A gain-of-function mutation of the MATE family transporter DTX6 confers paraquat resistance in Arabidopsis

Xia, Jianjun; Nazish, Tahmina; Javaid, Ayesha; Ali, Mohsin; Liu, Qianqian; Wang, Liang; Zhang, Zheng-Yi; Zhang, Zisheng; Huang, Yongping; Wu, Jie; Yang, Zhisen; Sun, Linfeng; Yuxing, Chen; Xiang, Cheng‐Bin

doi:10.1016/j.molp.2021.09.004

Cited by 17 publications

(19 citation statements)

References 43 publications

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“…They also speculated that a small proportion of PAR1 may be localized in the chloroplast and may transport PQ into the chloroplast from the cytosol ( Li et al., 2013 ). A nonsense mutation in the coding region of PAR1 resulting in enhanced PQ tolerance in the Arabidopsis pqr2 mutant compared with the wild type ( Dong et al., 2016 ) and more than 20 par1 alleles isolated by Xia et al. (2021) reinforce the idea that PAR1 is a major locus of PQ resistance in Arabidopsis .…”

Section: Arabidopsis Mutants With Enhanced Pq Resistance Con...supporting

confidence: 53%

“…Enhanced activities of the relevant exporters would increase PQ resistance. The recently identified MATE efflux transporter DTX6 demonstrated this molecular mechanism ( Lv et al., 2021 ; Xia et al., 2021 ). The overexpression of DTX6D in Arabidopsis confers strong resistance to a near-commercial PQ dose ( Xia et al., 2021 ).…”

Section: Potential Strategies For Developing Pq-resistant Cropsmentioning

confidence: 95%

“…The reduced PQ tolerance of the knockout mutant dtx6 and the enhanced tolerance of transgenic plants overexpressing DTX6 support the notion that PQT15/DTX6 is a PQ efflux transporter. The DTX6 protein is mainly localized in the plasma membrane, consistent with the role of DTX6 as an efflux transporter ( Xia et al., 2021 ). DTX6 belongs to the MATE (multidrug and toxic compound extrusion) family of transporters that use the membrane electrochemical gradient to drive transport activities ( Kuroda and Tsuchiya, 2009 ).…”

Section: Arabidopsis Mutants With Enhanced Pq Resistance Con...mentioning

confidence: 99%

“…The recently identified MATE efflux transporter DTX6 demonstrated this molecular mechanism ( Lv et al., 2021 ; Xia et al., 2021 ). The overexpression of DTX6D in Arabidopsis confers strong resistance to a near-commercial PQ dose ( Xia et al., 2021 ). Third, PQ inside plant cells could be catabolized to nontoxic metabolites, a much-welcomed mechanism of herbicide resistance that also alleviates the food safety concern of herbicide residues.…”

Section: Potential Strategies For Developing Pq-resistant Cropsmentioning

confidence: 99%

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Understanding paraquat resistance mechanisms in Arabidopsis thaliana to facilitate the development of paraquat-resistant crops

Nazish

Huang

Zhang

et al. 2022

Plant Communications

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Section: Arabidopsis Mutants With Enhanced Pq Resistance Con...supporting

confidence: 53%

Section: Potential Strategies For Developing Pq-resistant Cropsmentioning

confidence: 95%

Section: Arabidopsis Mutants With Enhanced Pq Resistance Con...mentioning

confidence: 99%

Section: Potential Strategies For Developing Pq-resistant Cropsmentioning

confidence: 99%

See 2 more Smart Citations

Understanding paraquat resistance mechanisms in Arabidopsis thaliana to facilitate the development of paraquat-resistant crops

Nazish

Huang

Zhang

et al. 2022

Plant Communications

Self Cite

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“…AtDTX1 transports alkaloids, antibiotics, and toxic compounds ( Li et al, 2002 ). AtDTX6 transports paraquat and relates to paraquat tolerance ( Xia et al, 2021 ). NtJAT1 transports alkaloids, such as nicotine ( Morita et al, 2009 ).…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Analysis of Multidrug and Toxic Compound Extruction Transporters in Grape

et al. 2022

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Grape (Vitis vinifera L.) is an important fruit crop in the world. It is used as a table grape and is also used for raisin and wine production. Grape berries accumulate secondary metabolites, such as anthocyanins, tannins, and resveratrol, which are known as functional compounds for human health. Multidrug and toxic compound extrusion transporter (MATEs) transport secondary metabolites. MATEs also transport other solutes, including organic acids, and toxic xenobiotics, depending on cation gradient and play various roles in plants. MATE comprises 300–500 amino acid residues and possesses a MATE domain and 8–12 transmembrane domains. In the present study, 59 MATE genes were identified in the grape genome, and phylogenetic analysis revealed the presence of four groups of grape MATEs (Group 1–4). Their information, such as gene structures, protein motifs, predicted subcellular localizations, and gene IDs of four genome annotations, that is, CRIBI v1, CRIBI v2, Genoscope, and Vcost v3, were annotated. The transport substrates and physiological functions of grape MATEs were estimated based on their homology with the analyzed MATEs in other plant species. Group 1 may transport toxic compounds and alkaloids, Group 2 may transport polyphenolic compounds, Group 3 may transport organic acids, and Group 4 may transport plant hormones related to signal transduction. In addition to the known anthocyanin transporters, VvMATE37 and VvMATE39, a novel anthocyanin transporter, VvMATE38 in Group 2, was suggested as a key transporter for anthocyanin accumulation in grape berry skin. VvMATE46, VvMATE47, and VvMATE49 in Group 3 may contribute to Al3+ detoxification and Fe2+/Fe3+ translocation via organic acid transport. This study provides helpful and fundamental information for grape MATE studies and resolves the confusion of gene IDs in different genome annotations.

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Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat

Lyu

Cao²,

Huang³

et al. 2022

aBIOTECH

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Weeds are a major biotic constraint that can cause dramatic crop production losses. Herbicide technology has been widely used by farmers as the most cost-effective weed control measure, and development of new strategy to improve herbicide tolerance in plants is urgently needed. The CRISPR/Cas9-based genome editing tool has been used in diverse applications related to agricultural technology for crop improvement. Here we identified three polyamine uptake transporter (PUT) genes in rice that are homologous to the Arabidopsis AtRMV1. We successfully demonstrate that CRISPR/Cas9-targeted mutagenesis of OsPUT1/2/3 greatly improves paraquat resistance in rice without obvious yield penalty. Therefore, manipulation of these loci could be valuable for producing transgene-free rice with improved herbicide resistance in future.

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A gain-of-function mutation of the MATE family transporter DTX6 confers paraquat resistance in Arabidopsis

Cited by 17 publications

References 43 publications

Understanding paraquat resistance mechanisms in Arabidopsis thaliana to facilitate the development of paraquat-resistant crops

Understanding paraquat resistance mechanisms in Arabidopsis thaliana to facilitate the development of paraquat-resistant crops

Genome-Wide Analysis of Multidrug and Toxic Compound Extruction Transporters in Grape

Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat

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