A Ge.F.I. – ISFG European collaborative study on DNA identification of Cannabis sativa samples using a 13-locus multiplex STR method

Nunzio, Michele Di; Agostini, Vincenzo; Alessandrini, Federica; Barrot-Feixat, Carme; Berti, Andrea; Bini, Carla; Bottinelli, Michel; Carnevali, E.; Corradini, Beatrice; Fabbri, M.; Fattorini, Paolo; Garofano, Paolo; Gino, Sarah; Mameli, A; Marinò, Alberto; Previderé, Carlo; Robino, Carlo; Romano, C.; Tozzo, Pamela; Verzeletti, Andrea; Buscemi, Loredana; Gangitano, David; Nunzio, Ciro Di

doi:10.1016/j.forsciint.2021.111053

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Accuracy in animal forensic genetic testing was explored with interlaboratory assessments performed in 2016 and 2018 [ 629 ]. A collaborative exercise conducted in 2020 and 2021 by the ISFG Italian Speaking Working Group examined performance across 21 laboratories with a 13-locus STR marker test for Cannabis sativa [ 630 ]. A molecular approach was explored to distinguish drug-type versus fiber-type hemp varieties [ 631 ].…”

Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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“…Many illegal drugs derived from plants, such as Cannabis sativa L. [19][20][21][22][23][24] , Papaver somniferum 25,26 , and Panaeolus cambodginiensis 27 , are often problematic. Moreover, some toxic plants show strong morphological similarities to edible plants or herbs, and poisoning is frequently caused by accidental ingestion [28][29][30][31][32][33] . If all toxic plants can be analyzed in detail, we can determine the origin of the sample.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Camellia cultivars using iD-NA analysis

Kikkawa

Sato

Matsuo

et al. 2023

Preprint

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Recently, many new cultivars are taken abroad illegally, which is now considered an international issue. Botanical evidence found at a crime scene provides valuable information about the origin of the sample. However, botanical resources for forensic evidence remain underutilized because molecular markers, such as microsatellites, are not available without a limited set of species. Multiplexed ISSR genotyping by sequencing (MIG-seq) has been used to determine a number of genome-wide genetic markers and can be applied for every plant, including species that lack genetic information. Camellia cultivars are popular worldwide and are often planted in many gardens and bred to make new cultivars. In this study, we aimed to analyze Camellia cultivars/species through MIG-seq. Through this, MIG-seq could discriminate similar samples, such as bud mutations and closely related samples which could not be distinguished based on morphological features. This discrimination was consistent with that of a previous study which classified cultivars by short tandem repeat (STR) markers, indicating that MIG-seq has the same or higher discrimination ability as STR markers. Furthermore, based on our results, unknown phylogenetic relationships were observed. Since MIG-seq can be applied to unlimited species and low-quality DNA, it may be useful for various scientific fields.

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“…Many illegal drugs derived from plants such as Cannabis sativa L. 6 – 11 , Papaver somniferum 12 , 13 , and Panaeolus cambodginiensis 14 are often problematic. Moreover, some toxic plants show strong morphological similarities to edible plants or herbs, and poisoning is frequently caused by accidental ingestion 15 – 20 . If all toxic plants can be analyzed in detail, we can determine the origin of the sample.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Camellia cultivars using iD-NA analysis

Kikkawa,

Sato,

Matsuo

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Recently, many new cultivars have been taken abroad illegally, which is now considered an international issue. Botanical evidence found at a crime scene provides valuable information about the origin of the sample. However, botanical resources for forensic evidence remain underutilized because molecular markers, such as microsatellites, are not available without a limited set of species. Multiplexed intersimple sequence repeat (ISSR) genotyping by sequencing (MIG-seq) and its analysis method, identification of not applicable (iD-NA), have been used to determine several genome-wide genetic markers, making them applicable to all plant species, including those with limited available genetic information. Camellia cultivars are popular worldwide and are often planted in many gardens and bred to make new cultivars. In this study, we aimed to analyze Camellia cultivars/species through MIG-seq. MIG-seq could discriminate similar samples, such as bud mutants and closely related samples that could not be distinguished based on morphological features. This discrimination was consistent with that of a previous study that classified cultivars based on short tandem repeat (STR) markers, indicating that MIG-seq has the same or higher discrimination ability as STR markers. Furthermore, we observed unknown phylogenetic relationships. Because MIG-seq can be applied to unlimited species and low-quality DNA, it may be useful in various scientific fields.

show abstract

A Ge.F.I. – ISFG European collaborative study on DNA identification of Cannabis sativa samples using a 13-locus multiplex STR method

Cited by 6 publications

References 21 publications

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Discrimination of Camellia cultivars using iD-NA analysis

Discrimination of Camellia cultivars using iD-NA analysis

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