A gas chromatography full scan high resolution Orbitrap mass spectrometry method for separation and characterization of 3-hydroxymethyl pyridine ester of fatty acids at low levels

Merlier, Franck; Imatoukene, Nabila; Octave, Stéphane; Nicaud, Jean‐Marc; Thomasset, Brigitte

doi:10.1016/j.chroma.2018.09.010

Cited by 12 publications

(6 citation statements)

References 33 publications

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“…Fatty acid profiles were separated and analyzed by a gas chromatography with flame ionization detection (GC-FID 2010 Plus Shimadzu) equipped with a BPX 70 capillary column (SGE, 30 m × 0.25 mm, 0.25 µm) as described in Merlier et al 86 . The fatty acids were identified by comparison of the retention times of a standard solution of 37 fatty acid methyl esters (Sigma; 47885-U Supelco) in GC-FID and confirmed by high accuracy mass of molecular ions and their fragments after injection into a GC-MS (Q-Exactive™, Thermo) 86 . The composition of fatty acids was expressed as a relative percentage of their peak areas with respect to the total peak area of all the fatty acids.…”

Section: Fames Analysismentioning

confidence: 99%

“…From fat bodies of different queen stages, fatty acid profiles were separated and analyzed by a gas chromatography with flame ionization detection (GC-FID 2010 Plus Shimadzu) equipped with a BPX 70 capillary column (SGE, 30 m × 0.25 mm, 0.25 μm) as described in Merlier et al 90…”

Section: Fames Analysismentioning

confidence: 99%

“…The fatty acids were identified by comparison of the retention times of a standard solution of 37 fatty acid methyl esters (Sigma; 47885-U Supelco) in GC-FID and confirmed by high accuracy mass of molecular ions and their fragments after injection into a GC-MS (Q-Exactive™, Thermo)90 . The composition of fatty acids was expressed as a relative percentage of their peak areas with respect to the total peak area of all the fatty acids.…”

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confidence: 99%

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Lifespan prolonging mechanisms and insulin upregulation without fat accumulation in long-lived reproductives of a higher termite

Séité

Sillam‐Dussès

et al. 2021

Preprint

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Eusocial termite queens achieve nearly maximal fertility throughout their extremely long life without apparent signs of aging. Termites represent, therefore, an ideal model for aging research. To investigate the molecular mechanisms underlying their long reproductive life, we carried out transcriptomic, lipidomic and metabolomic analyses on fat bodies of sterile short lived workers, long-lived kings and five stages spanning twenty years of adult queen maturation. In mature reproductives, genes supporting a robust mitochondrial functioning or associated with genome stability were upregulated. In most organisms, insulin signaling increases fertility but decreases lifespan, often accompanied by harmful lipid signatures. Our findings suggest that an upregulation of insulin-like peptide (Ilp9) in the fat body of termite queens is accompanied by a specific lipid metabolism, limiting fat storage, thus sustaining both high fertility and maintaining extreme lifespan. Our results highlight potential molecular targets for research into aging-related metabolic diseases linked to the accumulation of excess fat.

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Section: Fames Analysismentioning

confidence: 99%

Section: Fames Analysismentioning

confidence: 99%

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confidence: 99%

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Lifespan prolonging mechanisms and insulin upregulation without fat accumulation in long-lived reproductives of a higher termite

Séité

Sillam‐Dussès

et al. 2021

Preprint

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“…These FAs exhibit longterm resistance to oxidation and low temperature fluidity. Mass determination and MS spectra confirmed that these CFAs contain C17:0ca, and C19:0ca (Merlier, Imatoukene, Octave, Nicaud, & Thomasset, 2018) and correspond to the transformation of the C16:1 and C18:1 FA into C17:0ca and C19:0ca, respectively, through CFAs activity. The increase in palmitic acid after degradation is similar to the observation made by that some fatty acids increased in amounts compared to the initial amount before degradation.…”

