A Fusarium graminearum xylanase expressed during wheat infection is a necrotizing factor but is not essential for virulence

Sella, Luca; Gazzetti, Katia; Faoro, Franco; Odorizzi, Silvana; D’Ovidio, R.; Schäfer, Wilhelm; Favaron, Francesco

doi:10.1016/j.plaphy.2012.12.008

Cited by 64 publications

(69 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The p-value for positive selection using M8 and M8e models was 8.2e -2 , 3.8e -4 and 3.0e -1 for SS1G_07749, SS1G_10165 and SS1G_10617 respectively (Table 2). Interestingly, SS1G_07749 encodes a putative xylanase that may function as a virulence factor such as xylanases from other fungi [9, 52]. To get insights on the constraints shaping the evolution of SS1G_07749 xylanase, we predicted the 3D structure of SS1G_07749 mature protein and mapped the local Ka/Ks ratios on this structure (Figure 3b).…”

Section: Resultsmentioning

confidence: 99%

Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum

et al. 2014

View full text Add to dashboard Cite

BackgroundThe white mold fungus Sclerotinia sclerotiorum is a devastating necrotrophic plant pathogen with a remarkably broad host range. The interaction of necrotrophs with their hosts is more complex than initially thought, and still poorly understood.ResultsWe combined bioinformatics approaches to determine the repertoire of S. sclerotiorum effector candidates and conducted detailed sequence and expression analyses on selected candidates. We identified 486 S. sclerotiorum secreted protein genes expressed in planta, many of which have no predicted enzymatic activity and may be involved in the interaction between the fungus and its hosts. We focused on those showing (i) protein domains and motifs found in known fungal effectors, (ii) signatures of positive selection, (iii) recent gene duplication, or (iv) being S. sclerotiorum-specific. We identified 78 effector candidates based on these properties. We analyzed the expression pattern of 16 representative effector candidate genes on four host plants and revealed diverse expression patterns.ConclusionsThese results reveal diverse predicted functions and expression patterns in the repertoire of S. sclerotiorum effector candidates. They will facilitate the functional analysis of fungal pathogenicity determinants and should prove useful in the search for plant quantitative disease resistance components active against the white mold.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-336) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum

et al. 2014

View full text Add to dashboard Cite

show abstract

“…This is consistent with the results obtained with the F . graminearum xylanase FGSG_03624, which contributes significantly to total xylanase activity, but does not contribute to pathogenicity (Sella et al ., ). VmFAEC1D1 double‐deletion mutants exhibited reduced pathogenicity, but not reduced overall FAE activity, suggesting that the loss of VmFAEC1 and VmFAED1 did not affect the degradation of ethyl ferulate in MSEFA medium(MS‐ethyl ferulate‐agar), but substrates of the apple tree bark cell wall.…”

Section: Discussionmentioning

confidence: 97%

The feruloyl esterase genes are required for full pathogenicity of the apple tree canker pathogen Valsa mali

Gao

Chen

et al. 2017

Molecular Plant Pathology

View full text Add to dashboard Cite

Apple Valsa canker, caused by the fungus Valsa mali, is one of the most destructive diseases of apple trees in East Asia. Feruloyl esterases (ferulic acid esterases, FAEs), which belong to a subclass of carboxylic esterases, can cleave ester bonds that crosslink hydroxycinnamic acids and arabinoxylans or certain pectins in plant cell walls. However, a pathogenic role of FAE has not been demonstrated in plant-pathogenic fungi. In this study, the FAE gene family, including one type A, one type B, three type C and two type D FAE genes, was identified in V. mali. Five of the seven FAE genes had highly elevated transcript levels in V. mali-apple tree bark interactions compared with mycelia grown in axenic culture. Signal peptides of the VmFAEs were confirmed using yeast signal sequence trap assays. To examine whether FAEs are required for the pathogenicity of V. mali, seven single- and six double-gene deletion mutants were generated. Compared with the wild-type, three of the seven FAE single-deletion mutants showed significantly reduced pathogenicity and three of the six FAE double-deletion mutants exhibited greater reductions in pathogenicity, suggesting the joint action of FAEs in the V. mali-apple tree interaction. Most of the FAE mutants that exhibited a significant reduction in pathogenicity had significantly lower FAE activity than the wild-type fungus. These results indicate that secreted FAEs are required for the full pathogenicity of the phytopathogenic fungus V. mali.

show abstract

“…LIP1 ) and endoxylanases (e.g. XYLA ), have been demonstrated to breach plant defences and elicit immune reactions in plants in the hemibiotrophic fungus F. graminearum (Feng et al ., ; Sella et al ., ). Recently, Sperschneider et al .…”

Section: Identification Of Fungal Effectorsmentioning

confidence: 97%

Large‐scale molecular genetic analysis in plant‐pathogenic fungi: a decade of genome‐wide functional analysis

Motaung

Saitoh

Tsilo

2017

Molecular Plant Pathology

View full text Add to dashboard Cite

Plant-pathogenic fungi cause diseases to all major crop plants world-wide and threaten global food security. Underpinning fungal diseases are virulence genes facilitating plant host colonization that often marks pathogenesis and crop failures, as well as an increase in staple food prices. Fungal molecular genetics is therefore the cornerstone to the sustainable prevention of disease outbreaks. Pathogenicity studies using mutant collections provide immense function-based information regarding virulence genes of economically relevant fungi. These collections are rich in potential targets for existing and new biological control agents. They contribute to host resistance breeding against fungal pathogens and are instrumental in searching for novel resistance genes through the identification of fungal effectors. Therefore, functional analyses of mutant collections propel gene discovery and characterization, and may be incorporated into disease management strategies. In the light of these attributes, mutant collections enhance the development of practical solutions to confront modern agricultural constraints. Here, a critical review of mutant collections constructed by various laboratories during the past decade is provided. We used Magnaporthe oryzae and Fusarium graminearum studies to show how mutant screens contribute to bridge existing knowledge gaps in pathogenicity and fungal-host interactions.

show abstract

A Fusarium graminearum xylanase expressed during wheat infection is a necrotizing factor but is not essential for virulence

Cited by 64 publications

References 57 publications

Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum

Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum

The feruloyl esterase genes are required for full pathogenicity of the apple tree canker pathogen Valsa mali

Large‐scale molecular genetic analysis in plant‐pathogenic fungi: a decade of genome‐wide functional analysis

Contact Info

Product

Resources

About