A 'G' chromosome banding study of three cupped oyster species: Crassostrea gigas, Crassostrea angulata and Crassostrea virginica (Mollusca: Bivalvia)

Leitão, Alexandra; Thiriot-Quiévreux, Catherine; Boudry, Pierre; Malheiro, Isabel

doi:10.1186/1297-9686-31-5-519

Cited by 21 publications

(22 citation statements)

References 24 publications

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“…Furthermore, genetic differences have now been observed on several levels. Karyotype analyses highlighted the close genetic similarity of these 2 taxa in comparison with other cupped oyster species (Leitão et al 1999a), although differences between C. angulata and C. gigas were observed, notably for Chromosome 7 (Leitão et al 1999b). Two studies on the mitochondrial Cytochrome Oxidase Subunit I (COI) gene (Boudry et al 1998, O'Foighil et al 1998) demonstrated a clear genetic difference (nucleotide divergence of 5.26%) between the 2 taxa.…”

Section: Introductionmentioning

confidence: 94%

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C. angulata highlighted by sequence variation in flanking regions of a microsatellite locus

Huvet¹,

Fabioux²,

McCombie³

et al. 2004

Mar. Ecol. Prog. Ser.

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The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several mutational events, which suggested null alleles and homoplasy. The presence of 1 insertion/deletion event in its 5' flanking sequence enabled us to design a new bi-allelic ('C' and 'NC') nuclear PCR-restriction fragment length polymorphism (-RFLP) marker (CG44R). This, together with a mitochondrial DNA marker, was used to analyze populations of C. angulata and C. gigas. The CG44R allele frequencies were very different between C. angulata (f[C] = 0.91) and C. gigas (f[NC] = 0.92) populations. This analysis also provided evidence for hybridization between C. angulata and C. gigas in a wild Portuguese population where the 2 taxa are in contact due to recent transportation of C. gigas stocks for aquacultural production. Our results represent the first indication of hybridization between these 2 taxa in the natural environment, and contribute to knowledge of the evolutionary history of the Crassostrea genus.

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Section: Introductionmentioning

confidence: 94%

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C. angulata highlighted by sequence variation in flanking regions of a microsatellite locus

Huvet¹,

Fabioux²,

McCombie³

et al. 2004

Mar. Ecol. Prog. Ser.

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“…In C. gigas, several banding techniques were applied to chromosomes, in order to identify them individually and to establish a standardized karyotype. G-banding pattern (Rodríguez-Romero et al 1979;Leitão et al 1999Leitão et al , 2001) and chromosome banding with restriction enzymes (Leitão et al 2004;Bouilly et al 2005;Cross et al 2005) have also been carried out in Crassostrea oysters. Recently, a molecular cytogenetics approach based on in situ hybridization of bacteriophage P1 clones was also used for chromosome-specific probes in C. virginica (Wang et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)4, (GT)7 and (TA)10 chromosome patterns

Bouilly

Chaves

Leitão

et al. 2008

J Genet

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Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT) 4 , (GT) 7 and (TA) 10 onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa.

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“…Probably due to the small chromosome size and the lack of cell lines required for making elongated chromosomes, chromosome banding in oysters is difficult to obtain and reproduce. C-and Gbanding patterns have been produced in three Crassostrea species (Rodriguez-Romero et al, 1979;Leitão et al, 1999a), but offered little help in the reliable identification of oyster chromosomes. Ag-NOR staining is also variable and often shows intraspecific variations in number and location, which poses problems for interspecific comparisons (Thiriot-Quiévreux and Insua, 1992;Ladrón De Guevara et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Differences in the rDNA-Bearing Chromosome Divide the Asian-Pacific and Atlantic Species ofCrassostrea(Bivalvia, Mollusca)

Wang

Guo

2004

The Biological Bulletin

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Karyotype and chromosomal location of the major ribosomal RNA genes (rDNA) were studied using fluorescence in situ hybridization (FISH) in five species of CRASSOSTREA: three Asian-Pacific species (C. gigas, C. plicatula, and C. ariakensis) and two Atlantic species (C. virginica and C. rhizophorae). FISH probes were made by PCR amplification of the intergenic transcribed spacer between the 18S and 5.8S rRNA genes, and labeled with digoxigenin-11-dUTP. All five species had a haploid number of 10 chromosomes. The Atlantic species had 1-2 submetacentric chromosomes, while the three Pacific species had none. FISH with metaphase chromosomes detected a single telomeric locus for rDNA in all five species without any variation. In all three Pacific species, rDNA was located on the long arm of Chromosome 10 (10q)--the smallest chromosome. In the two Atlantic species, rDNA was located on the short arm of Chromosome 2 (2p)--the second longest chromosome. A review of other studies reveals the same distribution of NOR sites (putative rDNA loci) in three other species: on 10q in C. sikamea and C. angulata from the Pacific Ocean and on 2p in C. gasar from the western Atlantic. All data support the conclusion that differences in size and shape of the rDNA-bearing chromosome represent a major divide between Asian-Pacific and Atlantic species of CRASSOSTREA: This finding suggests that chromosomal divergence can occur under seemingly conserved karyotypes and may play a role in reproductive isolation and speciation.

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A 'G' chromosome banding study of three cupped oyster species: Crassostrea gigas, Crassostrea angulata and Crassostrea virginica (Mollusca: Bivalvia)

Cited by 21 publications

References 24 publications

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C. angulata highlighted by sequence variation in flanking regions of a microsatellite locus

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C. angulata highlighted by sequence variation in flanking regions of a microsatellite locus

Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)4, (GT)7 and (TA)10 chromosome patterns

Differences in the rDNA-Bearing Chromosome Divide the Asian-Pacific and Atlantic Species ofCrassostrea(Bivalvia, Mollusca)

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