Rathna; Lipton, Anuj Nishanth; Selvin, Joseph; Kiran, George Seghal

doi:10.1016/j.fsi.2021.02.018

Cited by 21 publications

(8 citation statements)

References 48 publications

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“…The best hits were classified based on the percentage of identity and sequence query coverage. The rarefaction curve plot was used to annotate the species richness among the samples based on Rarefaction Curve Analysis [47][48][49][50]. A genus-level relative abundance of an operational taxonomic unit (OTUs) was computed and compared for a better understanding of the composition of a microbial community among the selected samples.…”

Section: Samplesmentioning

confidence: 99%

Microbiome Changes of Endemic Lake Baikal Sponges during Bleaching Syndrome Development

et al. 2021

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The sponge (Porifera) microbiome is an indicator of both natural and anthropogenic stressors. Studying Baikal sponge microbial communities could help reveal if there is a connection between bacterial symbionts and a mass sponge bleaching event that was recently detected; 16S rRNA sequencing was performed among healthy and diseased freshwater sponges of Lubomirskia baikalensis and Baikalospongia intermedia, which were collected from Lake Baikal, Russia. A phylum-based taxonomic classification showed that Chlorophyta, Acidobacteria, Bacteroidetes, Actinobacteria and Cyanobacteria were most abundant across samples. When comparing healthy and diseased L. baikalensis samples, large variations in microbial composition were found at the phylum level. Comparative analyses, which were performed for the first time for B. intermedia, showed a decrease in Chlorophyta (unicellular green algae) and an increase in Bacteroidetes and Cyanobacteria in diseased specimens. At the genus level, the Opitutus (Verrucomicrobia), Planctomyces, and Nitrospira content increased in all diseased sponges, which reflected a general tendency toward an increase in Cyanobacteria in diseased sponges. Comparative analysis of the diseased and healthy sponge metagenomes showed that diseased sponges underwent various nonspecific changes in bacterial composition. The bacterial community composition is probably influenced by sponge type and degree of disease affection.

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Section: Samplesmentioning

confidence: 99%

Microbiome Changes of Endemic Lake Baikal Sponges during Bleaching Syndrome Development

et al. 2021

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“…After phosphorylation, Spo0A regulates biosynthesis of many secondary metabolites (Rahman et al, 2006;Sun et al, 2021). For example, Klausmann et al (2021) reported that the null-mutant of spo0A could reduce surfactin production in B. subtilis. Consistently, deletion of spo0A led to a very significant reduction of antifungal activity in WH1.…”

Section: Discussionmentioning

confidence: 99%

“…Many Bacillus species such as B. amyloliquefaciens , B. subtilis , B. velezensis , etc, are well-known bacteria for producing antifungal lipopeptides such as iturin and fengycin ( Kaspar et al, 2019 ), which are green and broad-spectrum fungicides with potential use in developing biological pesticides ( Zhao et al, 2017b ), food preservatives ( Kourmentza et al, 2021 ; Gu et al, 2022 ), antifungal and antitumor drugs ( Lin et al, 2020 ; Wan et al, 2021 ), feed additives ( Zhao et al, 2017a ; Prathiviraj et al, 2021 ), etc. Iturin, a cyclic lipoheptapeptide that is linked to a fatty acid chain with 14–17 carbon number, has a broad-spectrum inhibitory effect on many plant pathogenic fungi.…”

Section: Introductionmentioning

confidence: 99%

Systemically engineering Bacillus amyloliquefaciens for increasing its antifungal activity and green antifungal lipopeptides production

Wang

Wang²,

Zhao

et al. 2022

Front. Bioeng. Biotechnol.

