A galactosidase immunosorbent test for human immunoglobulin E

Weltman, Joel K.; Frackelton, A. Raymond; Szaro, Robert P.; Rotman, Boris

doi:10.1016/0091-6749(76)90123-8

Cited by 30 publications

(11 citation statements)

References 12 publications

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“…However only RIA has previously been sensitive enough for IgE measurements so the ELISA developed for IgE by Hoffmann (1973) and by Weltman et al (1976) could be of considerable practical value. Ferritin and alpha-2-haptoglobin have been assayed by ELISA but with lower sensitivity than with RIA (Miedema et al, 1972;Fuccaro-Decaens et al, 1974;Boenisch, 1976;) but Bartlett et al (1976) obtained good correlations between ELISA and existing methods in their studies on factor VIII.…”

Section: Serum Proteinsmentioning

confidence: 99%

Enzyme immunoassays with special reference to ELISA techniques.

Voller¹,

Bartlett²,

Bidwell³

1978

J Clin Pathol

629

257

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Section: Serum Proteinsmentioning

confidence: 99%

Enzyme immunoassays with special reference to ELISA techniques.

Voller¹,

Bartlett²,

Bidwell³

1978

J Clin Pathol

629

257

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“…Intolerance to aspirin was determined by chnical history (21). The galactosidase immunosorbent test (GIST) for specific IgE antibodies against aspirin and tartrazine was performed by adaptation of the method previously reported (28). Serum total IgE (RIST, Pharmacia) and total eosinophil count (TEC) were determined as described (5,7).…”

Section: Methodsmentioning

confidence: 99%

“…Intolerance to tartrazine was determined by double-blind crossover oral chaiienge with at least 0.22 mg of tartrazine, as previsouly reported (22). After 2 days in the dark at room temperature, the reaction mixture was decanted and the discs were washed exhaustively and sequentially with HjO, 6 M urea and E~buffer (28), until the wash solution possessed no detectable ASA or tartrazine as determined from fluorescence emission at 514 nm (yexcitation = 305 nm) and absorbance at 420 nm, respectively. Aliergy skin tests were perfomried by the scratch test method with a set of common pollen, mold and animal dander inhalent allergens.…”

Section: Methodsmentioning

confidence: 99%

An Analysis of the Role of IgE in Intolerance to Aspirin and Tartrazine

Weltman

Szaro

Settipane

1978

Allergy

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Total serum IgE and eosinophil count were determined for 30 patients with intolerance to aspirin. Total IgE levels in the aspirin intolerant patients were similar to those expected in a non-atopic population. In contrast, total eosinophil count (TEC) tended to be elevated in the aspirin intolerant group. Elevated TEC was observed both in bronchospastic (57%) and in urticarial (25%) aspirin intolerance. Specific anti-aspiryl and anti-tartrazyl antibodies of the IgE class were assayed by the galactosidase immunosorbent test (GIST). IgE anti-aspiryl antibodies were possibly detected in one patient, but did not correlate with clinical intolerance to aspirin. It is unlikely that the clinical symptoms and the eosinophilia of intolerance to aspirin and tartrazine are mediated by antibodies of the IgE class.

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“…Furthermore, a modifica tion of the ELISA, utilizing galactosidase as the enzyme instead of the alkaline phospha tase, has been employed for determination of ragweed-specific IgE antibodies and good agreement with RAST has been found in a small group of patients [14].…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients

Eriksson¹,

Ahlstedt²

1977

Int Arch Allergy Immunol

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An enzyme-linked immunosorbent assay (ELISA) employing allergen adsorbed onto the internal surface of a plastic tube, and alkaline phosphatase conjugated anti-IgE was used for the determination of specific IgE antibodies to various inhalant allergens in serum samples from 255 individuals with asthma and/or allergic rhinitis. A total of 541 analyses were carried out and the results were compared with those of provocation tests, skin tests and the RAST. It was found that negative ELISA values with high probability indicated nonallergy and very high ELISA values (class ≥ 5) indicated allergy. However, the proportion of positive ELISA values which did not correspond to clinical allergy was high, as were the number of intermediate, inconclusive values (class 1–2). Although significant correlations between the ELISA values and provocation test, skin test or the RAST were obtained with some allergens, it was concluded that our version of the ELISA has no advantages over currently used methods for allergy diagnosis.

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A galactosidase immunosorbent test for human immunoglobulin E

Cited by 30 publications

References 12 publications

Enzyme immunoassays with special reference to ELISA techniques.

Enzyme immunoassays with special reference to ELISA techniques.

An Analysis of the Role of IgE in Intolerance to Aspirin and Tartrazine

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients

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