Section: Resultsmentioning

confidence: 78%

Monitoring degradation of restaurant wastewater byLysinibacillus sphaericusC3-41

Aderiye

Iteke

Akinyeye

et al. 2021

Arab Journal of Basic and Applied Sciences

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This study investigates the biodegradation abilities of Lysinibacillus sphaericus C3-41 in restaurant wastewater. The extent of biodegradation was monitored using Fourier Transform Infrared Spectroscopy (FTIR) and Fatty Acid Methyl Ester (FAME) analysis. There was significant reduction in mineral content (p < 0.05) of the wastewater after 6 days incubation while the highest growth (1.4 OD 600 ) and lipase production (50mMol/min) was observed on the 5 th day. About 2.5% (v/v) oil concentration and pH 8 were found to be optimal conditions for growth and lipase production, while a temperature of 30 C, and 1% ammonium phosphate and glucose were detected as optimum nitrogen and carbon sources respectively. FAME analyses revealed the presence of some fatty acids such as palmitic acid (11.56 ppm), caprylic (11.22 ppm), caproic (11.19 ppm), lauric acid (9.95 ppm), undecanoic (4.77 ppm) and cyclopropane fatty acid (CFA, 4.34 ppm) in the fresh domestic wastewater. After biodegradation, there was an increase in the concentration of capric (0.39 ppm), lauric (0.33 ppm), palmitoleic (0.78 ppm), trans13 octadecanoic (1.00 ppm) and cyclopropanoic (2.53 ppm) in L. sphaericus C3-41. Also, reduction in concentration of some fatty acids components in the wastewater probably eventually resulted in their bioaccumulation in the bacteria cell. FTIR spectra revealed the changes in functional groups qualitatively and quantitatively with evidence of wastewater biodegradation by L. sphaericus C3-41. The prominent -NH stretching bonds at 3740 cm À1 from the primary and secondary amines completely disappeared after the 6 th day, indicating chemical reduction of -NH bonds leading to liberation of NH 3(g) . This study thus confirms the bioremediation potentials of L. sphaericus C3-41 for domestic and restaurant wastewater.

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“…These two new FA appearing when EcCFAs is expressed have been analyzed by GC‐MS and have been formerly characterized (Figure S1). Mass determination and MS spectra confirmed that these FA are indeed CFA, C17:0ca, and C19:0ca (Merlier, Imatoukene, Octave, Nicaud, & Thomasset, ) and correspond to the transformation of the C16:1 and C18:1 FA into C17:0ca and C19:0ca, respectively, through CFAs activity. Our data are in agreement with those mentioned in the literature when EcCFAs is overexpressed in Arabidopsis thaliana , Nicotiana tabaccum , or Cyanobacterium synecchocytis (Machida et al, ; Schmid, ; Shanklin, Yu, & Prakash, ).…”

Section: Resultsmentioning

confidence: 90%

Optimization of cyclopropane fatty acids production in Yarrowia lipolytica

Czerwiec

Idrissitaghki

Imatoukene

et al. 2019

Yeast

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Cyclopropane fatty acids, which can be simply converted to methylated fatty acids, are good unusual fatty acid candidates for long‐term resistance to oxidization and low‐temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amounts in plants or bacteria. In order to develop a process for large‐scale biolipid production, we expressed 10 cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amounts of lipids. The Escherichia coli cyclopropane fatty acid synthase expression in Y. lipolytica allows the production of two classes of cyclopropane fatty acids, a C17:0 cyclopropanated form and a C19:0 cyclopropanated form, whereas others produce only the C17:0 form. Expression optimization and fed‐batch fermentation set‐up enable us to reach a specific productivity of 0.032 g·L−1·hr−1 with a genetically modified strain containing cyclopropane fatty acid up to 45% of the total lipid content corresponding to a titre of 2.3 ± 0.2 g/L and a yield of 56.2 ± 4.4 mg/g.

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A gas chromatography full scan high resolution Orbitrap mass spectrometry method for separation and characterization of 3-hydroxymethyl pyridine ester of fatty acids at low levels

Cited by 12 publications

References 33 publications

Lifespan prolonging mechanisms and insulin upregulation without fat accumulation in long-lived reproductives of a higher termite

Lifespan prolonging mechanisms and insulin upregulation without fat accumulation in long-lived reproductives of a higher termite

Monitoring degradation of restaurant wastewater byLysinibacillus sphaericusC3-41

Optimization of cyclopropane fatty acids production in Yarrowia lipolytica

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