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The biosynthesis of antifungal lipopeptides iturin and fengycin has attracted broad interest; however, there is a bottleneck in its low yield in wild strains. Because the key metabolic mechanisms in the lipopeptides synthesis pathway remain unclear, genetic engineering approaches are all ending up with a single or a few gene modifications. The aim of this study is to develop a systematic engineering approach to improve the antifungal activity and biosynthesis of iturin and fengycin in Bacillus amyloliquefaciens. First, blocking the carbon overflow metabolic pathway to increase precursor supply of the branched-chain amino acids by knockout of bdh, disrupting sporulation to extend the stage for producing antifungal lipopeptides by deletion of kinA, blocking of siderophore synthesis to enhance the availability of amino acids and fatty acids by deletion of dhbF, and increasing Spo0A∼P by deletion of rapA, could improve the antifungal activity by 24%, 10%, 13% and 18%, respectively. Second, the double knockout strain ΔbdhΔkinA, triple knockout strain ΔbdhΔkinAΔdhbF and quadruple knockout strain ΔkinAΔbdhΔdhbFΔrapA could improve the antifungal activity by 38%, 44% and 53%, respectively. Finally, overexpression of sfp in ΔkinAΔbdhΔdhbFΔrapA further increased the antifungal activity by 65%. After purifying iturin and fengycin as standards for quantitative analysis of lipopeptides, we found the iturin titer was 17.0 mg/L in the final engineered strain, which was 3.2-fold of the original strain. After fermentation optimization, the titer of iturin and fengycin reached 31.1 mg/L and 175.3 mg/L in flask, and 123.5 mg/L and 1200.8 mg/L in bioreactor. Compared to the original strain, the iturin and fengycin titer in bioreactor increased by 22.8-fold and 15.9-fold in the final engineered strain, respectively. This study may pave the way for the commercial production of green antifungal lipopeptides, and is also favorable for understanding the regulatory and biosynthetic mechanism of iturin and fengycin.

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“…It was also been reported that Bradyrhizobium obtained the greatest number of sequences in intestinal microbiota from adult pirarucu ( Arapaima gigas ) ( Pereira et al, 2017 ). Among the research of diet-affected changes on gut microbiome of shrimp, Streptomyces was one of the most abundant genera on diet-fed group ( Prathiviraj et al, 2021 ). Several genera mentioned in this study could also been served as the probiotics in sustainable aquaculture, for example, Streptomyces and Bacillus were reported to be good candidates to protect fish and shrimp from pathogens ( Tan et al, 2016 ), or improve feed utilization and disease resistance ( Kuebutornye et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Exploring the Dynamic of Bacterial Communities in Manila Clam (Ruditapes philippinarum) During Refrigerated Storage

2022

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Microorganism contamination is one of the most important factors affecting the spoilage and food safety of Manila clams. This study aimed to gain insights into bacterial composition and the dynamic change of bacterial communities on retailed Manila clam during refrigerated storage within the edible period. High-throughput sequencing was conducted to monitor the bacterial population with the prolongation of storage time of Day 0, Day 1, and Day 3. Result demonstrated that phyla of Proteobacteria, Actinobacteriota, Acidobacteriota, and Chloroflexi composed the majority of bacterial communities during the whole observation process. Furthermore, the increase of Proteobacteria showed a positive correlation with the storage time, whereas Acidobacteriota and Chloroflexi continued to decline in storage. For genus annotation, none of genus obtained dominant population in storage. From Day 0 to Day 1, the genera of Streptomyces, Bradyrhizobium, and Mycobacterium significantly increased; meanwhile, 12 genera significantly decreased. Compared with samples at Day 0, a total of 15 genera significantly decreased with the reduced proportion ranging from 0.50 to 4.40% at Day 3. At the end of the storage, the genus Crossiella became the most redundant population. Both the richness and diversity decreased at the start of storage at Day 1, and then slightly increased at Day 3 was observed. Based on the result in this study, strategy targeting the increased bacteria could be tested to improve the consumption quality and safety of refrigerated clam.

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A gelatinized lipopeptide diet effectively modulates immune response, disease resistance and gut microbiome in Penaeus vannamei challenged with Vibrio parahaemolyticus

Cited by 21 publications

References 48 publications

Microbiome Changes of Endemic Lake Baikal Sponges during Bleaching Syndrome Development

Microbiome Changes of Endemic Lake Baikal Sponges during Bleaching Syndrome Development

Systemically engineering Bacillus amyloliquefaciens for increasing its antifungal activity and green antifungal lipopeptides production

Exploring the Dynamic of Bacterial Communities in Manila Clam (Ruditapes philippinarum) During Refrigerated Storage

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