A ganglioside antigen on the rat pancreatic B cell surface identified by monoclonal antibody R2D6.

Alejandro, Rodolfo; Shienvold, Frances L.; Hajek, S A; Pierce, Mark; Paul, Rajani Kumar; Mintz, Daniel H.

doi:10.1172/jci111409

Cited by 36 publications

(1 citation statement)

References 37 publications

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“…Hanks' balanced salt solution saturated with a mixture of 95% O 2 and 5% CO 2 was used during the isolation procedure. Dispersed cells were prepared from isolated islets by incubation for 5-10 min at 30˚C in a Ca 2+ -free Hepes-NaOH buffer containing neutral proteinase (EC 3.4.24.4, 0.48 mg/ml) from Bacillus polymyxa (Dispase II; Boehringer Mannheim, Germany) and 2 populations of B and non-B cells, respectively, were obtained by immunomagnetic purification, as described elsewhere (17) using R2D6 monoclonal antibody directed against plasma membranes of rat pancreatic B cells (18).…”

Section: Methodsmentioning

confidence: 99%

Fructokinase activity in rat liver, ileum, parotid gland, pancreas, pancreatic islet, B and non-B islet cell homogenates

Giroix

Jijakli²,

Courtois³

et al. 2006

Int J Mol Med

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Abstract. The presence of fructokinase (ketohexokinase) in rat pancreatic islet homogenates was previously documented. However, no information was so far available on the activity of this enzyme in islets relative to that in other tissues and on the respective contribution of insulin-producing B cells and non-B islet cells. The present study provides such an information. The activity of fructokinase, as assessed by the phosphorylation of 1.0 mM D-fructose, was compared to that of hexokinase isoenzyme(s), as measured in the presence of 1.0 mM Dglucose, and further characterized by its heat-resistance, K + dependency and resistance to the inhibitory action of D-mannoheptulose. As judged from the results obtained in heated homogenates, the activity of fructokinase, expressed relative to protein content (nmol/min per mg protein) was highest in liver (21.5±2.5; n=11) and lowest in parotid gland (0.16±0.09; n=3), with in-between values in ileum (2.45±0.53; n=3), pancreas (0.82±0.11; n=11) and pancreatic islets (0.46±0.07; n=6). The paired ratio between fructokinase and hexokinase isoenzyme activity was also highest in liver (548±45%; n=8) and lowest in parotid gland (0.93±0.52%; n=3). Such a ratio was not significantly different in pancreas, islets and purified B or non-B islet cells, with an overall mean value of 2.57±0.46% (n=12). The present findings thus unambiguously document the presence of fructokinase activity in all cell types under consideration, except possibly parotid cells, with the following hierarchy: liver > ileum > pancreas. Relative to paired hexokinase activity, no obvious difference was found for fructokinase activity in B versus non-B islet cells.

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Section: Methodsmentioning

confidence: 99%

Fructokinase activity in rat liver, ileum, parotid gland, pancreas, pancreatic islet, B and non-B islet cell homogenates

Giroix

Jijakli²,

Courtois³

et al. 2006

Int J Mol Med

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Pancreatic fate of a ¹²⁵I‐labelled mouse monoclonal antibody directed against pancreatic B‐cell surface ganglioside(s) in control and diabetic rats

Ladrière¹,

Malaisse‐Lagae²,

Alejandro

et al. 2001

Cell Biochemistry & Function

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The possible use of a mouse monoclonal antibody directed against rat pancreatic B-cell surface ganglioside(s) and labelled with radioactive iodine for selective imaging of the endocrine pancreas by a non-invasive procedure was investigated by following its pancreatic fate in experiments conducted either in vitro by incubation of rat isolated pancreatic islets, acinar tissue and pancreatic pieces or in vivo after intravenous injection of the (125)I-labelled antibodies ([(125)I]gamma-G). Although the binding of [(125)I]gamma-G per microg protein was about one order of magnitude higher in isolated islets than in acinar tissue, no significant difference was detected when comparing pancreatic pieces or isolated islets from control animals and rats rendered diabetic by one or two prior administrations of streptozotocin (STZ rats). Likewise, except in one set of experiments, no significant difference was found between control animals and STZ rats, when measuring the radioactive content of the pancreatic gland, relative to that of plasma, 1-4 days after the intravenous injection of [(125)I]gamma-G. These findings indicate that under the present experimental conditions, the mouse monoclonal antibody labelled with radioactive iodine does not appear to be a promising tool for selective imaging of the endocrine pancreas, e.g. by single photon emission computerized tomography.

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Islet‐specific immune mechanisms

Lernmark

Shen

Bækkeskov

et al. 1987

Diabetes Metab. Rev.

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A ganglioside antigen on the rat pancreatic B cell surface identified by monoclonal antibody R2D6.

Cited by 36 publications

References 37 publications

Fructokinase activity in rat liver, ileum, parotid gland, pancreas, pancreatic islet, B and non-B islet cell homogenates

Fructokinase activity in rat liver, ileum, parotid gland, pancreas, pancreatic islet, B and non-B islet cell homogenates

Pancreatic fate of a ¹²⁵I‐labelled mouse monoclonal antibody directed against pancreatic B‐cell surface ganglioside(s) in control and diabetic rats

Islet‐specific immune mechanisms

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A ganglioside antigen on the rat pancreatic B cell surface identified by monoclonal antibody R2D6.

Cited by 36 publications

References 37 publications

Fructokinase activity in rat liver, ileum, parotid gland, pancreas, pancreatic islet, B and non-B islet cell homogenates

Fructokinase activity in rat liver, ileum, parotid gland, pancreas, pancreatic islet, B and non-B islet cell homogenates

Pancreatic fate of a 125I‐labelled mouse monoclonal antibody directed against pancreatic B‐cell surface ganglioside(s) in control and diabetic rats

Islet‐specific immune mechanisms

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Pancreatic fate of a ¹²⁵I‐labelled mouse monoclonal antibody directed against pancreatic B‐cell surface ganglioside(s) in control and diabetic rats