A fusogenic peptide expressed on the surface of Salmonella enterica elicits CTL responses to a dengue virus epitope

Lurı́a-Pérez, Rosendo; Cedillo‐Barrón, Leticia; Santos-Argumedo, Leopoldo; Ortiz-Navarrete, Vianney; Ocaña-Mondragón, Alicia; González-Bonilla, Cesar R.

doi:10.1016/j.vaccine.2007.03.047

Cited by 25 publications

(28 citation statements)

References 46 publications

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“…Although we did not evaluate the individual effect of each construction step on this immune response, we used a systematic approach based on an extensive body of literature and our previous studies to engineer an efficient ovalbumin reporter system. Among the many autotransporter proteins that are capable of presenting heterologous surface antigens or secreted proteins, MisL has already demonstrated its adaptability as an antigen delivery system in the context of experimental Salmonella vaccines [7-10,38]. Some of these studies showed that MisL was tolerant to short epitope multimers.…”

Section: Discussionmentioning

confidence: 99%

Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella

et al. 2014

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BackgroundAutotransporter proteins represent a treasure trove for molecular engineers who modify Gram-negative bacteria for the export or secretion of foreign proteins across two membrane barriers. A particularly promising direction is the development of autotransporters as antigen display or secretion systems. Immunologists have been using ovalbumin as a reporter antigen for years and have developed sophisticated tools to detect specific T cells that respond to ovalbumin. Although ovalbumin-expressing bacteria are being used to trace T cell responses to colonizing or invading pathogens, current constructs for ovalbumin presentation have not been optimized.ResultsThe activation of T helper cells in response to ovalbumin was improved by displaying the OVA-CD4 reporter epitope as a multimer on the surface of Salmonella and fused to the autotransporter MisL. Expression was optimized by including tandem in vivo promoters and two post-segregational killing systems for plasmid stabilization.ConclusionsThe use of an autotransporter protein to present relevant epitope repeats on the surface of bacteria, combined with additional techniques favoring stable and efficient in vivo transcription, optimizes antigen presentation to T cells. The technique of multimeric epitope surface display should also benefit the development of new Salmonella or other enterobacterial vaccines.

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Section: Discussionmentioning

confidence: 99%

Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella

et al. 2014

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“…Moreover, the insertion of proteolytic cleavage sites between antigenic sequences in the gene of interest to generate fused proteins or peptides might be important in CTL processing. Similarly, recombinant proteins containing fusogenic sequences provide a promising system to induce cytotoxic T cells by live-vector vaccines by destabilizing the phagosome membrane so that epitopes can reach the cytosol 82 .…”

Section: Enhancing Protein Productionmentioning

confidence: 99%

DNA vaccines: ready for prime time?

2008

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Since the discovery, over a decade and a half ago, that genetically engineered DNA can be delivered in vaccine form and elicit an immune response, there has been much progress in understanding the basic biology of this platform. A large amount of data has been generated in preclinical model systems, and more sustained cellular responses and more consistent antibody responses are being observed in the clinic. Four DNA vaccine products have recently been approved, all in the area of veterinary medicine. These results suggest a productive future for this technology as more optimized constructs, better trial designs and improved platforms are being brought into the clinic.

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“…Moreover, recombinant strains with surface displayed viral proteins retained their invasiveness, suggesting that the recombinant S. typhimurium can be used as live vaccine vector for eliciting complete immunogenicity. The data may yield better understanding of the mechanism by which ice nucleation protein displays foreign proteins in the Salmonella strain.Bacterial strains with surface expression of viral proteins can be used for the development of a diagnostic system for viral diseases or a live oral vaccine against those diseases [11][12][13] , and for the studies of the interaction between virus and host by simulation [2,15] . Many bacterial cell surface localized molecules can act as display motifs to target heterologous proteins on their surface.…”

mentioning

confidence: 99%

“…Bacterial strains with surface expression of viral proteins can be used for the development of a diagnostic system for viral diseases or a live oral vaccine against those diseases [11][12][13] , and for the studies of the interaction between virus and host by simulation [2,15] . Many bacterial cell surface localized molecules can act as display motifs to target heterologous proteins on their surface.…”

mentioning

confidence: 99%

“…Virus antigen presentation can be enhanced by a surface-displayed mechanism: a fusion protein encompassing a dengue virus epitope can reach the cytosol of CTLs through destabilizing the membrane of a phagosome containing surface-displayed Salmonella, then the virus epitope can be processed through the MHC class I-dependent pathway to stimulate CTLs similar to a living virus [15] . However, there remains uncertainty in using INP-based cell surface display with Salmonella strains, in particular there are concerns regarding the integrity of foreign protein and the display efficiency of different display motifs.…”

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confidence: 99%

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Surface display of domain III of Japanese encephalitis virus E protein on Salmonella typhimurium by using an ice nucleation protein

et al. 2011

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A bacterial cell surface display technique based on an ice nucleation protein has been employed for the development of live vaccine against viral infection. Due to its ubiquitous ability to invade host cells, Salmonella typhimurium might be a good candidate for displaying viral antigens. We demonstrated the surface display of domain III of Japanese encephalitis virus E protein and the enhanced green fluorescent protein on S. typhimurium BRD509 using the ice nucleation protein. The effects of the motif in the ice nucleation protein on the effective display of integral protein were also investigated. The results showed that display motifs in the protein can target integral foreign protein on the surface of S. typhimurium BRD509. Moreover, recombinant strains with surface displayed viral proteins retained their invasiveness, suggesting that the recombinant S. typhimurium can be used as live vaccine vector for eliciting complete immunogenicity. The data may yield better understanding of the mechanism by which ice nucleation protein displays foreign proteins in the Salmonella strain.

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A fusogenic peptide expressed on the surface of Salmonella enterica elicits CTL responses to a dengue virus epitope

Cited by 25 publications

References 46 publications

Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella

Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella

DNA vaccines: ready for prime time?

Surface display of domain III of Japanese encephalitis virus E protein on Salmonella typhimurium by using an ice nucleation protein

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